In Vitro Anti-Inflammatory and Anti-Diabetic Activity of Methanolic Extract of Cardanthera Difformis Druce

De, Somnath; Das, Debasish; Mandal, Tanusri

doi:10.7897/2230-8407.0712147

Cited by 7 publications

(4 citation statements)

References 14 publications

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“…The most commonly used drugs for the management of inflammatory conditions are non-steroidal anti-inflammatory drugs (NSAIDs). The greatest disadvantage in the presently available potent synthetic anti-inflammatory drugs lies in their toxicity, gastric irritation, thereby leading to the formation of gastric ulcers and the reappearance of symptoms after discontinuation [36]. Therefore, the search for natural bioactive compounds with anti-inflammatory activity has greatly increased in recent years.…”

Section: Resultsmentioning

confidence: 99%

Bioavailability and Bioactivity of Encapsulated Phenolics and Carotenoids Isolated from Red Pepper Waste

et al. 2019

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In order to deactivate the health properties of bioactive compounds, they need to withstand the effects of food processing, their potential release from the food matrix, and remain bio-accessible in the gastrointestinal tract. Bio-actives from different plants are prone to oxidative degradation, and encapsulation is an effective method in improving their stability. In the present study, red pepper waste (RPW), a by-product of vegetable processing industry, was encapsulated in whey protein using spray and freeze-drying techniques. The aim was to evaluate the effects of in vitro gastrointestinal digestion on the release and bioactivity of encapsulated bio-actives, after each digestion step. The results showed that the release of phenolics and carotenoids, as well as antioxidants, anti-hyperglycemic, and anti-inflammatory activities are influenced by pH and intestinal fluid, with pH 7.5 exhibited at higher levels. There was a rapid initial release of carotenoids from whey protein matrices, while a more gradual increase of phenolics was observed, reaching around 50% for both encapsulates first at 6 h and 37 °C, and small intestine conditions. The encapsulation of RPW demonstrated a protective effect against pH changes and enzymatic activities along digestion, and contributed to the increase in bio-accessibility in the gut. Also, the results suggest that encapsulation is an efficient method for valorization of bio-actives from RPW, with improvements in nutrition, color, and bioactive properties.

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Section: Resultsmentioning

confidence: 99%

Bioavailability and Bioactivity of Encapsulated Phenolics and Carotenoids Isolated from Red Pepper Waste

et al. 2019

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“…The percentage inhibition of protein denaturation was calculated relative to the control using the following formula [14] .…”

Section: Determination Of the Anti-inflammatory Activity Of Horsfeild...mentioning

confidence: 99%

Evaluation of In vitro anti-inflammatory activity and In-silico pharmacokinetics and molecular docking study of Horsfieldia iryaghedhi

Rajapaksha,

Fernando,

Nelumdeniya

et al. 2024

J Phytopharmacol

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Background: Phytochemicals are still a valuable source to develop clinically important drugs in treating chronic and acute diseases. Inflammation is a response to an injurious stimulus of the body and novel therapeutic agents are needed to alleviate the condition with minimum side effects. Aims and Objectives: To investigate in vitro anti-inflammatory activity of methanol and aqueous leaf, bark, and combination extracts of plant Horsfieldia iryaghedhi by heat-induced egg albumin denaturation method and to analyze the phytochemicals of Horsfieldia iryaghedhi for their anti-inflammatory potential against cyclooxygenase- 2 (COX-2) using molecular docking. Methodology: Matured and fully expanded fresh leaves and barks of H. iryaghedhi were collected, and the extractions were obtained cold maceration using 99.9% methanol and distilled water as solvents. A concentration series was then developed, and the anti-inflammatory activity was evaluated against Diclofenac sodium as the positive control, using the heat-induced egg albumin denaturation method. Further, selected phytochemicals were tested against COX-2 enzyme (PDB ID: 5IKR) using site-specific molecular docking with autodock vina and the binding energies and pharmacokinetic and toxicity parameters were evaluated. Results: The methanol and aqueous extracts have shown a moderate to strong concentration-dependent anti-inflammatory activity with reference to standard Diclofenac sodium (IC50 116.4 μg/ml) and Methanol bark extract exhibited potent anti-inflammatory activity compared to other extracts (IC50 293 μg/ml). Further, Methanol and aqueous extracts showed a statistically significant correlation between concentration and percentage inhibition (p<0.05, R2 ≈1). The molecular docking results suggest that the phytochemicals available on the plant have possible COX-2 inhibitory activity and the compounds selected (Methyl 2,4-dihydroxy-6-methylbenzoate and N, N-Dimethyl-5-methoxy tryptamine) even got favourable toxicity and pharmacokinetic parameters confirming their drugability. Conclusion: Methanol bark extract of Horsfieldia iryaghedhi have marked in vitro anti-inflammatory activity. The results indicate a solid possibility of lead discovery of anti- inflammatory agents from the bark and leaves of Horsfieldia iryaghedhi. However, further molecular dynamics studies and in vivo tests may be required to confirm the findings.

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“…After cooling the absorbance was measured at 660nm in UV-Vis spectrophotometer. The percentage inhibition of protein denaturation was calculated using the formula 6,7,8 % inhibition= (Vt/Vc-1)*100…”

Section: Extrudabilitymentioning

confidence: 99%

Formulation and Evaluation of Herbal Topical Gel Containing Leaves Extract of Andrographis paniculata

Samuel

Mulla

2020

J. Drug Delivery Ther.

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Objective: The present study has been undertaken with the aim to formulate and evaluate the gel contaning leaf extract of Andrographis Paniculata. Methodology and results: The formulation was designed by using alcoholic extract of leaves of Andrographis paniculata.The gel was prepared by using carbapol 934, triethanolamine, propylene glycol, methyl paraben, propyl paraben and required amount of distilled water. The prepared gel was evaluated for physical appearance, pH, spread ability, viscosity, extrudability, albumin denaturation assay and stability. Conclusion: Carbopol gels with dried leaves extract of Andrographis paniculata could be prepared successfully. Key words: Topical gel,Kalmegh, Spreadability, Albumin denaturation

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In Vitro Anti-Inflammatory and Anti-Diabetic Activity of Methanolic Extract of Cardanthera Difformis Druce

Cited by 7 publications

References 14 publications

Bioavailability and Bioactivity of Encapsulated Phenolics and Carotenoids Isolated from Red Pepper Waste

Bioavailability and Bioactivity of Encapsulated Phenolics and Carotenoids Isolated from Red Pepper Waste

Evaluation of In vitro anti-inflammatory activity and In-silico pharmacokinetics and molecular docking study of Horsfieldia iryaghedhi

Formulation and Evaluation of Herbal Topical Gel Containing Leaves Extract of Andrographis paniculata

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