Chemical Composition and Antioxidant, Anti-Inflammatory, and Enzyme Inhibitory Activities of an Endemic Species from Southern Algeria: Warionia saharae

Rechek, Habiba; Haouat, Ammar; Hamaidia, Kaouther; Allal, Hamza; Boudiar, Tarek; Pinto, Diana; Cardoso, Susana M.; Bensouici, Chawki; Soltani, Noureddine; Silva, Artur M. S.

doi:10.3390/molecules26175257

Cited by 10 publications

(9 citation statements)

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“…This test followed a previously reported method [ 32 ]. In a 96-well plate, a volume of 50 μL of each sample at different concentrations (3.1–200 μg/mL) was mixed with 50 μL of CuCl 2 (10 mM), 50 μL of neocuproine (7.5 mM) and 60 μL of ammonium acetate buffer (NH 4 Ac, 1 mol/L, pH 7.0).…”

Section: Methodsmentioning

confidence: 99%

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Phlorotannins of the Brown Algae Sargassum vulgare from the Mediterranean Sea Coast

et al. 2022

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Brown seaweeds are a good source of bioactive compounds, particularly of phlorotannins, which may exert a wide spectrum of pharmacological properties. In the present study, phlorotannins of S. vulgare were extracted using a 70% acetone solution and the crude extract was further purified through liquid–liquid partition, giving rise to n-hexane, ethyl acetate and aqueous residue fractions. The crude extract and the purified fractions were evaluated for potential antioxidant abilities as well as for inhibitory potential towards the digestive enzymes α-amylase and pancreatic lipase, and anti-inflammatory potential through the hindering of albumin denaturation. Overall, the ethyl acetate fraction was the richest in phlorotannins (9.4 ± 0.03 mg PGE/g) and was also the most promising regarding the tested bioactive properties. Of note, its inhibitory potential towards α-amylase was about nine times that of the commercial drug acarbose and its inhibitory activity against high temperature-induced protein denaturation was superior to that of the non-steroidal drug ketoprofen. According to UHPLC-DAD-ESI-MS/MS analysis, this fraction contained a range of phlorotannins with at least six units of phloroglucinol, including dibenzodioxine-1,3,6,8-tetraol, fuhalol, pentaphlorethol, fucopentaphlorethol and dihydroxypentafuhalol, in addition to several less common phlorotannin sulfate derivatives.

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Section: Methodsmentioning

confidence: 99%

“…Galvinoxyl radical scavenging was determined according to the method described in [ 32 ]. Fresh galvinoxyl radical solution (0.1 mM) was prepared in methanol and mixed with 40 μL of sample.…”

Section: Methodsmentioning

confidence: 99%

Phlorotannins of the Brown Algae Sargassum vulgare from the Mediterranean Sea Coast

et al. 2022

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“…Until recently, W. saharae had not sparked the curiosity of scientists, and just a few articles on this species had been published. The hydromethanolic extract of W. saharae exhibits significant glucosidase inhibitory activities, accord-ing to Rechek et al [4]. The essential oil and crude extracts of this plant were found to have antibacterial, cytotoxic, antioxidant, and anti-inflammatory properties against the cancer cell line "KB cells" [4,5].…”

Section: Introductionmentioning

confidence: 79%

“…Afessas, abessas, or tazart nîfiss are some of the native Berber names for this plant [3]. W. saharae leaves are used in traditional medicine to treat inflammatory disorders, gastrointestinal problems, and epileptic seizures [4]. Until recently, W. saharae had not sparked the curiosity of scientists, and just a few articles on this species had been published.…”

Section: Introductionmentioning

confidence: 99%

The Antispasmodic Effect of Warionia saharae Essential Oil in Experimental Models and its Mechanism of Action

Aziz¹,

Marghich²,

Addi³

et al. 2022

Front. Biosci. (Schol Ed)

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With several medicinal and aromatic species, the Asteraceae family is one of the largest angiosperm families. The genus Warionia is represented in this family by only one species, Warionia saharae. In Moroccan traditional medicine, this species is widely used to treat gastrointestinal problems. Essential oil of this plant (EoWs) was studied for possible myorelaxant and antispasmodic activities to rationalize some of the traditional uses. In this investigation, hydrodistillation was used to obtain the essential oil from the aerial part of the dry plant extract (EoWs), which was then analyzed using gas chromatography coupled to mass spectrometry (GC/MS). The major compounds identified in the EoWs are nerolidyl acetate (21.44%), β-Eudesmol (19.47%), linalool (16.48%), 1-terpinene-4-ol (10.93%), and cineole (5.34%). EoWs is relatively safe in the case of acute intake up to 2 g/kg body weight of albino mice. The effect of EoWs on intestinal relaxation was investigated using rabbit and rat jejunal smooth muscle. We have noticed that EoWs produce a myorelaxation on basal rabbit jejunum's contractions in a concentration-dependent manner with a maximal effect at 30 µg/mL. This myorelaxation was not dependent on adrenergic receptors. When the rat jejunums were pre-contracted with 25 mM KCl or 10 µM Carbachol (CCh), EoWs had an antispasmodic action with an IC50 values of 15.76 ± 0.37 and 12.04 ± 0.30 µg/mL, respectively. Preliminary results showed that it is probable that our plant might act directly through the NO and guanylate cyclase signaling pathway and on muscarinic but not nicotinic receptors. The results reveal that the Essential oil of W. saharae appears to have an impact on intestinal relaxation in vitro conditions. This finding lends credence to the traditional usage of this plant to treat intestinal disorders.

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“…They have deterrent activities observed by many researchers (Thanigaivel et al, 2017;Hafsi et al, 2022). The interest in new botanical compounds for biological control is based on their bioefficiency, their biodegradability, their respect for the environment, their physiological activity (Baranitharan et al, 2020;Kharoubi et al, 2020;Zeghib et al, 2020;Rechek et al, 2021) and usually their low mammalian toxicity (El-Sabrout et al, 2020). They provide a safer option for controlling various types of insects (Sukumar et al, 1991).…”

Section: Introductionmentioning

confidence: 99%

Phytochemical composition of almond oil from Melia azedarach L. and its larvicidal, ovicidal, repellent and enzyme activities in Culex pipiens L.

et al. 2022

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Melia azedarach L. (Meliaceae) is a botanical species with focal point of global research for its biological properties. The Melia azedarach tree is distinguished by its rapid growth, its adaptation to different temperate zones, as well as its insecticidal properties. All this made us think of exploiting it in biological control against different stages of mosquitoes. To this end, we aim, through the present work, to evaluate the effectiveness of Melia azedarach extracts against Culex pipiens mosquito. More specifically, our study focuses on determining the chemical composition of Melia almond oil, as well as the larvicidal, ovicidal and repellent activities on Culex pipiens L. mosquito as well as the activities of acetylcholinesterase (AChE) and glutathione-S-transferase (GST). Almond oil was extracted by a Soxhlet and subjected to gas chromatography-mass spectrometry (GC/MS). The yield was found to be 35.17%. The chemical composition revealed the presence of various phytoconstituents. A total of 7 compounds were identified, the main ones being 9,11-Octadecadienoic acid, methyl ester, (E,E)-(79.32%), 9-octadecenoic acid (Z)-, methyl ester (13.24%), hexadecanoic acid and methyl ester (3.69%). The larvicidal bioassays were performed according to the protocol recommended by the World Health Organization with concentrations varying from 20 to 80 mg/L depending on the exposure time (24, 48 and 72 hours). The almond oil exhibited remarkable larvicidal activity against fourth instar larvae and the lethal concentrations were determined (LC 25 = 23.70 mg/L, LC 50 =35.49 mg/L, LC 90 =79.61 mg/L). The results also showed that the oil caused an ovicidal activity with a significant effect on egg hatch. The recorded hatching percentages were respectively 88.79% and 72.40% for the LC 25 and LC 50 , and this compared to the control series. Moreover, this oil exhibited significant repellency against adult mosquitoes. Furthermore, the enzymatic measurements performed on LC 50 and LC 90 treated larvae revealed a neurotoxic activity and a stimulation of the detoxification system as evidenced, respectively, by an inhibition of AChE and induction in GST activity. Overall, our data proved that Melia azedarach almond oil could be considered as a potent biorational alternative to synthetic insecticides for mosquito control.

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Chemical Composition and Antioxidant, Anti-Inflammatory, and Enzyme Inhibitory Activities of an Endemic Species from Southern Algeria: Warionia saharae

Cited by 10 publications

References 98 publications

Phlorotannins of the Brown Algae Sargassum vulgare from the Mediterranean Sea Coast

Phlorotannins of the Brown Algae Sargassum vulgare from the Mediterranean Sea Coast

The Antispasmodic Effect of Warionia saharae Essential Oil in Experimental Models and its Mechanism of Action

Phytochemical composition of almond oil from Melia azedarach L. and its larvicidal, ovicidal, repellent and enzyme activities in Culex pipiens L.

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