In Vitro Anthelmintic Effect of some Medicinal Plants against Haemonchus Contortus

Akther, Sonia; Dey, Anita Rani; Hossain, Shahadat; Dey, Taposhi Rani; Begum, Nurjahan

doi:10.5455/jasa.20141230103624

Cited by 4 publications

(7 citation statements)

References 8 publications

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“…However, KIE showed a significant anthelmintic activity against larval development, infective larvae (L3), and the adults. Similar results as in treatment P3, P4, and P8 were reported by Akhter et al (2015) which studied the effect of infuse (10%) and maceration (5%) extracts of ofneem, tamak, korola flower, chatim, and sharma on infective larvae and the adults of Haemonchus contortus. However, Table 3.…”

Section: Ovicidal Larvicidal and Nematicidal Activity Of Kemuning Lsupporting

confidence: 86%

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Phytochemical Screening and in Vitro Ovicidal, Larvacidal, and Nematicidal Effects of Murraya paniculata (L.) Jack Extract on Gastrointestinal Parasites of Goats

Tresia¹,

Evvyernie²,

Tiuria³

2016

Med Pet

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In our previous research, kemuning leaves (Murraya paniculata L. Jack) was shown to have the capability as an anthelmintic candidate for PE (Ettawa crossbred) lactating dairy goats by reducing 43.67% of the egg per gram (EPG) of Strongylida compared to 0.0005% in orally treated with Oxfendazole as a control. To confirm it, the aim of this in vitro study was to determine the effective dosage of kemuning leaves from two extraction methods (infuse and maceration) to reduce the Trichostrongylidae and to evaluate the bioactive compounds of the leaves. The research was conducted using a randomized complete design with 11 treatments and 5 replications. The treatments consisted of control (0.0005% and 0.005% Oxfendazole), kemuning leaves infuse extract (KIE) and maceration extract (KME) each with level of 1%, 3%, 5%, and 7% (w/v). The results showed that the LT 50 gradually decreased (shortening the lethal time) and the mortality of Trichostrongylidae gradually increased associated with the increased concentration of treatment (P<0.01). The infusion of 7% kemuning extract demonstrated the highest efficiency in reducing the larval development, infective larvae, and the adult Trichostrongylidae by 93.16%, 94.39%, and 90%, respectively. This treatment could be developed as the most prospective herbal anthelmintic drug in controlling the infection by Trichostrongylidae.

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Section: Ovicidal Larvicidal and Nematicidal Activity Of Kemuning Lsupporting

confidence: 86%

“…Feces (300 g) were fertilized according to Akhter et al (2015) and the larvae were harvested following modified Baermann larval collection technique (1917). The larvae-containing liquid (0.01 mL) was transferred into counting plate (diameter= 2 cm) and added with treatments.…”

Section: Larvicidal Assay (Akhter Et Al 2015)mentioning

confidence: 99%

Phytochemical Screening and in Vitro Ovicidal, Larvacidal, and Nematicidal Effects of Murraya paniculata (L.) Jack Extract on Gastrointestinal Parasites of Goats

Tresia¹,

Evvyernie²,

Tiuria³

2016

Med Pet

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“…The eggs were recovered from suspension by the method described by Coles et al (1992). These eggs were cultured at room temperature in damp heatsterilized bovine faeces for 7 days to provide development using the method of Makun et al (2008) and Dey et al (2015). After 7 days, the culture was baermannized to harvest the L 3 stages and stored in distilled water at 4°C in the laboratory.…”

Section: Sourcing Of Infective Larvae and Adult Stage Of H Contortus ...mentioning

confidence: 99%

“…Among the helminth parasites that infect small ruminants, Haemonchus contortus is said to be considered the most devastating and prevalent species (Dey et al, 2015). This has led to a major constraint in the production and profitability of small ruminant in Nigeria and African at large (Chiejina, 2001).…”

Section: Introductionmentioning

confidence: 99%

In vitro anthelmintic activities of stem and root barks extracts of Parkia biglobosa on infective larvae and adult of Haemonchus contortus

Josiah¹,

Adama²,

Jiya³

et al. 2023

Afr. J. Biotechnol.

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The treatment of gastrointestinal nematode infections in the 21st century is largely through the use of modern synthetic anthelmintics. However, there is over growing drug resistance to anthelmintics in treatment of gastrointestinal nematode infections in goats and sheep. This present study was carried out to determine the in vitro anthelmintic activitities of Parkia biglobosa using Larval motility inhibition assay (LMIA) and Adult motility inhibition assay (AMIA). The stem and root barks of P. biglobosa were extracted which yielded four different extracts as Crude Aqueous Stem Bark Extract (CASBE), Crude Methanol Stem Bark Extract (CMSBE), Crude Aqueous Root Bark Extract (CARBE) and Crude Methanol Root Bark Extract (CMRBE). The infective larvae and adults of Haemonchus contortus were exposed to different concentrations each (2, 4, 8. 16 and 32 mg/ml) of plant extracts of both stem and root barks of P. biglobosa in comparable to controls (Albendazole (ABZ)-positive control and Phosphate Buffered Saline (PBS)-negative control). The result of LMIA showed that at 12 hours exposure of larvae to 32 mg/ml for four extracts, 55-75% mortality were recorded while the result for AMIA revealed that at 12 hours post exposure of all the plant extracts ranged from 2-32 mg/ml concentrations, 100% mortality of the adult worms were recorded. There was no mortality recorded in negative control (PBS) even up to 12 hours post exposure. From this result, it could be concluded that plant extracts have anthelmintic activities in comparison to ABZ with CMSBE ranked highest among the extracts. However, the potency of plant extracts was dependent on the time of exposure and concentration of the extracts as well as the solvent used.

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“…They are considered to be the most prevalent and devastating species, thriving mostly in warm and humid areas. The death rate due to haemonchosis is very high and may be up to 50 % in some small ruminant communities [10].…”

Section: Introductionmentioning

confidence: 99%

Anthelmintic Activity ofHymenodictyon pachyantaStem Bark Extracts against Haemonchus Contortus

Olayemi¹,

Onakpa

Jegede³

2019

Folia Veterinaria

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The development of host resistance to anthelmintics and the increasing cost of commercial anthelmintics have encouraged the need for the in vitro anthelmintic evaluation of crude extract and fractions of Hymenodictyon pachyanta plant as alternative drugs against Haemonchus contortus. H. contortus is one of the most prevalent and highly pathogenic parasitic nematodes in small ruminant farming globally. H. pachyanta stem bark is a prospective plant used by the local and indigenous farmers of Nsukka, Enugu state, Nigeria. The stem bark of H. pachyanta were collected, dried, pulverized and extracted with 80 % methanol. The purpose of this study was to investigate the in vitro anthelmintic effects of these crude extract and fractions against H. contortus in sheep and goats. The two extracts (crude and fractions) of H. pachyanta were tested by the egg hatch assay (EHA) and the larval development inhibition assays (LDIA) and to compared the results with albendazole (as the positive control). The concentrations for the crude extract and albendazole used for this study were 0.78, 1.56, 3.125, 6.25 and 12.5 mg.ml−1. The results demonstrated that the crude extracts, fractions and albendazole all at the concentration doses of 12.5 mg.ml−1 produced 100 % inhibition of egg hatching and larval development. Statistically, there was no significant difference (P > 0.05) in the mean percentage inhibition of egg hatching and larval development inhibition of the crude extracts and fractions when compared with albendazole. However, a significant difference (P < 0.05) was observed with n-butanol fraction which inhibited 96.17 % of egg hatchability. All of the extracts and albendazole showed ovicidal and larvicidal effects and were able to induce over 50 % of the egg hatching and mortality of larvae at the concentration ranges of 0.78—12.5 mg.ml−1. The results obtained from our study suggest that H. pachyanta had ovicidal and larvicidal activity against H. contortus and that the bioactive plants compounds responsible for this effect could be attributed to the presence of tannins, alkaloids and the saponins contained in the crude extracts.

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In Vitro Anthelmintic Effect of some Medicinal Plants against Haemonchus Contortus

Cited by 4 publications

References 8 publications

Phytochemical Screening and in Vitro Ovicidal, Larvacidal, and Nematicidal Effects of Murraya paniculata (L.) Jack Extract on Gastrointestinal Parasites of Goats

Phytochemical Screening and in Vitro Ovicidal, Larvacidal, and Nematicidal Effects of Murraya paniculata (L.) Jack Extract on Gastrointestinal Parasites of Goats

In vitro anthelmintic activities of stem and root barks extracts of Parkia biglobosa on infective larvae and adult of Haemonchus contortus

Anthelmintic Activity ofHymenodictyon pachyantaStem Bark Extracts against Haemonchus Contortus

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