In vitro antifungal susceptibility of clinical isolates of Fusarium from Colombia

Gaviria-Rivera, Adelaida; Giraldo-López, Alejandra; Cano, Luz Elena

doi:10.15446/rsap.v19n6.54654

Cited by 4 publications

(10 citation statements)

References 24 publications

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“…Moreover, Gaviria‐Rivera et al 42 tested the susceptibility of clinical isolates of Fusarium species that most of them isolated from nail (84%) to itraconazole, amphotericin B and voriconazole. In agreement with our results, they found the highest MICs values of itraconazole.…”

Section: Discussionmentioning

confidence: 99%

Prevalence, genetic diversity and antifungal susceptibility profiles of F. fujikuroi, F. solani and Fusarium incarnatum‐equiseti species complexes from onychomycosis in north of Iran

Haghani

Shams-Ghahfarokhi

Dalimi

et al. 2022

Mycoses

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Onychomycosis, a nail fungal infection, is normally caused by dermatophytes.However, yeasts and non-dermatophyte moulds (NDM) are among pathogens that cause nail disease. Regarding, this study aimed to describe the molecular epidemiology of Fusarium onychomycosis in the North of Iran. Two hundred and fifty seven nail samples collected from the patients clinically suspected of onychomycosis were subjected to direct microscopy, calcofluor white staining and culture. Fusarium isolates were identified at a species level through determination of multi-locus sequences for internal transcribed spacer and translation elongation factor 1 alpha. Based on the findings, Fusarium species were isolated from onychomycosis patients (n = 27).According to a previous partial genes analysis, the species in the recent study belonged to the members of F. fujikuroi species complex (n = 14), Fusarium incarnatumequiseti species complex (n = 1) and F. solani species complex (n = 12). In this study, F. proliferatum was the dominant Fusarium species collected from the samples. The correct identification of Fusarium species is essential regarding the increased prevalence of Fusarium onychomycosis and the inherent resistance of these agents to a wide spectrum of antifungals. The obtained results indicated variation in the epidemiology of Fusarium species isolated from onychomycosis. Moreover, the minimum inhibitory concentration (MIC) of luliconazole and lanoconazole was in the range of 0.001-1 μg/ml, with the geometric mean of MICs obtained at 0.0103 and 0.0343 μg/ ml against Fusarium species, respectively. These findings can increase researchers' knowledge regarding diversity of species, distribution of onychomycosis and the choice of a proper treatment.

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Section: Discussionmentioning

confidence: 99%

Prevalence, genetic diversity and antifungal susceptibility profiles of F. fujikuroi, F. solani and Fusarium incarnatum‐equiseti species complexes from onychomycosis in north of Iran

Haghani

Shams-Ghahfarokhi

Dalimi

et al. 2022

Mycoses

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“…The lack of this type of characterization has been a serious problem for physicians as proper identification is necessary for an adequate treatment (24). These fungi have variable susceptibility to antifungals and some of them are resistant to all the available treatments (9,12,25). Furthermore, it is necessary to compare the susceptibility of each isolate with a given genotype given the high variability at the intraspecific level (8).…”

Section: Evaluación De Marcadores De Genotipificación En La Caracteri...mentioning

confidence: 99%

“…Once growth was observed, the macroscopic colonies identified as Fusarium sp. were stored in sterile vials (25).…”

Section: Isolatesmentioning

confidence: 99%

“…We evaluated a group of 44 isolates for their susceptibility to the in vitro activity of amphotericin b, itraconazole, and voriconazole using the disk diffusion test according to the CLSI M38-A methods as previously reported (25). Isolates were grown on PDA agar for a week at room temperature.…”

Section: Antifungal Susceptibility Testsmentioning

confidence: 99%

“…To test the clustering classifications obtained with the M13 and (GACA) 4 markers and their associations with epidemiological variables we used a hypergeometric test. We evaluated the isolation tissue, the sequencing-based identification at the species level, the antimycotic sensibility in µg/ml, and the antimycotic phenotype (resistant, sensible-dose-dependent, and sensible) as reported in a previous characterization of these isolates (25). We controlled for false discovery rate adjusting by the Benjamini-Hochberg procedure (39).…”

Section: Clustering Enrichment Analysismentioning

confidence: 99%

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Assessment of genotyping markers in the molecular characterization of a population of clinical isolates of Fusarium in Colombia

et al. 2022

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Introduction: Fusarium is a very heterogeneous group of fungi, difficult to classify, with a wide range of living styles, acting as saprophytes, parasites of plants, or pathogens for humans and animals. Prevalence of clinical fusariosis and lack of effective treatments have increased the interest in the precise diagnosis, which implies a molecular characterization of Fusarium populations.Objective: We compared different genotyping markers in their assessment of the genetic variability and molecular identification of clinical isolates of Fusarium.Materials and methods: We evaluated the performance of the fingerprinting produced by two random primers: M13, which amplifies a minisatellite sequence, and (GACA)4, which corresponds to a simple repetitive DNA sequence. Using the Hunter Gaston Discriminatory Index (HGDI), an analysis of molecular variance (AMOVA), and a Mantel test, the resolution of these markers was compared to the reference sequencing-based and PCR genotyping methods.Results: The highest HGDI value was associated with the M13 marker followed by (GACA)4. AMOVA and the Mantel tests supported a strong correlation between the M13 classification and the reference method given by the partial sequencing of the transcription elongation factor 1-alpha (TEF1-α) and rDNA 28S.Conclusion: The strong correlation between the M13 classification and the sequencingbased reference together with its higher resolution demonstrates its adequacy for the characterization of Fusarium populations.

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In vitro antifungal susceptibility profile of Iranian Fusarium isolates: Emphasising on the potent inhibitory effect of efinaconazole compared to other drugs

Nosratabadi

Faeli

Haghani

et al. 2022

Mycoses

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Background Fusarium species are opportunistic human pathogens that remarkably cause fungal infections ranging from superficial to fatal invasive disseminated infections. Fusarium species are notoriously resistant to the majority of antifungal agents. Objectives Therefore, detailed studies regarding in vitro susceptibility are required and may lead to a better prognosis of severe infections. Methods We evaluated 25 antifungal drugs in vitro against 282 clinical and environmental Fusarium isolates. Results Fusarium species demonstrated high MICs/MECs values to the most commonly used antifungal drugs in clinical practice. The geometric mean (GM) MICs for luliconazole (0.004 μg/ml) and lanoconazole (0.012 μg/ml) were the lowest, followed by efinaconazole (0.98 μg/ml) and amphotericin B (1.04 μg/ml). Conclusions Efinaconazole, a novel triazole, may be a promising candidate for the treatment of superficial Fusarium infections. Furthermore, the development of systemic formulations of these drugs as well as further in vitro and in vivo investigations could aid in the treatment of systemic fusariosis.

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In vitro antifungal susceptibility of clinical isolates of Fusarium from Colombia

Abstract: The results confirm the high level of resistance reported, regardless of the species or the strain of Fusarium involved. The high MICs level observed are worrying and suggest that new drugs are needed.

Cited by 4 publications

References 24 publications

Prevalence, genetic diversity and antifungal susceptibility profiles of F. fujikuroi, F. solani and Fusarium incarnatum‐equiseti species complexes from onychomycosis in north of Iran

Prevalence, genetic diversity and antifungal susceptibility profiles of F. fujikuroi, F. solani and Fusarium incarnatum‐equiseti species complexes from onychomycosis in north of Iran

Assessment of genotyping markers in the molecular characterization of a population of clinical isolates of Fusarium in Colombia

In vitro antifungal susceptibility profile of Iranian Fusarium isolates: Emphasising on the potent inhibitory effect of efinaconazole compared to other drugs

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