Anti-Biofilm Effect of Nanometer Scale Silver (NmSAg) Coatings on Glass and Polystyrene Surfaces against P. Mirabilis, C. Glabrata and C. Tropicalis Strains

Şahal, Gülcan; Nasseri, Behzad; Bilkay, Işıl Seyis; Pi̇şki̇n, Erhan

doi:10.5301/jabfm.5000248

Cited by 7 publications

(3 citation statements)

References 28 publications

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“…These results confirm the advantages of using TiO 2 and Ag coatings in association over nanostructured 316L stainless steel in order to inhibit the growth of all types of biofilms investigated in this study (S6 to S8). Silver coatings have been successfully used as a control mechanism against diverse Candida species on glass and plastic surfaces, with 22% to 34% biofilm reduction, depending on the species [ 66 ]. This behavior can be explained on the basis of the band gap reduction of the TiO 2 due to the incorporation of AgNPs.…”

Section: Resultsmentioning

confidence: 99%

Functional Antimicrobial Surface Coatings Deposited onto Nanostructured 316L Food-Grade Stainless Steel

et al. 2021

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In our study, we demonstrated the performance of antimicrobial coatings on properly functionalized and nanostructured 316L food-grade stainless steel pipelines. For the fabrication of these functional coatings, we employed facile and low-cost electrochemical techniques and surface modification processes. The development of a nanoporous structure on the 316L stainless steel surface was performed by following an electropolishing process in an electrolytic bath, at a constant anodic voltage of 40 V for 10 min, while the temperature was maintained between 0 and 10 °C. Subsequently, we incorporated on this nanostructure additional coatings with antimicrobial and bactericide properties, such as Ag nanoparticles, Ag films, or TiO2 thin layers. These functional coatings were grown on the nanostructured substrate by following electroless process, electrochemical deposition, and atomic layer deposition (ALD) techniques. Then, we analyzed the antimicrobial efficiency of these functionalized materials against different biofilms types (Candida parapsilosis, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Staphylococcus epidermidis). The results of the present study demonstrate that the nanostructuring and surface functionalization processes constitute a promising route to fabricate novel functional materials exhibiting highly efficient antimicrobial features. In fact, we have shown that our use of an appropriated association of TiO2 layer and Ag nanoparticle coatings over the nanostructured 316L stainless steel exhibited an excellent antimicrobial behavior for all biofilms examined.

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Section: Resultsmentioning

confidence: 99%

Functional Antimicrobial Surface Coatings Deposited onto Nanostructured 316L Food-Grade Stainless Steel

et al. 2021

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“…The antibacterial activity of MℓAgNPs was determined by an agar well diffusion assay against our previously isolated and identified [ 24 ] four different clinical isolates, (Gram negative i.e., Escherichia coli , Klebsiella pneumoniae , Pseudomonas aeruginosa , and Gram positive i.e., Staphylococcus aureus ) obtained from Tertiary Care Hospital, Multan., and identified qualitatively using CHROMagar medium as reported by Sahal et al ., [ 25 ]. Four different concentrations of silver nanoparticles were used.…”

Section: Methodsmentioning

confidence: 99%

Mentha longifolia assisted nanostructures: An approach to obliterate microbial biofilms

Kazmi,

Almutairi,

Andleeb

et al. 2024

PLoS ONE

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Microbes maneuver strategies to become incessant and biofilms perfectly play a role in scaling up virulence to cause long-lasting infections. The present study was designed to assess the use of an eco-friendly formulation of functionalized silver nanoparticles generated from Mentha longifolia leaf extract (MℓE) for the treatment of biofilm-producing microbes. Nanoparticles synthesized using MℓE as a reducing agent were optimized at different strengths of AgNO3 (1 mM, 2 mM, 3 mM, and 4 mM). Synthesis of M. longifolia silver nanoparticles (MℓAgNPs) was observed spectrophotometrically (450 nm) showing that MℓAgNPs (4 mM) had the highest absorbance. Various techniques e.g., Fourier transforms Infrared spectroscopy (FTIR), Dynamic light scattering (DLS), zeta potential (ZP), X-ray Diffraction (XRD), scanning electron microscope (SEM), and transmission electron microscope (TEM) were used to characterize MℓAgNPs. In the present study, the Kirby—Bauer method revealed 4mM was the most detrimental conc. of MℓAgNPs with MIC and MBC values of 0.62 μg/mL and 1.25 μg/mL, 0.03 μg/mL and 0.078 μg/mL, and 0.07 μg/mL and 0.15 μg/mL against previously isolated and identified clinical strains of Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, and Staphylococcus aureus, respectively. Moreover, the MℓAgNP antibiofilm activity was examined via tissue culture plate (TCP) assay that revealed biofilm inhibition of up to 87.09%, 85.6%, 83.11%, and 75.09% against E. coli, P. aeruginosa, K. pneumonia, and S. aureus, respectively. Herbal synthesized silver nanoparticles (MℓAgNPs) tend to have excellent antibacterial and antibiofilm properties and are promising for other biomedical applications involving the extrication of irksome biofilms. For our best knowledge, it is the first study on the use of the green-synthesized silver nanoparticle MℓAgNP as an antibiofilm agent, suggesting that this material has antibiotic, therapeutic, and industrial applications.

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“…Silver nanoparticles offer one of the better alternatives, it has been demonstrated that they function as broad-spectrum antimicrobial agent and do not lead to the development of resistant bacterial strain [2]. Previous results have been reported were authors prepare and evaluate a PMMA/AgNps composites using different approaches [11][12][13]. The aim of this study was to incorporate silver nanoparticles on the surface of a PMMA (Opti-cryl®) substrate by using a spray method and to evaluate the antimicrobial effectiveness, mechanical properties, and solubility of silver of the final material.…”

Section: Introductionmentioning

confidence: 99%

Simple and rapid method for silver nanoparticles incorporation in polymethyl methacrylate (PMMA) substrates

Campos

DeAlba-Montero

Ruíz

et al. 2017

Superficies y Vacio

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Due to its antimicrobial properties, silver has been used in many areas of medicine and today silver nanoparticles have been incorporated into different biomaterials. The objective of this study was to implement a simple method for the incorporation of silver nanoparticles in polymethyl methacrylate substrates, to determine their mechanical properties, antimicrobial functionality and the solubility of silver incorporated. Using this approach an antimicrobial material can be obtained; the surface of a commercial polymethyl methacrylate (Opti-cryl®) was impregnated with silver nanoparticles (AgNps) using a spray deposition method (0.03% by weight). The antimicrobial activity of the material was evaluated using the Japanese industrial standard (JIS Z 2801) against Escherichia coli and Staphylococcus aureus. Inductively coupled plasma optical spectrometry (ICP-OES) was used to measure the solubility of the incorporated silver nanoparticles. Mechanical tests of flexural strength were performed to observe changes in mechanical properties. The antimicrobial results show that PMMA / AgNps has significant antimicrobial activity, showing better results for Escherichia coli. ICP-OES analysis suggests low solubility in silver nanoparticles. Mechanical tests showed a 1.6% increase in flexural strength for PMMA added with silver nanoparticles. The method presented for incorporating silver nanoparticles into PMMA and producing an acrylic antimicrobial substrate is easy and has the advantage of improving its mechanical properties.

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Anti-Biofilm Effect of Nanometer Scale Silver (NmSAg) Coatings on Glass and Polystyrene Surfaces against P. Mirabilis, C. Glabrata and C. Tropicalis Strains

Cited by 7 publications

References 28 publications

Functional Antimicrobial Surface Coatings Deposited onto Nanostructured 316L Food-Grade Stainless Steel

Functional Antimicrobial Surface Coatings Deposited onto Nanostructured 316L Food-Grade Stainless Steel

Mentha longifolia assisted nanostructures: An approach to obliterate microbial biofilms

Simple and rapid method for silver nanoparticles incorporation in polymethyl methacrylate (PMMA) substrates

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