In vitro anti-Mycobacterium tuberculosis activity of some Brazilian "Cerrado" plants

Abstract

RESUMO: "Atividade anti-Mycobacterium tuberculosis in vitro de algumas plantas do "Cerrado" Brasileiro". O objetivo deste trabalho foi realizar uma seleção de algumas plantas de uma determinada região Brasileira com atividade contra Mycobacterium tuberculosis. Extratos clorofórmicos e metanólicos de 37 espécies de plantas distribuídas em 17 famílias do "Cerrado" Brasileiro foram avaliadas contra M. tuberculosis H 37 Rv e a Concentração Inibitória Mínima (CIM) foi determinada pelo uso do Microplate Alamar Blue Assay (MABA). Extratos brutos de dezesseis plantas apresentaram valor de CIM ≤ 125 µg/mL e três de 31,2 µg/mL. Estes resultados sugerem que o "Cerrado" Brasileiro deve possuir um recurso de plantas com constituintes ativos anti-M. tuberculosis que podem ser extraídos por solventes polares e apolares. Unitermos: Plantas do Cerrado Brasileiro, Mycobacterium tuberculosis, atividade antituberculose, MABA.ABSTRACT: The aim of this work was to carry out a screening of some plants of this Brazilian region with activity against Mycobacterium tuberculosis. Chloroform and methanol extracts of 37 plant species distributed among 17 families from Brazilian "Cerrado" which were tested against M. tuberculosis H 37 Rv and the Minimum Inhibitory Concentration (MIC) was determined by the use of Microplate Alamar Blue Assay (MABA). Crude extracts from sixteen plants showed MIC value of ≤ 125 µg/mL and three 31.2 µg/mL. These results suggest that the Brazilian "Cerrado" may be a source of plants that have activity anti-M. tuberculosis constituents that can be extracted by polars and apolars solvents.

Antioxidant properties of species from the Brazilian cerrado by different assays

“…Many research groups screen plant extracts of Brazilian biomes, like Cerrado, Atlantic Forest, Amazon and Caatinga to detect bioactive substance (Cecílio et al, 2012;Tretin et al, 2011;Mesquita et al, 2009;Pavan et al, 2009;Cardoso-Lopes, 2008;Alves et al, 2000).…”

GENES DIFFERENTIALLY EXPRESSED BY Mycobacterium tuberculosis AFTER EXPOSURE TO RUTHENIUM PHOSPHINIC COMPOUND AND ISONIAZID

“…An incomplete or inadequate treatment led to the emergence of resistant Mycobacterium tuberculosis to antituberculous (anti-TB) drugs. Studies have shown anti-TB activity by several classes of nonconventional compounds generating great expectations and hopes [3,4]. The evaluation of the effects of new compounds and nonconventional anti-TB drugs have grown and become increasingly more popular in recent years, mainly by the emergence of multidrug resistant TB (MDR-TB) and extensively-drug resistant TB (XDR-TB) [5][6][7].…”

Search for synthetic and natural substances with in vitro bioactivity against Mycobacterium tuberculosis. Part I

“…Se considera que para que sea factible continuar dentro del proceso de descubrimiento, una molécula sintética debe tener una CMI ≤ 10µg/mL o su equivalente molar (Pavan et al 2012;Bueno-Sánchez y Kouznetsov, 2010;Senthilkumar et al 2009). En el caso de las sustancias naturales, por ejemplo las de origen vegetal, si bien su composición diversa, se ha sugerido una buena actividad con CMI ˂ 100 µg/mL para los extractos crudos y ≤ 1µg/mL para los compuestos puros (Pavan et al, 2009;Bueno-Sánchez et al 2011;Bueno-Sánchez, 2012).…”