In Vitro and In Vivo Study of a Novel Porcine Collagen Membrane for Guided Bone Regeneration

Abstract

For years, in order to improve bone regeneration and prevent the need of a second stage surgery to remove non-resorbable membranes, biological absorbable membranes have gradually been developed and applied in guided tissue regeneration (GTR). The present study’s main objective was to achieve space maintenance and bone regeneration using a new freeze-dried developed porcine collagen membrane, and compare it with an already commercial collagen membrane, when both were used with a bovine xenograft in prepared alveolar ridge bone defects. Prior to surgery, the membrane’s vitality analysis showed statistically significant higher cell proliferation in the test membrane over the commercial one. In six beagle dogs, commercial bone xenograft was packed in lateral ridge bone defects prepared in the left and right side and then covered with test porcine collagen membrane or commercial collagen membrane. Alveolar height changes were measured. Histomorphometric results, in vitro and in vivo properties indicated that the new porcine collagen membrane is biocompatible, enhances bone xenograft osteoconduction, and reduces the alveolar ridge height reabsorption rate.

Osteopromotion Capacity of Bovine Cortical Membranes in Critical Defects of Rat Calvaria: Histological and Immunohistochemical Analysis

“…In humans, a 50% reduction in the horizontal dimension is evident up to 12 months after the tooth extraction and the most expressive loss occurs in the first 3 months of healing [3]. e increased demand for dental implant treatments in the 1980s spurred the development and refinement of surgical techniques for bone grafting, including alveolar ridge preservation procedures [4], alveolar distraction, and guided bone regeneration (GBR) [5,6]. GBR is based on the use of resorbable or nonresorbable barrier membranes that prevent the migration of certain types of cells into the bone defect area, such as rapidly growing epithelial cells and connective tissue, favoring the proliferation of osteoprogenitor cells that are able to perform bone neoformation [5,6].…”

Platelet‐rich fibrin and collagen membrane in the preservation of the alveolar bone: Feasibility of the elemental inorganic composition and scanning electron microscopy analysis

“…In the 1980s, the use of a nonabsorbable expanded polytetrafluoroethylene membrane for RP able to barricade the downgrowth of gingival epithelial cells and create a space for new bone formation began in earnest (Nyman, Gottlow, Karring, & Lindhe, ; Salamanca et al, ). Despite the various advantages of nonresorbable membranes, their association with a higher risk of membrane exposure or wound dehiscence has reduced their clinical usage compared to resorbable membranes (Park, Choi, Cho, & Lee, ).…”

“…The advantageous properties of collagen membranes include increasing hemostatic function, allowing early wound stabilization; its hemostatic properties, attracting fibroblasts; and its semipermeability, facilitating nutrient transfer. A major drawback of native collagen is its biodegradation resulting from the enzymatic activity of macrophages and leukocytes (Park et al, ; Salamanca et al, ). In RP, these membranes are effective at minimizing alveolar ridge shrinkage and producing favorable histologic results, enabling the subsequent and successful placement of an endo‐osseous dental implant.…”

Membranas de quitosana/gelatina com nanopartículas de prata para regeneração tecidual

“…Média, assim como de dentes artificiais feitos de conchas pelos maias (600 AC), de ferro pelos franceses (200 AC) e de ouro e madeira pelos romanos, chineses e astecas (Pires et al, 2015). Nos últimos anos, cerâmicas, metais e materiais poliméricos têm sido usados para substituir ou reparar diferentes partes do corpo (Hench, 1998 (Salamanca et al, 2016). Estes curativos são muitas vezes referidos coletivamente como "retentores de umidade" ou "semi-oclusivos" (Ovington, 2007) (Itoh et al, 2001;Salamanca et al, 2016), gelatina (G), que como um derivado desnaturado particular do colágeno, tem excelente biocompatibilidade e é biodegradável (Li et al, 2007;Bubalo et al, 2012;Salamanca et al, 2016).…”

“…Nos últimos anos, cerâmicas, metais e materiais poliméricos têm sido usados para substituir ou reparar diferentes partes do corpo (Hench, 1998 (Salamanca et al, 2016). Estes curativos são muitas vezes referidos coletivamente como "retentores de umidade" ou "semi-oclusivos" (Ovington, 2007) (Itoh et al, 2001;Salamanca et al, 2016), gelatina (G), que como um derivado desnaturado particular do colágeno, tem excelente biocompatibilidade e é biodegradável (Li et al, 2007;Bubalo et al, 2012;Salamanca et al, 2016). Neste contexto, sabemos que membranas devem possuir requisitos indispensáveis para agir como barreira física passiva: biocompatibilidade, propriedades oclusivas, capacidade de criação de espaço, integração tecidual e facilidade de uso (Costa et al, 2016)..…”