In-vitro anticancer activity against Caco-2 cell line of colloidal nano silver synthesized using aqueous extract of Eucalyptus Camaldulensis leaves

Abstract

In the current study, we investigated the anticancer potential against human colon cells (Caco-2) of colloidal nanosilver (CN–Ag) produced in Syria using bioactive compounds in the aqueous extract of Eucalyptus camaldulensis leaves (AEECL). The formation of AgNPs was confirmed by UV-visible spectroscopy analysis with surface plasmon peak at 449 nm and their average size was found to be 12, 10, 23 nm by SEM, DLS and NTA respectively. This small size has confirmed the effective role of AEECL as capping agent. Further morphological characterization was done by EDS showed the presence of metallic silver. Zeta potential value (-23 mV) indicated the repulsion among the particles and stability of the formulation nanosilver. The anticancer effect of synthesized CN–Ag against Caco-2 has been tested. The cytotoxicity assay showed a dose-dependent and a time-dependent effect of CN–Ag. The high cytotoxicity of CN–Ag at low concentration (5μ/mL) open new prospects for the development of novel therapeutic approaches against human colon cancer Caco-2.

Biosynthesis of Silver Nanoparticles by Marine Actinobacterium Nocardiopsis dassonvillei and Exploring Their Therapeutic Potentials

“…The results showed that Bio-AgNPs at a concentration of 23.45 μg/ml reduced CaCo2 cell viability to 50%, which was determined to be the IC 50 . After 24 h of incubation, the IC 50 of AgNPs on the CaCo2 cell line was determined to be 18 μg/ml ( Zein et al, 2020 ). AgNPs have been shown to have a variety of cytotoxic effects in a variety of cell types, indicating that they harmed cell survival by disrupting mitochondrial structure and metabolism ( Manivasagan et al, 2013 ; Składanowski et al, 2016 ).…”

Green Synthesis, Characterization of Gold Nanomaterials using Gundelia tournefortii Leaf Extract, and Determination of Their Nanomedicinal (Antibacterial, Antifungal, and Cytotoxic) Potential

“…Exposure time, charge, degree of accumulation, concentration, the chemistry of surface composition, shape, and size be an able significant effect on the toxicity of AuNPs [48]. It was reported that the concentrations above 3.75 μg/mL and 5 μg/mL of NPs showed toxic effects on Caco-2 cells [49]. In other studies, suppressor concentrations in Skov-3 cells were reported as 9.4 μg/mL [50] and 29.36 μg/mL [51].…”

Synthesis and characterization of the novel pyrimidine’s derivatives, as a promising tool for antimicrobial agent and in-vitro cytotoxicity

“…The toxicity due to treatment with synthesized compounds may be attributed to apoptotic effects, DNA fragmentation, reduced potential for mitochondrial membrane, reactive oxygen species (ROS) generation, nuclear fragmentation, and inactivation of some enzymes such as lactate dehydrogenase [69][70][71]. In the current study, the hydrophobic chain that exists in the pyrimidine ring has a critical role to explain their cytotoxicity [72].…”