In vitro anti-proliferative and apoptotic activity of different fractions of Artemisia armeniaca

Mojarrab, Mahdi; Lagzian, Marjan-Sadat; Emami, Seyed Ahmad; Asili, Javad; Tayarani‐Najaran, Zahra

doi:10.1590/s0102-695x2013000500010

Cited by 43 publications

(23 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, the enzyme activities of their extracts and essential oils were studied by observing acetyl‐ and butyrylcholinesterase, urease, and tyrosinase inhibitory activities (Ellman, Courtney, Andres, & Featherstone, ; Hearing & Jiménez, ; Hina et al, ; Khan et al, ). In addition, cytotoxic activities of their extracts and essential oils were determined by considering the MTT method (Mojarraba, Langzian, Emamic, Asilic, & Tayarani‐Najaranb, ; Yener et al, ).…”

Section: Methodsmentioning

confidence: 99%

Phytochemical and biological investigations on twoNepetaspecies:Nepeta heliotropifoliaandN. congestasubsp.cryptantha

et al. 2019

View full text Add to dashboard Cite

In the present study, the essential oil and aroma compositions of Nepeta heliotropifolia (NH) and N. congesta subsp. cryptantha (NC) were determined by gas chromatography-mass spectrometry (GC-MS) and gas chromatography-flame ionization detector (GC/FID), and their phenolic compounds by liquid chromatography-tandem mass spectrometry (LC-MS/MS). In addition, antioxidant, cytotoxic, anticholinesterase, urease, and tyrosinase activities of essential oils obtained from NH and NC aerial parts and ethanol extracts prepared from different parts of NH and NC were investigated. The major constituent of water-distilled essential oils was found to be germacrene D (36.7% and 38.5%, respectively), and their main aroma component was eucalyptol (48.0% and 24.7%, respectively). Among the studied parts of NH and NC, their flowers extracts were found to be the richest in phenolic compounds and in which the most abundant compound was rosmarinic acid (8,909.91 and 4,317.20 μg/g, respectively). NH and NC flower extracts exhibited also strong antioxidant activity in DPPH, ABTS, and CUPRAC assays. Among the tested samples, NH essential oil indicated the best cytotoxic effect against PDF, HT-29, and MCF-7 (IC 50 52.34, 25.89, and 44.70 μg/ml, respectively), and the highest butyrylcholinesterase (77.21 ± 1.12% inhibition) and moderate acetylcholinesterase (41.36 ± 0.69% inhibition) inhibitory activities. Practical applicationsThis is the first report on the essential oil and aroma compositions, the phenolic compounds, the antioxidant with total phenolic and flavonoid contents, cytotoxic, anticholinesterase, urease, and tyrosinase activities of Nepeta heliotropifolia and N. congesta subsp. cryptantha, except for their essential oil compositions. The reported results suggested that Nepeta heliotropifolia and N. congesta subsp. cryptantha flowers being rich in rosmarinic acid and having strong antioxidant potential, and NH essential oil possessing significant cytotoxic and butyrylcholinesterase inhibitory effect could be source for nutraceutical, food, and drug industries. K E Y W O R D Saroma, biological activities, essential oil, LC-MS/MS, Nepeta, phenolic compound 2 of 12 | AKDENIZ Et Al.

show abstract

Section: Methodsmentioning

confidence: 99%

Phytochemical and biological investigations on twoNepetaspecies:Nepeta heliotropifoliaandN. congestasubsp.cryptantha

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Hexane, butanol, and ethyl acetate fractions of P. amarus were prepared according to the method suggested by Mojarrab et al. . All extracts and fractions were stored in the refrigerator at –20°C until analysis.…”

Section: Methodsmentioning

confidence: 99%

“…This procedure was repeated three times with fresh solvent and finally a dark, greenish residue was obtained. Hexane, butanol, and ethyl acetate fractions of P. amarus were prepared according to the method suggested by Mojarrab et al [40]. All extracts and fractions were stored in the refrigerator at -20 • C until analysis.…”

Section: Extraction and Fractionationsmentioning

confidence: 99%

Development of a UHPLC–MS/MS method for the quantitation of bioactive compounds in Phyllanthus species and its herbal formulations

Kumar

Singh

Bajpai

et al. 2017

J of Separation Science

View full text Add to dashboard Cite

Phyllanthus species are extensively used in traditional medicines for the treatment of hepatic diseases due to their bioactive hypophyllanthin and phyllanthin. This work describes the development and validation of an ultra high performance liquid chromatography with tandem mass spectrometry method in polarity switching multiple reaction monitoring mode for the simultaneous detection and quantitation of 23 compounds using ultra-performance liquid chromatography coupled with electrospray-hybrid triple quadrupole-linear ion trap mass spectrometer. The validated parameters showed good linearity (R ≥ 0.996), limit of detection (0.05-1.62 ng/mL), limit of quantitation (0.15-4.95 ng/mL), precisions (intra-day: RSD ≤ 2.11%), (inter-day: RSD ≤ 2.91%), stability (RSD ≤ 2.56%) and overall recovery (98.22-104.48%; RSD ≤ 2.93%). The validated method was successfully applied in ethanolic extracts of P. amarus, P. niruri, P. emblica, P. fraternus, fractions of P. amarus and their herbal formulations for quantitation. The maximum content of hypophyllanthin (29.40 mg/g) and phyllanthin (56.60 mg/g) was detected in ethyl acetate fraction of P. amarus. The total content of 23 compounds was abundant in the ethanolic extract of P. emblica fruit. Principal component analysis was used to differentiate the selected Phyllanthus species and their herbal formulations. The results indicated that the present method could be used for quality control of Phyllanthus species and its herbal formulations.

show abstract

“…Cytotoxic activity was evaluated using MTT colorimetric method described byMojarraba, Langzian, Emamic, Asilic, and Tayarani- Najaranb (2013). Breast cancer cell line (MCF-7) and colon cancer cell line (HT-29) were used for cytotoxicity while primary dermal fibroblast cell line (PDF) for toxicity.…”

mentioning

confidence: 99%

A GC–MS method validation for quantitative investigation of some chemical markers inSalvia hypargeiaFisch. & C.A. Mey. of Turkey: Enzyme inhibitory potential of ferruginol

Bakir¹,

Akdeniz²,

Ertaş

et al. 2020

J Food Biochem

View full text Add to dashboard Cite

In the present study, a GC‐MS method used for quantitative screening of 26 compounds (sclareolide, sclareol, ferruginol, cryptanol, 6,7‐dehydroroyleanone, suginal, 9,10‐dihydro‐7,8‐dimethyl‐2‐(1‐methylethyl) phenanthren‐3‐ol, sugiol, inuroyleanone, 12‐demethylmulticauline, 7α‐hydroxy‐β‐sitosterol, stigmasterol, sitosterol, salvigenin, sinensetin, α‐amyrin, lupeol, lupenone, 3‐acetyl lupeol, 1α,21α‐dihydroxy‐2,3‐(1′1′‐dimethyl‐dioxymethylene) urs‐9(11),12‐dien, uvaol, betulin, pyxinol, lup‐(20),29‐ene‐2α‐hydroxy‐3β‐acetate, betulin 3β, 28β‐diacetate, 21α‐hydroxy,2α,3β‐diacetoxy urs‐9(11),12‐dien) specific to Turkish Salvia species was developed and validated. According to the GC–MS analysis results, Salvia hypargeia Fisch. & C.A. Mey. roots were found to be rich in ferruginol (30787.97 µg/g extract) and lupenone (23276.21 µg/g extract), and leaves in lupeol (20625.92 µg/g extract). Additionally, the essential oil and aroma contents of this species were identified by GC‐MS technique. According to the LC‐MS/MS results, especially S. hypargeia leaf extract was rich in rosmarinic acid (38035.7 µg/g extract) and isoquercitrin (4136.91 µg/g extract). Furthermore, anticholinesterase, antiurease, antityrosinase and antielastase inhibitory, antioxidant, cytotoxic activities of the ethanol extracts, essential oil, and major components of the species were evaluated. Antioxidant potentials of all extracts of this species were quite high in all studied antioxidant methods. Moreover, butyrylcholinesterase and elastase inhibitory capacities of ferruginol, the major component of S. hypargeia roots, were notable. For these reasons, this species has a high potential for food and pharmaceutical industries. Practical applications This new GC–MS method was applied to S. hypargeia Fisch. & C.A. Mey. and it indicated that this species possessed high amount of ferruginol and lupeol, and that this species could be used for their natural sources. According to the results of the activity studies (antioxidant, anticholinesterase, tyrosinase, elastase, and cytotoxic), this method was used to exhibit which compound may be responsible for the activities. This developed and validated method could be easily applied to determine major/active/toxic secondary metabolites of Salvia species which are used and/or could be used in pharmaceutical, cosmetic, and food industries.

show abstract

In vitro anti-proliferative and apoptotic activity of different fractions of Artemisia armeniaca

Cited by 43 publications

References 24 publications

Phytochemical and biological investigations on twoNepetaspecies:Nepeta heliotropifoliaandN. congestasubsp.cryptantha

Phytochemical and biological investigations on twoNepetaspecies:Nepeta heliotropifoliaandN. congestasubsp.cryptantha

Development of a UHPLC–MS/MS method for the quantitation of bioactive compounds in Phyllanthus species and its herbal formulations

A GC–MS method validation for quantitative investigation of some chemical markers inSalvia hypargeiaFisch. & C.A. Mey. of Turkey: Enzyme inhibitory potential of ferruginol

Contact Info

Product

Resources

About