In vitro antibacterial activities of pomegranate extract against standard microorganisms of bovine mastitis

Abstract

Bovine mastitis is characterized by inflammation of the mammary gland, usually due to bacterial infection, compromising quantity and quality of milk production. This study aimed to determine the antibacterial activity, in vitro, of the hydroalcoholic extract of the pomegranate peel at 10% against standard strains of bovine mastitis. The colonies were adjusted to the concentration of 10 7 mL -1 using UV-visible spectrophotometry, and the extracts were evaluated in quintuplicate in concentrations of 1000, 500, 250, 75, 50 and 25 μg mL -1 . The sensitivity of the strains was determined using the minimum inhibitory concentration and disk diffusion test. Additionally, antioxidant activity and total phenolic content was evaluated. The extract, at concentrations of 500 and 1000 μg mL . Perhaps the presence of other substances in the extract may have been responsible for the antioxidant activity detected. That way, the antioxidant and antibacterial activities of EHPG 10% may represent an important therapeutic potential, particularly for animal health in organic and agroecological production systems.