In-vitro anti-inflammatory and antioxidant activities of an Ayurvedic formulation –Trayodashang guggulu

Dadoriya, Priyanka; Dey, Yadu Nandan; Sharma, Deepti; Yadav, Mahendra Singh; Wanjari, Manish; Gaidhani, Sudesh; Subhose, Varanasi

doi:10.1016/j.hermed.2020.100366

Cited by 13 publications

(11 citation statements)

References 34 publications

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“…The KG was subjected to phytochemical screening by various phytochemical tests for the presence of various primary and secondary metabolites using standard protocols [ [25] , [26] , [27] , [28] ].…”

Section: Methodsmentioning

confidence: 99%

Development and evaluation of aphrodisiac potential of a classical ayurvedic formulation, ‘Kaamdev ghrita’ in rat model

Gurav

Wanjari

et al. 2021

Journal of Ayurveda and Integrative Medicine

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Background Ghee is widely considered as the Indian name for clarified butterfat and processing of ghee with therapeutic herbs i.e. ghrita is renowned for augmenting their medicinal properties. Kaamdev ghrita (also known as ‘ Vajikaran a Rasayana’ ) is cow ghee based classical Ayurvedic formulation from the aphrodisiac category, which is used to ameliorate and potentiate sexual performance and also in the treatment of sexual dysfunctions, infertility, and premature ejaculation. Objective Present research work deals with the organoleptic, physicochemical, and biological assessment of Kaamdev ghrita for its aphrodisiac activity using in-vivo animal models. Material and methods Kaamdev ghrita was prepared using Indian cow’s ghee as per standard Ayurvedic classical texts and subjected to organoleptic (color, odor, taste, texture, touch), physicochemical (acid value, peroxide value, iodine value, saponification value, unsaponifiable matter, extractive values, refractive index, and specific gravity) analyses as per the standard pharmacopeial procedures. The aphrodisiac potential of ghrita in rat model was evaluated by monitoring sexual behavioral performance using different parameters (mount frequency and latency, intromission frequency and latency, anogenital grooming and sniffing) at the dose of 150 and 300 mg/kg body weight. Results The physicochemical evaluation of Kaamdev ghrita showed higher acid value, iodine value, refractive index, and specific gravity whereas the lower saponification and peroxide value than the plain ghee . Kaamdev ghrita revealed the presence of flavonoids, alkaloids, saponins, sterols, terpenoids, coumarins, tannins, and showed remarkable antioxidant activity by in-vitro assays. It augmented the sexual performance in a dose-dependent manner as indicated by significant improvement (P < 0.05) in mount frequency and latency, intromission frequency and latency, anogenital grooming, and sniffing as compared to plain ghee treated control group. The present investigation has corroborated the ethnopharmacological claim of Kaamdev ghrita for its aphrodisiac potential. Conclusion Kaamdev ghrita exhibited aphrodisiac activity which may be attributed to the presence of antioxidant herbs present in it. It is the first scientific report on validation of the traditional claim of Kaamdev ghrita for its aphrodisiac potential.

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Section: Methodsmentioning

confidence: 99%

Development and evaluation of aphrodisiac potential of a classical ayurvedic formulation, ‘Kaamdev ghrita’ in rat model

Gurav

Wanjari

et al. 2021

Journal of Ayurveda and Integrative Medicine

View full text Add to dashboard Cite

show abstract

“…The antioxidant and anti-inflammatory potential of CM extract was evaluated by spectrophotometry using these in vitro models. During injury and pathogenic attack, the inflammatory response is a challenging state strongly related to discomfort and involves processes such as increased protein denaturation and cellular membrane rupture ( Dadoriya et al, 2020 ). Further, tissue inflammation manifests the body's reaction to stress ( Yuan et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

“…Multiple experiments involving the inflammatory process are performed to test the effectiveness of medicines. Well known, protein denaturation is linked to inflammatory processes ( Dadoriya et al, 2020 ). Denaturation of proteins is characterized by the loss of their tertiary and secondary structure.…”

Section: Discussionmentioning

confidence: 99%

“…This assay has been done as described previously ( Dadoriya et al 2020 ). The reaction mixture was composed of 0.2 mL of fresh hen’s egg albumin, 2.8 mL phosphate buffer saline (pH 6.4) and 2 mL of various concentrations (25–400 µg/mL) of CM extract or standard drugs.…”

Section: Methodsmentioning

confidence: 99%

“…The tube samples were kepet at 70 ˚C in the bath for 30 min. After cooling and centrifuging for 5 min at 3000 rpm, the absorbance was measured at 560 nm using UV spectrophotometer ( Dadoriya et al, 2020 ). In addition, the % inhibition of hemolysis and IC 50 values were calculated.…”

Section: Methodsmentioning

confidence: 99%

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