In vitro antibacterial properties of Cinnamomum zeylanicum essential oil against clinical extensively drug-resistant bacteria

Saki, Morteza; Seyed‐Mohammadi, Sakineh; Montazeri, Effat Abbasi; Siahpoosh, Amir; Moosavian, Mojtaba; Latifi, Seyed Mahmoud

doi:10.1016/j.eujim.2020.101146

Cited by 30 publications

(13 citation statements)

References 29 publications

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“…The isolation, characterization, and identification of E. coli, K. pneumoniae , and P. aeruginosa from the abattoir and poultry samples was carried out using standard microbiology techniques including culture techniques, microscopy, biochemical testing, morphological and colonies features of the bacteria on selective culture media [ 34 ]. The isolates were bacteriologically, biochemically and microscopically confirmed to be E. coli, Klebsiella pneumoniae , and P. aeruginosa .…”

Section: Methodsmentioning

confidence: 99%

Metallo-β-lactamase and AmpC genes in Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa isolates from abattoir and poultry origin in Nigeria

Chika

Nworie²,

Saki

et al. 2021

BMC Microbiol

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Background Gram-negative bacteria (GNB) including Escherichia coli, Pseudomonas aeruginosa, and Klebsiella pneumoniae represent the most relevant reservoir of resistance genes such as metallo-β-lactamase (MBL) and AmpC genes that give them the undue advantage to resist antimicrobial onslaught. This study aimed to investigate the occurrence of MBL (blaIMP-1, blaIMP-2, blaVIM-1, blaVIM-2) and AmpC (blaFOX, blaDHA, blaCMY, blaACC) resistance genes in aforementioned GNB collected from abattoir and poultry sources in Nigeria. Results In total, 370 isolates were collected from abattoir tables (n = 130), anal region of cows (n = 120), and the cloacae of poultry birds (n = 120). The test isolates showed high rate of resistance to cephalosporins and carbapenems. The MBLs were phenotypically detected in 22 E. coli, 22 P. aeruginosa, and 18 K. pneumoniae isolates using combined disc test (CDT). However, only 11 E. coli, 24 P. aeruginosa, and 18 Klebsiella pneumoniae isolates were phenotypically confirmed to be AmpC producers using cefoxitin-cloxacillin double disk synergy test (CC-DDST). MBL encoding genes (particularly the blaIMP-1 genes and blaIMP-2 genes) were detected by polymerase chain reaction (PCR) in 12 (54.6%) E. coli, 15 (83.3%) K. pneumoniae, and 16 (72.7%) P. aeruginosa isolates. AmpC genes (particularly the blaCMY genes and blaFOX genes) were found in a total of 5 (29.4%) E. coli isolates, 5 (27.8%) isolates of K. pneumoniae, and 10 (41.7%) isolates of P. aeruginosa. Conclusions Our study showed the circulation of MBL and AmpC genes in GNB from abattoir and poultry origin in Nigeria. Adoption of regular control policies is necessary to reduce the spread of these species as soon as possible, especially in poultry and slaughterhouses.

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Section: Methodsmentioning

confidence: 99%

Metallo-β-lactamase and AmpC genes in Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa isolates from abattoir and poultry origin in Nigeria

Chika

Nworie²,

Saki

et al. 2021

BMC Microbiol

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“…They have become more alluring to biologists by virtue of their myriad biological activities, and, on top of this, their antimicrobial and immunomodulatory effects [ 37 , 38 ]. However, most studies investigated their antibacterial activities against standard strains, food-borne pathogens and food-spoiling bacteria [ 39 ]. In light of this, the present study aims to investigate the resistance modulatory effect of cinnamic and gallic acids combined with various antibiotics on MDR A. baumannii clinical isolates as well as study the effect of gallic acid on the transcription of biofilm-related genes ( bap , csuE , ompA ) and its verification with in silico studies.…”

Section: Introductionmentioning

confidence: 99%

In Silico Docking, Resistance Modulation and Biofilm Gene Expression in Multidrug-Resistant Acinetobacter baumannii via Cinnamic and Gallic Acids

et al. 2022

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Despite the mounting global burden of antimicrobial resistance (AMR), the generation of new classes of effective antimicrobials still lags far behind. The interplay between multidrug resistance and biofilm formation in Acinetobacter baumannii has drastically narrowed the available therapeutic choices. The use of natural compounds holds promise as an alternate option for restoring the activity of existing antibiotics and attenuating virulence traits through reduced biofilm formation. This study aimed to evaluate the modulatory effect of combining cinnamic and gallic acids at ½MIC with various antibiotics against multidrug-resistant (MDR) A. baumannii clinical isolates as well as study the effect on the expression of the biofilm-associated genes (bap, csuE, ompA) via quantitative, real-time PCR. Combining cinnamic or gallic acid with imipenem, amikacin or doxycycline resulted in significant reduction of resistance (p < 0.05). On the contrary, no effect was recorded when both acids were combined with levofloxacin, and only cinnamic acid had a synergistic effect with colistin. The transcriptomic changes of biofilm-related genes in the presence of gallic acid at ½MIC were compared with untreated control samples. The fold expression values proved that gallic acid substantially down-regulated the respective genes in all five strong biofilm formers. Molecular docking studies of gallic and cinnamic acids on target genes revealed good binding affinities and verified the proposed mechanism of action. To the best of our knowledge, this is the first report on the effect of gallic acid on the expression of bap, csuE and ompA genes in A. baumannii, which may permit its use as an adjunct anti-virulence therapeutic strategy.

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“…The increasing failure of chemotherapeutics recently mandated the quest for newer and less expensive antimicrobials effective against disease-causing microorganisms (Kotra and Mobashery, 1998;Thomson and Moland, 2000;Saki et al, 2020;Sevindik, 2020). The search for inexpensive but potent antimicrobial is essential for the low-and middle-income African countries including Nigeria, that are currently hit by the menace of multidrug resistance and infectious diseases (Okeke et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Bioactivity of Four Nigerian Wild Mushrooms against Some Typed Clinical Isolates

2022

JJBS

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This study aimed at investigating the antimicrobial activity of some wild Nigerian mushrooms against selected typed clinical isolates. We collected wild mushrooms from an integrated organic farm in Ilesa, Southwest Nigeria. Crude methanolic extracts of Lentinus squarrosulus Mont., Termitomyces robustus (Beeli) R. Heim, Trametes ochracea (Pers.) Gilb. & Ryvarden and Xylaria hypoxylon (L.) Grev. were screened singly and in different combinations for bioactivity against the selected bacterial and yeast isolates. The minimum inhibitory concentration (MIC) and chemical constituents of the extracts were studied following standard procedures. Overall, we obtained a total of 16 mushrooms belonging to 14 genera. The extracts showed varied clearance zones against at least one of the eight bacteria, and one yeast when applied singly with the antimicrobial inhibitory zone ranging from 7.2 mm to 20.0 mm in Staphylococcus aureus (T. ochracea extract) and Pseudomonas aeruginosa (L. squarrosulus extract) respectively. Furthermore, the MIC ranged from 2.09 to 16.75 mg/mL. When combined, the blends were active against some Gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa and Proteus mirabilis) but inactive against the Gram-positive bacteria and yeast. Except for X. hypoxylon, other extracts contained saponins, tannins and terpenoids. Our findings revealed that the wild mushrooms are potential antimicrobial agents against the tested isolates.

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In vitro antibacterial properties of Cinnamomum zeylanicum essential oil against clinical extensively drug-resistant bacteria

Cited by 30 publications

References 29 publications

Metallo-β-lactamase and AmpC genes in Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa isolates from abattoir and poultry origin in Nigeria

Metallo-β-lactamase and AmpC genes in Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa isolates from abattoir and poultry origin in Nigeria

In Silico Docking, Resistance Modulation and Biofilm Gene Expression in Multidrug-Resistant Acinetobacter baumannii via Cinnamic and Gallic Acids

Bioactivity of Four Nigerian Wild Mushrooms against Some Typed Clinical Isolates

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