In vitro anthelmintic activity of five tropical legumes on the exsheathment and motility of Haemonchus contortus infective larvae

Fernex, Elke von Son-de; Alonso-Díaz, Miguel Ángel; Mora, Braulio Valles de la; Capetillo-Leal, C.M.

doi:10.1016/j.exppara.2012.05.010

Cited by 33 publications

(16 citation statements)

References 26 publications

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“…Oliveira et al [ 53 ], showed the inhibition of larval exsheathment in H. contortus subject to A. mangium extract. Son-de Fernex et al [ 64 ] observed an inhibition effect on exsheathment of H. contortus larvae subjected to five plants containing CTs. Consequently, we think that other metabolites besides tannins would be able to affect the exsheathment kinetics of L 3 larvae by binding to proteins and glycoproteins of the sheath to prevent the formation of the indented ring in the anterior part.…”

Section: Discussionmentioning

confidence: 99%

Comparison of thein vitroanthelmintic effects ofAcacia niloticaandAcacia raddiana

et al. 2017

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Gastrointestinal nematodes are a major threat to small ruminant rearing in the Sahel area, where farmers traditionally use bioactive plants to control these worms, including Acacia nilotica and Acacia raddiana. The main aim of this study was to screen the potential anthelmintic properties of aqueous and acetone extracts of leaves of these two plants based on three in vitro assays: (1) the egg hatch inhibition assay (EHA); (2) the larvae exsheathment inhibition assay (LEIA) using Haemonchus contortus as a model; and (3) an adult mortality test (AMT) applied on Caenorhabditis elegans. For the EHA, only A. raddiana was effective with IC50 = 1.58 mg/mL for aqueous extract, and IC50 = 0.58 mg/mL for acetonic extract. For the LEIA, all extracts inhibited the exsheathment of larvae compared to the controls, and the aqueous extract of A. nilotica was more larvicidal with IC50 = 0.195 mg/mL. In general, all responses to the substances were dose-dependent and were significantly different from the control group (p < 0.05). For the AMT, the extracts of the two Acacia species were effective but A. raddiana showed greater efficacy with 100% mortality at 2.5 mg/mL and LC50 = 0.84 mg/mL (acetonic extract). The addition of polyvinyl polypyrrolidone (PVPP) to the extracts suggested that tannins were responsible for blocking egg eclosion and inducing adult mortality but were not responsible for exsheathment inhibition. These results suggest that the leaves of these Acacia species possess ovicidal and larvicidal activities in vitro against H. contortus, and adulticidal effects against C. elegans.

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Section: Discussionmentioning

confidence: 99%

Comparison of thein vitroanthelmintic effects ofAcacia niloticaandAcacia raddiana

et al. 2017

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“…Secondary metabolites such as alkaloids, terpenes, tannins or flavonoids contained in crude plant extracts have been related to parasiticidal activity [32-35]. Nevertheless, since these are not the only compounds that these and other plant species possess, it would be wrong to discard the effect of other bioactive compounds.…”

Section: Discussionmentioning

confidence: 99%

In vitro antihelmintic effect of fifteen tropical plant extracts on excysted flukes of Fasciola hepatica

et al. 2015

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BackgroundFasciolosis due to Fasciola hepatica is the most important hepatic disease in veterinary medicine. Its relevance is important because of the major economical losses to the cattle industry such as: reduction in milk, meat and wool production; miscarriages, anemia, liver condemnation and occasionally deaths, are estimated in billons of dollars.The emergence of fluke resistance due to over or under dosing of fasciolides as well as environmental damage produced by the chemicals eliminated in field have stimulated the need for alternative methods to control Fasciola hepatica. The aim of this study was to evaluate the in vitro anthelmintic effect of fifteen tropical plant extracts used in tradicional Mexican medicine, on newly excysted flukes of Fasciola hepatica.ResultsThe flukes were exposed in triplicate at 500, 250 and 125 mg/L to each extract. The efficacy was assessed as the mortality rate based on the number of live and dead flukes after 24, 48 and 72 h post-exposure. The plants with anthelmintic effect were evaluated once again with a concentration of 375 mg/L in order to confirm the results and to calculate lethal concentrations at 50%, 90% and 99% (LC50, LC90, and LC99). Plant extracts of Lantana camara, Bocconia frutescens, Piper auritum, Artemisia mexicana and Cajanus cajan had an in vitro anthelmintic effect (P <0.05). The LC50, LC90 and LC99 to A. mexicana, C. cajan and B. frutescens were 92.85, 210.44 and 410.04 mg/L, 382.73, 570.09 and 788.9 mg/L and 369.96, 529.94 and 710.34 mg/L, respectively.ConclusionIt is concluded that five tropical plant extracts had promising anthelmintic effects against F. hepatica. Further studies on toxicity and in vivo biological evaluation in ruminant models might help to determine the anthelmintic potential of these plant extracts.

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“…4,8 Phenols, such as tannins, may negatively impact nematode egg hatching by binding to the egg cuticle which is rich in glycoproteins, forming a tannin-protein complex that may alter embryo development and eventually the hatching process. 25,26 Parasitic load in H. contortus infected hair sheep was decreased when animals were fed with leaves of the L. acapulcensis tree, with an added beneficial effect in productive performance. 27 Authors found a high content of condensed tannins (116 g/kg of dry matter) in these leaves.…”

Section: Egg Hatching Inhibitionmentioning

confidence: 99%

Caesalpinia coriaria fruits and leaves extracts possess in vitro ovicidal activity against Haemonchus contortus and Haemonchus placei

et al. 2019

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The ovicidal effect of hydro-alcoholic extracts from mature fruits and dried leaves of the Caesalpinia coriariatree, against Haemonchus contortus and H. placei was evaluated by the egg hatching inhibition test (EHIT). Secondary compounds within the extracts were identified by high performance liquid chromatography. Five extract concentrations were assessed in the inhibition tests (25.0, 12.5, 6.2, 3.1 and 1.5 mg/mL), using distilled water and Thiabendazole (0.5 mg/mL) as negative and positive controls, respectively. The results were analysed under a 2 × 2 × 6 completely randomized factorial design, and mean differences were established by the Tukey’s post hoc test. The 50% (EC50) and 90% (EC90) effective concentrations for each extract were determined using a Probit analysis.A concentration-dependent ovicidal effect of hydro-alcoholic extracts from both leaves and fruits was observed, reaching a 100% efficacy against both nematode species with the 25.0 mg/mL concentration (p < 0.05). The EC50 for the fruit and leaves extracts were established as 1.63 and 3.91 mg/mL, and as 3.98 and 11.68 mg/mL, against H. contortus and H. placei, respectively. The most important secondary compounds identified in extracts were free-condensed tannins, gallic acid and methyl-gallate.In conclusion,hydro-alcoholic extracts from C. coriaria mature fruits and dried leaves inhibit in vitro egg hatching of H. contortus and H. placei.

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In vitro anthelmintic activity of five tropical legumes on the exsheathment and motility of Haemonchus contortus infective larvae

Cited by 33 publications

References 26 publications

Comparison of thein vitroanthelmintic effects ofAcacia niloticaandAcacia raddiana

Comparison of thein vitroanthelmintic effects ofAcacia niloticaandAcacia raddiana

In vitro antihelmintic effect of fifteen tropical plant extracts on excysted flukes of Fasciola hepatica

<em>Caesalpinia coriaria</em> fruits and leaves extracts possess <em>in vitro</em> ovicidal activity against <em>Haemonchus contortus</em> and <em>Haemonchus placei</em>

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