In Vitro Antibacterial, Anti-Adhesive and Anti-Biofilm Activities of Krameria lappacea (Dombey) Burdet & B.B. Simpson Root Extract against Methicillin-Resistant Staphylococcus aureus Strains

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) represents a serious threat to public health, due to its large variety of pathogenetic mechanisms. Accordingly, the present study aimed to investigate the anti-MRSA activities of Krameria lappacea, a medicinal plant native to South America. Through Ultra-High-Performance Liquid Chromatography coupled with High-Resolution Mass spectrometry, we analyzed the chemical composition of Krameria lappacea root extract (KLRE). The antibacterial activity of KLRE was determined by the broth microdilution method, also including the minimum biofilm inhibitory concentration and minimum biofilm eradication concentration. Besides, we evaluated the effect on adhesion and invasion of human lung carcinoma A549 cell line by MRSA strains. The obtained results revealed an interesting antimicrobial action of this extract, which efficiently inhibit the growth, biofilm formation, adhesion and invasion of MRSA strains. Furthermore, the chemical analysis revealed the presence in the extract of several flavonoid compounds and type-A and type-B proanthocyanidins, which are known for their anti-adhesive effects. Taken together, our findings showed an interesting antimicrobial activity of KLRE, giving an important contribution to the current knowledge on the biological activities of this plant.

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“…Staphylococci with MDR are known as methicillinresistant S. aureus (MRSA) and generally, the bacteria were resistant to β-lactam antibiotics. The MRSA was formed of substitution in gen that Penicillin Binding Protein (PBP2) encoding to PBP2a, so that active side receptors for β-lactam antibiotic cannot be recognized (Genovese et al 2021). Antibiotic resistance occurred to change in the active side of amino acids into the other amino acid which occurs at several points, for example at position 106 serine change to amino acid glycine, serine 70 change to alanine and serine 42 change to asparagine (Munita and Arias 2016).…”

Bioguided Isolation of Antibiofilm and Antibacterial Pregnane Glycosides from Caralluma quadrangula: Disarming Multidrug-Resistant Pathogens

“…Methicillin-resistant Staphylococcus aureus (MRSA) and multidrug-resistant Acinetobacter baumannii (MDRAB) are opportunistic pathogens. They are well fitted to hospital environments, and the perseverance of their chronic infections is mainly due to their capability to form biofilm and resist the host immune system [1,2]. The emergence of microbial resistance to numerous conventional antibiotics has become a serious global concern.…”

“…MRSA and MDRAB are the most commonly encountered pathogens in hospitals [6,7]. Drug resistance and biofilm formation appear to play a critical condition in the pathogenicity of MRSA and MDRAB [1,2,8]. The majority of A. baumannii were found to be multidrug-resistant strains.…”

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