In vitro Antibacterial Effect of 2% Chlorhexidine Against Enterococcus faecalis in Dentin Previously Irrigated with 5% Sodium Hypochlorite

Abstract

In vitro antibacterial effect of 2% chlorhexidine against Enterococcus faecalis in dentin previously irrigated with 5% sodium hypochlorite. Int. J. Odontostomat., 9(1):25-29, 2015. ABSTRACT:The aim of this study was to observe whether the antibacterial effect of 2% chlorhexidine against Enterococcus faecalis in dentine is altered by previous irrigation with 5% sodium hypochlorite. Dentin discs were prepared with different irrigation protocols: group 1, immersed in 2% CHX for 25 min; group 2 immersed in 5% NaOCl for 25 minutes; group 3, immersed in 5% NaOCl, dried and irrigated with 3 ml of 2% CHX; group 4 and 5 immersed in 5% NaOCl, rinsed with 5 and 25 ml of distilled water respectively, dried and irrigated with 2% CHX. Group 6, immersed in 0.9% sodium chloride. Discs were then placed in agar plates in which E. faecalis was grown and the inhibition zone around each disc was measured after 24 hours of incubation at 37 ºC. All experimental groups showed E. faecalis growth inhibition. The most effective irrigant was 2% CHX (P<0.05). Groups in which both NaOCl and CHX were used displayed significantly smaller inhibition halos as compared with 2% CHX. Different volumes of water for rinsing did not cause significant improvement in growth inhibition. The antimicrobial effect of 2% chlorhexidine against E. faecalis was significantly reduced when dentin was previously irrigated with 5% sodium hypochlorite despite of rinsing with different volumes of water.

Antibacterial Activity of Endodontic Gutta-Percha—A Systematic Review

“…The most significant antimicrobial effects are achieved when using gutta-percha with chlorhexidine, indicating the greatest reduction in bacterial cultures after contact with this substance. Study results indicated that chlorhexidine is effective against Enterococcus faecalis, a notion supported by numerous other studies demonstrating both the in vitro and in vivo effectiveness of this aspect [40][41][42][43][44][45][46][47]. Furthermore, chlorhexidine exhibits biocidal activity against Gram-positive bacteria, and, if the minimum inhibitory concentration (MIC) is maintained, it can also inhibit Gram-negative bacteria.…”

Soluções irrigadoras utilizadas no preparo químico-mecânico do sistema de canais radiculares: uma revisão da literatura

“…Vem se mostrando como possível substituto do NaOCl por resultar uma menor toxicidade, ter ação antimicrobiana contra espécies resistentes ao NaOCl, menor tensão superficial e por demonstrar ação residual após a interação com a dentina, fenômeno conhecido como substantividade. No entanto, a CHX não apresenta um potencial de degradação dos tecidos orgânicos, uma importante condição das soluções químicas que são usadas ao longo da preparação dos canais, sendo associada com o NaOCl por preencherem as condições de uma solução irrigante ideal na endodontia (EcheverrI & Alderete, 2015).…”