In Vitro Anti-Biofilm Activity of Curcumin Nanoparticles in Acinetobacter baumannii: A Culture-Based and Molecular Approach

Hosseini, Atefeh; Nejadsattari, Taher; Zargar, Mohsen

doi:10.5812/archcid.83263

Cited by 9 publications

(5 citation statements)

References 29 publications

(31 reference statements)

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“…When Csu is inactivated , the ability of pili production and subsequently the ability of bacteria to format biofilms is inhibited 80 . Finally, we concluded that decreasing the expression of bap, OmpA, and csuA/B by AgNPs is a main approach for decreasing biofilm activity of A. baumannii that will help in reducing antibiotic resistance mechanisms 81 . The limitations of our study included lack of an in vivo study, which we plan to study in the future work.…”

Section: Discussionmentioning

confidence: 86%

Antibiofilm and antivirulence potential of silver nanoparticles against multidrug-resistant Acinetobacter baumannii

Hetta

Al-Kadmy

Khazaal

et al. 2021

Sci Rep

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We aimed to isolate Acinetobacter baumannii (A. baumannii) from wound infections, determine their resistance and virulence profile, and assess the impact of Silver nanoparticles (AgNPs) on the bacterial growth, virulence and biofilm-related gene expression. AgNPs were synthesized and characterized using TEM, XRD and FTIR spectroscopy. A. baumannii (n = 200) were isolated and identified. Resistance pattern was determined and virulence genes (afa/draBC, cnf1, cnf2, csgA, cvaC, fimH, fyuA, ibeA, iutA, kpsMT II, PAI, papC, PapG II, III, sfa/focDE and traT) were screened using PCR. Biofilm formation was evaluated using Microtiter plate method. Then, the antimicrobial activity of AgNPs was evaluated by the well-diffusion method, growth kinetics and MIC determination. Inhibition of biofilm formation and the ability to disperse biofilms in exposure to AgNPs were evaluated. The effect of AgNPs on the expression of virulence and biofilm-related genes (bap, OmpA, abaI, csuA/B, A1S_2091, A1S_1510, A1S_0690, A1S_0114) were estimated using QRT-PCR. In vitro infection model for analyzing the antibacterial activity of AgNPs was done using a co-culture infection model of A. baumannii with human fibroblast skin cell line HFF-1 or Vero cell lines. A. baumannii had high level of resistance to antibiotics. Most of the isolates harbored the fimH, afa/draBC, cnf1, csgA and cnf2, and the majority of A. baumannii produced strong biofilms. AgNPs inhibited the growth of A. baumannii efficiently with MIC ranging from 4 to 25 µg/ml. A. baumannii showed a reduced growth rate in the presence of AgNPs. The inhibitory activity and the anti-biofilm activity of AgNPs were more pronounced against the weak biofilm producers. Moreover, AgNPs decreased the expression of kpsMII , afa/draBC,bap, OmpA, and csuA/B genes. The in vitro infection model revealed a significant antibacterial activity of AgNPs against extracellular and intracellular A. baumannii. AgNPs highly interrupted bacterial multiplication and biofilm formation. AgNPs downregulated the transcription level of important virulence and biofilm-related genes. Our findings provide an additional step towards understanding the mechanisms by which sliver nanoparticles interfere with the microbial spread and persistence.

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Section: Discussionmentioning

confidence: 86%

Antibiofilm and antivirulence potential of silver nanoparticles against multidrug-resistant Acinetobacter baumannii

Hetta

Al-Kadmy

Khazaal

et al. 2021

Sci Rep

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“…The mechanism by which the process occurs is due to its ability to induce ROS. Curcumin demonstrated better antibiofilm activity than the drug nisidine and had an effect compared to that of chloramphenicol [116], with a proven antibiofilm effect on A. baumannii following in vitro tests [67,117]. A. baumannii is one of the most common microorganisms found in nosocomial and chronic infections, with a high resistance to antimicrobials and antibiotics [115][116][117][118].…”

Section: Methods Of Obtaining and Antiviral Antifungal And Antibacter...mentioning

confidence: 99%

“…Curcumin is known in traditional medicine, especially in India, for use in treating fever, skin infections, and to facilitate digestion due to its anti-inflammatory [6][7][8], antiviral , and antibacterial [63,117] properties. In the last two years, because of the COVID-19 pandemic, the congestion of hospitals has led to an increased level of nosocomial infections [136][137][138][139].…”

Section: Additive For Health Care Productsmentioning

confidence: 99%

Curcumin: Modern Applications for a Versatile Additive

Raduly

Rădiţoiu

et al. 2021

Coatings

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The recent development of several methods for extracting curcumin from the root of the plant Curcuma longa has led to intensified research on the properties of curcumin and its fields of application. Following the studies and the accreditation of curcumin as a natural compound with antifungal, antiviral, and antibacterial properties, new fields of application have been developed in two main directions—food and medical, respectively. This review paper aims to synthesize the fields of application of curcumin as an additive for the prevention of spoilage, safety, and quality of food. Simultaneously, it aims to present curcumin as an additive in products for the prevention of bacterial infections and health care. In both cases, the types of curcumin formulations in the form of (nano)emulsions, (nano)particles, or (nano)composites are presented, depending on the field and conditions of exploitation or their properties to be used. The diversity of composite materials that can be designed, depending on the purpose of use, leaves open the field of research on the conditioning of curcumin. Various biomaterials active from the antibacterial and antibiofilm point of view can be intuited in which curcumin acts as an additive that potentiates the activities of other compounds or has a synergistic activity with them.

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“…The interaction between negatively charged eDNA and positively charged AgNPs is an important factor in the inhibition of biofilms [184,185]. Additionally, selenium, curcumin, and aluminum oxide NPs were all found to inhibit the growth of A. baumannii biofilms [186][187][188].…”

Section: Nanoparticles (Nps)mentioning

confidence: 99%

Acinetobacter baumannii biofilm and its potential therapeutic targets

Mohamed,

Raafat,

Samir Mohamed

et al. 2023

Futur J Pharm Sci

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Background Acinetobacter baumannii (A. baumannii) is an opportunistic pathogen that poses dangerous health threat. It is a main cause of biofilm-associated infections that are mostly resistant to antibiotic therapy. Because of its capacity to form biofilm on biotic and abiotic surfaces, it has been linked to most nosocomial infections such as ventilator-associated pneumonia, urinary tract infections, bacteremia, meningitis, wound infections, soft tissue infections, and peritonitis. Main body of the abstract The biofilm refers to an organized group of microbial cells that are embedded in an exopolymeric substance made of protein, extracellular DNA, and polysaccharide. Bacterial cells in biofilms are resistant to chemicals, phagocytosis, and other elements of the body’s innate and acquired immune systems posing treatment challenges. Biofilm formation in A. baumannii is a complicated process that is influenced by a variety of factors such as outer membrane protein A, poly-β-(1,6)-N acetyl glucosamine (PAGE), biofilm-associated protein, two-component system (Bfm/S BfmR), chaperone–usher (Csu) pilus assembly system of pili, BlaPER-1 belonging to β-lactamase family, extracellular polymeric substance, and the quorum sensing system. Several biofilm-associated genes influence antimicrobial susceptibility, implying a link between biofilm formation and antimicrobial resistance. Short conclusion This review describes the complex biofilm system of A. baumannii, which gives it a survival advantage and increases its colonization ability. Also, it demonstrates various extrinsic and intrinsic factors that function and regulate the biofilm machinery of A. baumannii. Furthermore, this study considers prospective ways for preventing biofilm development on relevant medical equipment, as well as potential therapeutic strategies for eradicating mature biofilms, which can aid in the treatment of biofilm-associated A. baumannii infection.

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In Vitro Anti-Biofilm Activity of Curcumin Nanoparticles in Acinetobacter baumannii: A Culture-Based and Molecular Approach

Cited by 9 publications

References 29 publications

Antibiofilm and antivirulence potential of silver nanoparticles against multidrug-resistant Acinetobacter baumannii

Antibiofilm and antivirulence potential of silver nanoparticles against multidrug-resistant Acinetobacter baumannii

Curcumin: Modern Applications for a Versatile Additive

Acinetobacter baumannii biofilm and its potential therapeutic targets

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