In Vitro Antibacterial Activities of Root-Canal Sealers By Using Two Different Methods

Çobankara, Funda Kont; Altinöz, Halil Cenk; Erganiş, Osman; Kav, K.; Bellí, Sema

doi:10.1097/00004770-200401000-00013

Cited by 100 publications

(118 citation statements)

References 19 publications

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“…Studies report that the antimicrobial action of AH Plus is attributed to formaldehyde release over a short period of time during the setting time. 7,26,30 However, just after 14 days, AH Plus showed antimicrobial effectiveness against E. faecalis in the present study, disagreeing with Slutzky-Goldberg et al, 31 who did not find any antimicrobial action after 14 days. The different methodologies used can explain the variation in results.…”

Section: Discussioncontrasting

confidence: 93%

Antimicrobial action of calcium hydroxide-based endodontic sealers after setting, against E. faecalis biofilm

et al. 2016

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Antimicrobial action of calcium hydroxide-based endodontic sealers after setting, against E. faecalis biofilm Abstract: Enterococcus faecalis are gram positive bacteria that can mostly resist endodontic therapy, inducing persistent infection in the root canal system. Endodontic sealers with antimicrobial activity may help eliminate residual microorganisms that survive endodontic treatment. The present study aimed at comparing the antimicrobial activity of Acroseal, Sealapex and AH Plus endodontic sealers in an in vitro biofilm model. Bovine dentin specimens (144) were prepared, and twelve blocks for each sealer and each experimental time point (2, 7 and 14 days) were placed and left in contact with plates containing inoculum of E. faecalis (ATCC 51299), to induce biofilm formation. After 14 days, the samples were transferred to another plate with test sealers and kept at 37°C and 5% CO 2 for 2, 7 and 14 days. The specimens without sealers were used as a control for each period. The samples were agitated in a sonicator after each experiment. The suspensions were agitated in a vortex mixer, serially diluted in saline, and triple plated onto m-Enterococcus agar. Colonyforming units were counted, and the data were statistically analyzed using ANOVA, Shapiro-Wilk and Kruskal-Wallis one-way tests (p < 0.05) to determine antimicrobial potential. Sealapex showed significant differences at all the experimental time points, in comparison with all the other groups. AH Plus and Acroseal showed antimicrobial activity only on the 14 th experimental day. Neither of the sealers tested were able to completely eliminate the biofilm. Sealapex showed the highest antimicrobial activity in all the experimental periods. The antimicrobial activity of all the sealers analyzed increased over time.

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Section: Discussioncontrasting

confidence: 93%

Antimicrobial action of calcium hydroxide-based endodontic sealers after setting, against E. faecalis biofilm

et al. 2016

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“…In view of the high prevalence of facultative anaerobes and anaerobes in unsuccessful endodontic therapy, the antibacterial activity of root canal sealers on these microorganisms may help to eliminate residual microorganisms that are unaffected by both chemomechanical preparation and intracanal medication and may help control infection. 37 The PU-based self-curing sealers …”

Section: Dctmentioning

confidence: 99%

Physicochemical and biological properties of a novel injectable polyurethane system for root canal filling

Wang¹,

Zuo²,

Zhao³

et al. 2015

IJN

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A root canal sealer with antibacterial activity can be efficacious in preventing reinfection that results from residual microorganisms and/or the leakage of microorganisms. In the present study, a series of injectable, self-curing polyurethane (PU)-based antibacterial sealers with different concentrations of silver phosphate (Ag 3 PO 4 ) were fabricated. Subsequently, their physicochemical properties, antibacterial abilities, and preliminary cytocompatibilities were evaluated. The results indicated that the fabricated PU-based sealers can achieve a high conversion rate in a short amount of time. More than 95% of the isocyanate group of PU sealers with 3 wt% (PU3) and 5 wt% (PU5) concentrations of Ag 3 PO 4 were included in the curing reaction after 7 hours. With the exception of those for film thickness for PU5, the results of setting time, film thickness, and solubility were able to meet the requirements of the International Organization for Standardization. The antibacterial tests showed that PU3 and PU5 exhibit stronger antimicrobial effects than that achieved with 1 wt% Ag 3 PO 4 (PU1) and AH Plus (positive control) against Streptococcus mutans . The cytocompatibility evaluation revealed that the PU1 and PU3 sealers possess good cytocompatibility and low cytotoxicity. These results demonstrate that the PU3 sealer offers good physicochemical and antimicrobial properties along with cytocompatibility, which may hold great application potential in the field of root canal fillings.

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“…The factors that affect the results of agar diffusion tests are selection of the agar medium and microorganisms, standardization of inoculation density and incubation and reading point of the zones of inhibition (Sipert et al 2005). In the present study, we used freshly mixed cements of MTA and biodentine for agar diffusion test because antimicrobial activity of different materials may be related to the degree of setting (Cobankara et al 2004). …”

Section: Discussionmentioning

confidence: 99%

Comparative evaluation of antimicrobial activity of biodentine and MTA against e. faecalis an in vitro study

Jose

Shoba

Tomy

et al. 2016

IJDR

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Background: Surgical endodontic therapy is the prescribed treatment of choice whenever the non-surgical options fail which includes the use of a root end filling material. E. faecalis is the most prevalent organism associated with the treatment failed cases Aim: The purpose of the present study was to evaluate and compare the antimicrobial efficacy of Biodentine and MTA as root end filling material and root repair material against E. faecalis. Settings and Design: Comparative invitro study was conducted at Tropical Institute of Ecological Sciences, Kottayam under Mahatma Gandhi University, Kottayam, Kerala. Materials and Methods:The antimicrobial activity was determined using agar diffusion test. Freshly mixed MTA and Biodentine was added to respective wells and incubated. The zone of inhibition of growth was recorded. Statistical analysis: The data analysis was performed using Students 't' test Results: Statistically significant difference was found between Biodentine and MTA in against E. faecalis growth. Conclusions: Biodentine was found to have superior antibacterial activity against E. faecalis than MTA. Hence it can be a better alternative to MTA as root end filling and root repair material.

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In Vitro Antibacterial Activities of Root-Canal Sealers By Using Two Different Methods

Cited by 100 publications

References 19 publications

Antimicrobial action of calcium hydroxide-based endodontic sealers after setting, against E. faecalis biofilm

Antimicrobial action of calcium hydroxide-based endodontic sealers after setting, against E. faecalis biofilm

Physicochemical and biological properties of a novel injectable polyurethane system for root canal filling

Comparative evaluation of antimicrobial activity of biodentine and MTA against e. faecalis an in vitro study

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