In vitro Anti-ulcer potential of Raphanus sativus L. seeds

Abstract

Background: Raphanus sativus L. is an indigenous plant that is traditionally used to treat peptic ulcer. Objective: This study assessed anti- Helicobacter pylori, antioxidant, and anti-urease activities of R. sativus seeds. Methods: this study targeted in-vitro model. The antioxidant activity of the methanol extract and n-butanol, aqueous, n- hexane and chloroform fractions of R. sativus seeds was determined using DPPH. Moreover, the inhibitory effects of the extract and fractions on the activity of urease and antibacterial activity against Helicobacter pylori were also evaluated. Results: The crude extract as well as its derived n-butanol fraction exhibited higher (IC50: 45 and 31 µg/mL respectively) DPPH inhibition activity and inhibition of urease (IC50: 75.0 and 55.9 µg/mL respectively) while aqueous extract inhibited H. pylori strongly (MIC50: 15 µg/mL). Conclusion: Collectively, our data show that R. sativus extract and fractions possess antioxidant, anti- H. pylori and anti-urease potential. The traditional uses of R. sativus as anti-ulcer, might be due to its anti-urease activity.