In Vitro Antimicrobial Activity of Some Medicinal Plants against Human Pathogenic Bacteria

Manandhar, Sarita; Luitel, Shisir; Dahal, Raj Kumar

doi:10.1155/2019/1895340

Cited by 269 publications

(169 citation statements)

References 15 publications

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“…This observed antibacterial activity of organic solvent extracts can be due to the accessibility of solvent to more bioactive component of the plant leaf which could have acted singly or in combination. Medicinal plants have been known to possess antimicrobial properties against multidrug resistant bacteria and thus offers a prospective solution to the problem of reduced efficacy of antibiotics [34] and confirms the traditional use of C. odorata in the treatment of wounds [35]. Although water extract exhibited no inhibitory activity, methanol and ethanol extracts of C. odorata showed inhibitory activity against bacteria tested.…”

Section: Resultsmentioning

confidence: 70%

Evaluation of Phytochemical Constituents and Antibacterial Activity of Chromolaena Odorata L. Leaf Extract against Selected Multidrug Resistant Bacteria Isolated from Wounds

Alabi

Olusola-Makinde

Oladunmoye

2020

SAJRM

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Aim: To study the antibacterial effect of Chromolaena odorata extracts on multidrug resistant Staphylococcus aureus, Pseudomonas aeruginosa and Klebsiella pneumoniae from wounds. Place of Study: University of Medical Sciences Teaching Hospital, Akure, Ondo State, Nigeria, between January and June 2019. Methodology: A total of 87 wound swabs were collected from patients of University of Medical Sciences Teaching Hospital, Akure. Air-dried and powdered C. odorata leaves were extracted using hot water, ethanol and methanol as extraction solvents and concentrated using a rotary evaporator. The concentrated C. odorata extracts were purified using chromatographic techniques. Qualitative and quantitative phytochemical screening of C. odorata extracts were done by standard methods. Antibiotics susceptibility pattern of bacterial isolates to a panel of ten (10) conventional antibiotics was determined by disc diffusion method. Results: C. odorata methanolic extract had the highest extract yield (26.2%). From the multidrug resistance analysis, 66.7% of bacterial isolates tested had multidrug resistance index (MDRI) of 100%. Pseudomonas aeruginosa was susceptible to 100 mg/ml of C. odorata ethanolic extract but resistant (0.00±0.00) to 100 mg/ml of hot aqueous C. odorata extract. Conclusion: This study reveals the inhibitory activities of C. odorata extracts on multidrug resistant bacteria isolated from wounds and an indication of their potential in the treatment of wound infection.

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Section: Resultsmentioning

confidence: 70%

Evaluation of Phytochemical Constituents and Antibacterial Activity of Chromolaena Odorata L. Leaf Extract against Selected Multidrug Resistant Bacteria Isolated from Wounds

Alabi

Olusola-Makinde

Oladunmoye

2020

SAJRM

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“…Phytochemical screening was performed to test for alkaloids, saponin, tannins, flavonoids, steroids, sugars and cardiac glycosides [9][10] .…”

Section: Phytochemical Screening Of the Extractmentioning

confidence: 99%

Untitled

2020

IJPSR

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Plants have served as a source of new pharmaceutical products and inexpensive starting materials for the synthesis of some known drugs. Components with medicinal properties from plants play an important role in conventional Western medicine. In the ethnopharmacological approach, local knowledge about the potential uses of the plants is very useful as compared to the random approach where indigenous knowledge is not taken into consideration. In the present study, the polar (hydro-alcoholic, aqueous, methanolic and ethanolic) extracts and non-polar extracts (hexane, chloroform and petroleum ether) of whole plant, Hyptis suaveolens was screened for antimicrobial activity against drug-resistant strains of Staphylococcus aureus and other pathogenic strains (viz. Pseudomonas aeruginosa, Bacillus cereus, Aspergillus niger and Candida albicans). All the polar extracts of the plant showed significant antimicrobial activity in comparison to non-polar extracts. The studies revealed that the polar extracts are having much significant antimicrobial activity against drug-resistant strains and other pathogenic strains while non-polar extracts are having moderate activity against pathogenic strains while no activity was found against drug-resistant strains. The polar extracts showed potent antibacterial activity against multidrug-resistant Staphylococcus aureus, Pseudomonas aeruginosa, Aspergillus niger and Candida albicans in the ranges from 5.0 mg/ml to 15.0 mg/ml. The highest antimicrobial fractions of Hyptis suaveolens were chromatographed on 2 × 30 cm silica gel 60 open column using a stepwise gradient of methanol and increasing amount of ethyl acetate (20% at each step); ethyl acetate with increasing amount of methanol (10% at each step); and finally at 40% methanol. Collected fractions were evaporated under vacuum and examined by TLC. The antimicrobial fractions were examined using silica gel coated TLC plates to confirm the pure compound by changing the ratios of the solvent system components.

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“…However, the emergence of multidrug-resistant (MDR) pathogenic bacteria and their continuous increase has become a health concern. This is mainly because of the limited antimicrobial agents available to treat the infections caused by these pathogens (Luitel and Dahal 2019). However, many medicinal plants have been identified as alternative natural sources of antimicrobial agents that could be used in the treatment of these infections (Luitel and Dahal 2019).…”

Section: Introductionmentioning

confidence: 99%

“…This is mainly because of the limited antimicrobial agents available to treat the infections caused by these pathogens (Luitel and Dahal 2019). However, many medicinal plants have been identified as alternative natural sources of antimicrobial agents that could be used in the treatment of these infections (Luitel and Dahal 2019). The World Health Organization (WHO) stated that medicinal plants would be the best source to obtain a variety of drugs against the MDR bacteria (Yadav et al 2009).…”

Section: Introductionmentioning

confidence: 99%

“…The World Health Organization (WHO) stated that medicinal plants would be the best source to obtain a variety of drugs against the MDR bacteria (Yadav et al 2009). A number of medicinal plants have been used because of their antimicrobial traits due to the presence of various phytochemicals in them (Altemimi et al 2017;Luitel and Dahal 2019).…”

Section: Introductionmentioning

confidence: 99%

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In vitro antimicrobial activity of lignan from the stem bark of Strombosia grandifolia Hook.f. ex Benth

et al. 2019

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Background: The continuous spread of multidrug-resistant and new strains of disease-causing microbe have become a great concern to the world health community. There is therefore the need for the development of new and effective drugs for the treatment of these diseases. Traditional medicinal plants used in our community could be a good source of drugs to fight these problems. This is why this study is focused on the antimicrobial properties of Strombosia grandifolia used as traditional medicines in Nigeria. Results: The first reported investigation into the phytochemical constituents of S. grandifolia led to the isolation of (-)-8-hydroxypinoresinol from the CH 2 Cl 2 extract of the stem bark of the plant. The compound was identified as (-)-8-hydroxypinoresinol using 1D and 2D NMR spectroscopic methods and by comparison with literature data. The compound was active against tested microorganisms which included Staphylococcus aureus, Streptococcus pneumoniae, Escherichia coli, Salmonella typhi and Candida albicans using ciprofloxacin and terbinafine as standards. The zones of inhibition (ZOI) ranged between 11 and 17 mm for the compound against the microorganisms. The compound had a minimum inhibitory concentration (MIC) ranging from 0.75 to 3.0 mg/ml, and minimum bactericidal concentration (MBC) of 1.5, and a minimum fungicidal concentration (MFC) of 3.0 mg/ml. The lignan also showed antifungal activity against C. albicans. This experiment confirmed the efficacy of the lignan as a natural antimicrobial and suggested the possibility of employing it in drugs for the treatment of infectious diseases caused by the test organisms. This is the first report of the isolation of this compound from Strombosia grandifolia. Conclusion: In this study, antimicrobial activities of (-)-8-hydroxypinoresinol isolated from the stem bark of Strombosia grandifolia used in Nigeria for the treatment of various ailments were assessed. The result showed potential antibacterial effects of the phytochemical against bacterial strains tested. The compound also exhibited antifungal activity against C. albicans. This justifies the ethnomedicinal uses of the plant in Nigeria.

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In Vitro Antimicrobial Activity of Some Medicinal Plants against Human Pathogenic Bacteria

Cited by 269 publications

References 15 publications

Evaluation of Phytochemical Constituents and Antibacterial Activity of Chromolaena Odorata L. Leaf Extract against Selected Multidrug Resistant Bacteria Isolated from Wounds

Evaluation of Phytochemical Constituents and Antibacterial Activity of Chromolaena Odorata L. Leaf Extract against Selected Multidrug Resistant Bacteria Isolated from Wounds

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In vitro antimicrobial activity of lignan from the stem bark of Strombosia grandifolia Hook.f. ex Benth

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