In vitro antibacterial, antioxidant, anti-inflammatory and analgesic evaluation of Rosmarinus officinalis L. flower extract fractions

Karadağ, Ayşe Esra; Demırcı, Betül; Çaşkurlu, Ayşegül; Demirci, Fatih; Okur, Mehmet Evren; Orak, Duygu; Sipahi, Hande; Başer, Kemal Hüznü Can

doi:10.1016/j.sajb.2019.07.039

Cited by 50 publications

(43 citation statements)

References 32 publications

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“…Rosmarinus spp. have been used in traditional medicines as an antispasmodic, diuretic, antiepileptic, carminative, renal colic, antirheumatic, expectorant, for diabetes, dysmenorrhea, heart diseases, and for relieving respiratory disorders [ 177 ]. In the United States and Europe, rosemary is the only spice commercially available for use as an antioxidant.…”

Section: Bioactive Compounds From Eos Of Spice Plantsmentioning

confidence: 99%

Bioactive Natural Compounds and Antioxidant Activity of Essential Oils from Spice Plants: New Findings and Potential Applications

et al. 2020

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Spice plants have a great influence on world history. For centuries, different civilizations have used them to condiment the foods of kings and nobles and applied them as embalming preservatives, perfumes, cosmetics, and medicines in different regions of the world. In general, these plants have formed the basis of traditional medicine and some of their derived substances have been utilized to treat different human diseases. Essential oils (EOs) obtained from these plants have been also used as therapeutic agents and have shown supportive uses in remedial practices. The discovery and development of bioactive compounds from these natural products, based on their traditional uses, play an important role in developing the scientific evidence of their potential pharmaceutical, cosmetic, and food applications. In the present review, using recent studies, we exhibit a general overview of the main aspects related to the importance of spice plants widely used in traditional medicine: Cinnamomum zeylanicum (true cinnamon), Mentha piperita (peppermint), Ocimum basilicum (basil), Origanum vulgare (oregano), Piper nigrum (black pepper), Rosmarinus officinalis (rosemary), and Thymus vulgaris (thyme); and we discuss new findings of the bioactive compounds obtained from their EOs, their potential applications, as well as their molecular mechanisms of action, focusing on their antioxidant activity. We also exhibit the main in vitro methods applied to determine the antioxidant activities of these natural products.

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Section: Bioactive Compounds From Eos Of Spice Plantsmentioning

confidence: 99%

Bioactive Natural Compounds and Antioxidant Activity of Essential Oils from Spice Plants: New Findings and Potential Applications

et al. 2020

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“…Cinnamomum verum essential oil is reported to have antimicrobial effects (Narayanan et al, 1980;Dubey and Mishra, 1990), fungitoxic effects (Tiwari et al, 1994). Karadag et al ( 2019) found that Rosmarinus officinalis L. (rosemary) is a common culinary spice and herbal drug, which is used for centuries all over the world.…”

Section: A=rosmarinus Officinalis B= Zingiber Officinale C= Cinnamomumentioning

confidence: 99%

Phytochemical and Biological Studies on Some Medicinal Plants

El-Kelany

2020

Al-Azhar Journal of Pharmaceutical Sciences

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Biological studies on thirteen ethanolic plant extracts (Rosmarinus officinalis ,Ocimum basilicum , Moringa oleifera, Zingiber officinale, Curcuma longa, Nigella sativa ,Cinnamomum verum ,Salvia officinalis, Lepidium sativum, Foeniculum vulgare, Anethum graveolens, Ficus benghalensis and Cinnamomum camphora) revealed that four of them (Rosmarinus officinalis , Zingiber officinale, Cinnamomum verum and Cinnamomum camphora) were the best active against two bacterial species ; E. coli , S .aureus and one fungus species C. albicans. Also, their synergistic effects against E. coli, S. aureus and C. albicans were studied .So, the phytochemical studies were completed on these four plants. This study aimed to evaluate the chemical composition of the best active plant extracts. The chemical major content of Rosmarinus officinalis was eucalyptol (7.48%) , Zingiber officinale was gingerol (12.73%) , Cinnamomum verum was (E)-cinnamaldehyde (25.55 %) and Cinnamomum camphora was eugenol (27 .35%). The minimum inhibitory concentration (MIC) values varied from 0.625 to 2.5 mg/ml, for the S. aureus (gram positive bacteria) affected by Rosmarinus officinali, Zingiber officinale, Cinnamomum verum and Cinnamomum camphora. Respectively. C. albicans was the most effective microorganism by Cinnamomum verum and the least effective microorganism by Rosmarinus officinalis and Cinnamomum camphora. ethanolic extracts, as MIC ranged from 0.15 to 1.25 mg/ml.

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“…The in vitro antimicrobial activity was evaluated by determining the minimum inhibitory concentrations (MIC) via the broth microdilution protocol according to the Clinical and Laboratory Standards Institute [38]. The commonly found microorganisms on colonized wounds were studied; S. aureus, P. aeruginosa, S. epidermidis, and E. coli strains were grown in Mueller Hinton Broth (MHB, Merck, Germany); C. albicans species were grown in RPMI broth at 37 • C in aerobic conditions for 24 h. Bacteria suspension was adjusted to 0.5 McFarland turbidity standards (corresponding to 10 8 colony-forming units (CFU)/mL) with sterile saline.…”

Section: Antimicrobial Activitymentioning

confidence: 99%

In Vivo Wound Healing and In Vitro Anti-Inflammatory Activity Evaluation of Phlomis russeliana Extract Gel Formulations

et al. 2020

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The air-dried aerial parts of Phlomis russeliana (Sims) Lag. Ex Benth. was extracted by methanol and fractionated by n-hexane, dichloromethane, and ethyl acetate, respectively. The wound healing properties of P. russeliana extract gel was evaluated using the in vivo excisional wound model using Balb-c mice. Initially, the P. russeliana methanol extract showed LOX inhibitory activity at IC50 = 23.2 µg/mL, whereas the DPPH• assay showed IC50 = 0.89 mg/mL, and the ABTS• assay showed IC50 = 0.99 mg/mL, respectively. In addition, a remarkable anti-inflammatory activity was observed in the cell culture assay. Thereafter, activity-guided fractionation was performed by LOX enzyme inhibition assays, and the structures of the two most active fractions were revealed by both GC–FID and GC/MS analyses, simultaneously. Phytol and 1-heptadecanoic acid were characterized as the active constituents. Moreover, the P. russeliana extract gel formulation was applied for in vivo tests, where the new gel formulation supported the in vitro anti-inflammatory activity findings. As a conclusion, this experimental results support the wound healing evidence based on the ethnobotanical application of Phlomis species with further potential.

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In vitro antibacterial, antioxidant, anti-inflammatory and analgesic evaluation of Rosmarinus officinalis L. flower extract fractions

Cited by 50 publications

References 32 publications

Bioactive Natural Compounds and Antioxidant Activity of Essential Oils from Spice Plants: New Findings and Potential Applications

Bioactive Natural Compounds and Antioxidant Activity of Essential Oils from Spice Plants: New Findings and Potential Applications

Phytochemical and Biological Studies on Some Medicinal Plants

In Vivo Wound Healing and In Vitro Anti-Inflammatory Activity Evaluation of Phlomis russeliana Extract Gel Formulations

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