In Vitro Antifungal Activity against Oral Candida Species Using a Denture Base Coated with Silver Nanoparticles

Abstract

Although oralCandidaeasily adheres to denture base materials, many denture detergents are effective only against bacteria but not againstCandida. Silver nanoparticles (AgNPs), which are known to have potent antibacterial and antifungal activity, have been used in the prevention of oral candidiasis (OC). We evaluated the adherence ofCandida albicansandCandida glabrataon a heat-cured Acron resin piece supported by AgNPs by low-vacuum scanning electron microscopy (SEM) and measuring colony-forming units.C. albicansandC. glabrataincreasingly adhered to the resin surface of the control piece over time, but the adhesion AgNP of bothCandidaspecies to the AgNP-coated surface was significantly inhibited (P<0.001). Low-vacuum SEM revealed thatC. albicansandC. glabrataon the resin surface of control pieces appeared as oval colonies, with a major axis of 3-4 μm and a smooth cell wall, but those on the AgNP-coated resin surface were less abundant than the control and showed swollen yeast features, with a major axis of more than 5 μm and a corrugated cell wall. Our results suggest a way to prevent denture-associated OC by using denture base materials processed by AgNPs.

Antimicrobial and mechanical acrylic resin properties with silver particles obtained from Fusarium oxysporum

“…These results are in agreement with previous study results that shown potent antifungal effects of AgNPs coating of denture base material -morphology deformation and inhibition of Candida adherence [26].…”

Anti-Biofilm Effect of Nanometer Scale Silver (NmSAg) Coatings on Glass and Polystyrene Surfaces against P. Mirabilis, C. Glabrata and C. Tropicalis Strains

“…When the inhibition effect of NmSAg coating on glass slabs against C. glabrata strain's, biofilm formation was examined; 20.9% inhibition was observed ( Fig. 1) and similar to our result, AgNPs are generally indicated to inhibit the adhesion of Candida to the denture surface according to the literature and are known to have an important role in preventing Candidarelated biofilm formation in the oral cavity (26,27).…”

Surface Characterization, Antimicrobial Activity, and Biocompatibility of Autopolymerizing Acrylic Resins Coated with Reynoutria elliptica Extract

“…Applying a coating resin to the denture surface not only improves the mechanical and chemical properties of the material, but also demonstrates beneficial factors, such as biological resistance to oral pathogens and color change resistance [ 3 ]. Previous studies on the surface modification of acrylic resin-based materials have been continuously undertaken, postulating the inhibition of growth and the death of oral pathogenic microorganisms [ 46 , 47 , 48 , 49 ]. In addition, numerous studies have attempted to inhibit the attachment of microorganisms to material surfaces [ 50 ].…”