In-Vitro Anti-Proliferative, Apoptotic and Antioxidative Activities of Medicinal Herb Kalonji (Nigella sativa)

Abstract

Background: Natural product with apoptotic activity could serve as a potential new source for anti-cancer medicine. Numerous phytochemicals from plants have shown to exert antineoplastic effects via programmed cell death (apoptosis). Cancer is one of the leading causes of death in prosperous countries. The subject study was intended to evaluate the anticancer properties of Kalonji extracts against cancer cell lines HeLa and HepG2 and normal cell lines BHK and VERO were used as normal controls. Materials & Methods: For the evaluation of anti-proliferative effects, cell viability and cell death in all groups of cells were evaluated via MTT, crystal violet and trypan blue assays. For the evaluation of angiogenesis, Immunocytochemistry and ELISA of VEGF were done. Immunocytochemistry and ELISA of Annexin-V and p53 were performed for the estimation of apoptosis in all groups of cells. Furthermore, LDH assay, antioxidant enzymes activity (GSH, APOX, CAT and SOD) and RT-PCR with proliferative and apoptotic markers along with internal control were also performed. Cancer cells of both cell lines HepG2 and HeLa cells showed reduced viability, angiogenesis and proliferation with increased apoptosis when treated with Kalonji extracts. Whereas anti-oxidative enzymes show enhanced levels in treated cancer cells as compared to untreated ones. Conclusion: It was observed that Kalonji extracts have the ability to induce apoptosis and improve the antioxidant status of HeLa and HepG2 cells. They can also inhibit the proliferation and angiogenesis in both these cancer cell lines.

Synergistic Effect of Barbadensis miller and Marsdenia Condurango Extracts Induces Apoptosis Promotes Oxidative Stress by Limiting Proliferation of Cervical Cancer and Liver Cancer Cells

“…Cell viability was also assessed by crystal violet staining method. This method was conducted in a 96well plate accoding to maqbool T protocol (Maqbool et al, 2019)…”

P53-mediated in vitro inhibition of PhIP-induced oxidative damage by myricetin bulk and nano forms in healthy lymphocytes

“…1 ). The anti-oxidant, GSH activity was increased while oxidative stress was decreased enhancing the repairing capacity of the cells and positively affecting their proliferation (Maqbool et al 2019 ). Increase in GSH levels after treatment with MYR B and MYR N co-supplementation supports the narrative that myricetin might be able to initiate DDR in the presence of oxidative stress caused by PhIP.…”

Anti-Proliferative and Apoptosis-Inducing Activity of Acacia Modesta and Opuntia Monocantha Extracts on HeLa Cells

“…To calculate cytotoxicity in HeLa cells, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was performed with different concentrations onto cells cultivated in 96 well plates according to the protocol described before (Maqbool et al, 2019).…”

“…Superoxide dismutase (SOD) activity was also estimated in 96 well plates following as well-established protocol (Maqbool et al, 2019). The reaction mixture was prepared for this assay in which secretome of different experimental groups of post-treatment on HeLa cells were mixed with 100 mM KH 2 PO 4 buffer at pH 7.8, 0.1 mM EDTA, 13 mM methionine, 2.25 mM nitro-blue tetrazolium chloride (NBT), 60 µM riboflavin.…”

“…Cells of experimental groups were subjected to immunostaining by the method of Maqbool et al, (2019). The cells were washed with TBS-T three times followed by incubation in 4% para formaldehyde (PFA) for 30 min at room temperature and washed again with TBS-T five times.…”