In vitro anti-inflammatory and anti-lipid accumulation properties of taxifolin-rich extract from the Japanese larch, Larix kaempferi

Abstract

The Japanese larch, ( Larix kaempferi ) is known to contain abundant taxifolin (dihydroquercetin) in its xylem. In this study, to assess the bioactivities of taxifolin rich methanol extract of L. kaempferi (LK-ME), anti-inflammatory effect, and the anti-lipid accumulation effect of LK-ME were investigated. The results showed that nitric oxide (NO) and reactive oxygen species (ROS) were reduced after treatment with LK-ME, and that lipid accumulation in adipocyte differentiated 3T3-L1 cells was inhibited after the cells were grown in medium containing LK-ME. Taxifolin, the major compound contained in LK-ME, and its related compounds, quercetin and luteolin also exhibited similar effects with LK-ME. The LK-ME exhibits relatively strong anti-inflammatory and anti-lipid accumulation activities compared with that of similar amounts of taxifolin contained in LK-ME, suggesting that other minor compounds contained in LK-ME is involved in the effects. These results indicate the potential of taxifolin-rich L. kaempferi extract for use as a supplement to prevent excess inflammation and obesity.

Extract of Pinus densiflora needles suppresses acute inflammation by regulating inflammatory mediators in RAW264.7 macrophages and mice

“…In previous studies, it was investigated that taxifolin inhibit ROS production (Muramatsu et al. 2020 ) and quercetin glucoside has anti-oxidative effects, such as increasing SOD level and catalase activity whereas decreasing ROS and MDA levels (Dai et al. 2018 ).…”

Chemical Composition and Antioxidant, Anti-Inflammatory, and Enzyme Inhibitory Activities of an Endemic Species from Southern Algeria: Warionia saharae

“…The phenolic compounds highly present in W. saharae extract are probably responsible for the anti-inflammatory property observed in this study, and flavonoids can be highlighted [ 42 , 68 ]. Taxifolin, the main phenolic compound present in W. saharae extract, was previously found to possess significant anti-inflammatory activity against carrageenan-induced edema involving the exudative phase of inflammation and also against formaldehyde-induced arthritis [ 69 ]. These results corroborate the traditional use of this species in folk medicine.…”

Novel Therapeutic Potentials of Taxifolin for Obesity-Induced Hepatic Steatosis, Fibrogenesis, and Tumorigenesis

“…Of note, based on previous studies [ 38 ], the dose of Taxifolin used in our in vitro study is considered to be within the physiological range, although we did not determine its serum concentrations in our mouse models. Taxifolin is also known to suppress proinflammatory cytokine expression in cultured macrophages in vitro and chemically induced liver fibrosis in vivo [ 18 , 39 ]. Several studies suggest the involvement of Nrf-2 (nuclear factor erythroid 2-related factor 2), HO-1 (heme oxygenase 1), and AMPK (AMP-activated protein kinase) as the underlying mechanism of the antioxidative properties of Taxifolin [ 8 , 40 , 41 ].…”