In vitro anti-proliferative and antioxidant studies on Devil's Club Oplopanax horridus

Tai, Joseph; Cheung, Susan; Cheah, Stefanie; Chan, Edwin; Hasman, David

doi:10.1016/j.jep.2006.05.018

Cited by 20 publications

(17 citation statements)

References 18 publications

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“…Although recent pharmacological studies have observed that O. horridus possesses antidiabetic, antiviral, antibacterial, and cancer chemopreventive potential, chemical studies of this plant are very limited, so the active compounds were not previously identified (McCutcheon et al, 1995; Tai et al, 2006). Recently, we conducted a phytochemical isolation of O. horridus root bark and obtained a series of compounds.…”

Section: Introductionmentioning

confidence: 99%

Identification of potential anticancer compounds from Oplopanax horridus

et al. 2013

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Oplopanax horridus is a plant native to North America. Previous reports have demonstrated that this herb has antiproliferative effects on cancer cells but study mostly focused on its extract or fractions. Because there has been limited phytochemical study on this herb, its bioactive compounds are largely unknown. We recently isolated and identified 13 compounds, including six polyynes, three sesquiterpenes, two triterpenoids, and two phenolic acids, of which five are novel compounds. In this study, we systemically evaluated the anticancer effects of compounds isolated from O. horridus. Their antiproliferative effects on a panel of human colorectal and breast cancer cells were determined using the MTS assay. Cell cycle distribution and apoptotic effects were analyzed by flow cytometry. The in vivo antitumor effect was examined using a xenograft tumor model. Among the 13 compounds, strong antiproliferative effects were observed from falcarindiol and a novel compound oplopantriol A. Falcarindiol showed the most potent antiproliferative effects, significantly inducing pro-apoptosis and cell cycle arrest in the S and G2/M phases. The anticancer potential of falcarindiol was further verified in vivo, significantly inhibiting HCT-116 tumor growth in an athymic nude mouse model at 15 mg/kg. We also analyzed the relationship between polyyne structures and their pharmacological activities. We observed that both the terminal hydroxyl group and double bond obviously affected their anticancer potential. Results from this study supplied valuable information for future semi-synthesis of polyyne derivatives to develop novel cancer chemopreventive agents.

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Section: Introductionmentioning

confidence: 99%

Identification of potential anticancer compounds from Oplopanax horridus

et al. 2013

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“…The active ingredients contained in the inner bark of devil's club have been found to inhibit the growth of certain bacteria and fungi that cause a variety of illnesses, e.g., tuberculosis and fungal pneumonia (Kobaisy et al 1997, McCutcheon et al 1994. More recent studies suggest that devil's club extracts may be effective in preventing the further growth of several types of human cancer cells and have benefits as a tonic and for the treatment of arthritis, rheumatism, and perhaps adult-onset diabetes (Li et al 2010, Tai et al 2006, J. Tai pers. comm.…”

Section: Ethnobotany Research and Applicationsmentioning

confidence: 99%

Recovery of Oplopanax horridus (Sm.) Miq., an Important Ethnobotanical Resource, after Clearcut Logging in Northwestern British Columbia

Burton¹,

Burton

2015

Ethnobot. Res. App.

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“…For the negative control, the cells were treated with the MeOH only. After a 48 h incubation period, cell proliferation was determined with the CellTiter 96 Ò aqueous non-radioactive cell proliferation assay (Promega, Madison, WI) (Tai, Cheung, Cheah, Chan, & Hasman, 2006). Results were recorded by the lQuant Bio-Tek microplate reader at 490 nm.…”

Section: Cell Proliferation Assaymentioning

confidence: 99%