In vitro anticancer activity of fucoidan from Turbinaria conoides against A549 cell lines

Marudhupandi, Thangapandi; Kumar, T. T. Ajith; Lakshmanasenthil, S.; Suja, G; Vinothkumar, Thirumalairaj

doi:10.1016/j.ijbiomac.2014.10.005

Cited by 73 publications

(30 citation statements)

References 27 publications

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“…3434 cm −1 , 3428 cm −1 , 3409 cm −1 , and 3433 cm −1 here) can be attributed to the stretching vibrations of the hydroxyl group (-OH) common to all polysaccharides [13]. Stretch bands at 2926 cm −1 and 2925 cm −1 indicated C-H stretching of the pyranoid ring and C-6 group of fucose and galactose [28]. The bands at 2138 and 2144 cm −1 corresponds to C-H stretching [8,29].…”

Section: Ft-ir Analysis Of Fucoidansmentioning

confidence: 68%

“…and secondary O-sulfate groups characteristic to marine-sulfated polysaccharides and stands for asymmetric stretching vibrations of sulfate esters (S=O) [8,28,33]. Absorption bands at 1054, 1051, and 1062 cm −1 correspond to the stretching vibrations of C-O-C and C-O-H groups [33,34] while the band 1098 cm −1 in F 3 graph corresponds to C-O and C-C stretching vibrations of pyranose ring.…”

Section: Gopidas and Subramanimentioning

confidence: 99%

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In Vitro Antioxidant and Cytotoxic Properties of Fucoidan From Three Indian Brown Seaweeds

Gopidas

Subramani

2019

Asian J Pharm Clin Res

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Objective: In the present study, fucoidan extracted from three brown algae, Sargassum wightii, Turbinaria ornata, and Padina tetrastromatica, was purified, characterized, and evaluated for antioxidant and cytotoxic properties. Methods: Algal powders were sequentially extracted with five solvents based on polarity and residue was subjected to acidic extraction. The filtrates were precipitated for alginates, and resultant supernatant was precipitated for fucoidan. The precipitate was centrifuged; pellet dialyzed and lyophilized to yield crude fucoidan, which was purified by diethylaminoethyl cellulose chromatography and characterized by biochemical tests and Fourier-transform infrared (FT-IR) spectrometry. Solvent extracts and fucoidans were subjected to 2,2-diphenyl-1-picrylhydrazyl assay. Fucoidans were subjected to trypan blue cytotoxicity assay. Results: Antioxidant activity was highest in methanol extracts and Padina crude fucoidan, while lowest in hexane extracts and purified Sargassum fucoidan. Sargassum yielded the highest amount of fucoidan (7.14%). Total carbohydrates increased as Sargassum> Padina > Turbinaria, sulfates as Padina > Turbinaria > Sargassum, and protein content was 0.16±0.001%. Cytotoxicity increased in a dose-dependent manner; the highest and lowest for Padina at 200 mg mL-1 (40%) and 10 mg mL-1 (4%), respectively. Antioxidant and cytotoxic properties exhibited a positive correlation with sulfate content. FT-IR spectral values were characteristic to fucoidan. Conclusion: Fucoidans from the three algae effectively scavenged free radicals and showed good cytotoxic activity. There was a positive correlation between sulfate content and bioactivity of fucoidans, supporting its structure-function relationship. Thus, extracts and fucoidans from these algae are found to be potential candidates for pharmacological applications.

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Section: Ft-ir Analysis Of Fucoidansmentioning

confidence: 68%

Section: Gopidas and Subramanimentioning

confidence: 99%

In Vitro Antioxidant and Cytotoxic Properties of Fucoidan From Three Indian Brown Seaweeds

Gopidas

Subramani

2019

Asian J Pharm Clin Res

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“…Utilization of polysaccharides from marine origin has been used as therapeutic agents and for manufacturing antibiotics has attracted the attention of scientists in the recent years due to the less toxic and effective biological activities [7]. In general polysaccharides were classified based on their source (plant, animal and microbes) of isolation.…”

Section: Marine Polysaccharides An Effective Immunostimulant For Contmentioning

confidence: 99%

Polysaccharides in Aquatic Disease Management

Thangap¹,

Marudhup²,

Inbakandan³

2015

Fish Aquac J

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“…13 Girish et al 14 have found the potential activity of Pavonia odorata Willd extract against human breast and lung cancers. Breast cancer is also effectively inhibited by 21 reported the cytotoxicity of fucoidan from a tropical brown seaweed, Turbinaria sp. collected from Tamilnadu India against the human lung cancer A549 cell line.…”

Section: Introductionmentioning

confidence: 99%

Cytotoxicity of Fucoidan from Three Tropical Brown Algae Against Breast and Colon Cancer Cell Lines

Isnansetyo¹,

Lutfia²,

Nursid³

et al. 2016

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Introduction:Fucoidan is a sulfated polysaccharide that has a wide range of bioactivities including anti-cancer. This polysaccharide commonly is extracted from marine brown seaweed. There is lack of information on the fucoidan extracted from tropical brown algae and its anti-cancer activity. Objectives: The objectives of this study were to purify fucoidan from Sargassum sp., Turbinaria sp. and Padina sp., and to evaluate their cytotoxicity against breast cancer (MCF-7) and colon cancer cells (WiDr). Materials and Methods: Fucoidan extraction was conducted by using acid extraction method. Purified fucoidans were obtained by DEAE cellulose column chromatography and confirmed by HPLC and FT-IR spectrometry. The cytotoxicity was evaluated by using the MTT (3-[4,5-dimethylthiazol-2-yl] -2,5-diphenyltetrazolium bromide) assay. Results: Fucoidan from Sargassum sp. and Turbinaria sp. showed low cytotoxicity with IC 50 ranging between 461-663 µg/mL. Higher cytotoxicity against MCF-7 and WiDr was showed by fucoidan from Padina sp. with IC 50 of 144 and 118 µg/mL, respectively. While its IC 50 against Vero cells was 501 µg/mL.Standard fucoidan from Fucus vesiculosus exhibited IC 50 of 60, 63 and 211 µg/mL against MCF-7, WiDr and Vero Cells, respectively. Although the IC 50 was higher than that of standard fucoidan, Padina sp. fucoidan showed cytotoxicity comparable with standard fucoidan at concentrations below 100 µg/mL. Conclusion: These results indicated that Padina sp.fucoidan showed potential selective cytotoxicity, and promising for the development of an anti-cancer compound.

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In vitro anticancer activity of fucoidan from Turbinaria conoides against A549 cell lines

Cited by 73 publications

References 27 publications

In Vitro Antioxidant and Cytotoxic Properties of Fucoidan From Three Indian Brown Seaweeds

In Vitro Antioxidant and Cytotoxic Properties of Fucoidan From Three Indian Brown Seaweeds

Polysaccharides in Aquatic Disease Management

Cytotoxicity of Fucoidan from Three Tropical Brown Algae Against Breast and Colon Cancer Cell Lines

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