In vitro and in vivo anti-oxidation and anti-fatigue effect of monkfish liver hydrolysate

Xu, Jiajie; Li, Yanyan; Regenstein, Joe M.; Su, Xiurong

doi:10.1016/j.fbio.2017.03.002

Cited by 34 publications

(18 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The fish peptide‐treated rats had levels of CAT (283.6 U/min/mg of protein), glutathione‐S‐transferase (4.3 U/min/mg of protein) and SOD (28.42 U/min/mg of protein) that were significantly higher than the 196.4, 1.3, and 15.1, respectively, for the negative control. A fish liver protein hydrolyzate with strong DPPH radical scavenging ability displayed anti‐fatigue bioactive effects when fed to mice (Xu, Li, Regenstein, & Su, ). After 6 weeks of consuming the hydrolyzate, there was a dose‐dependent significant increase in longset climbing time of the mice.…”

Section: Antioxidant Propertiesmentioning

confidence: 99%

“…Although the ability of protein hydrolyzates and bioactive peptides to act as antioxidative agents has been roundly demonstrated during in vitro (Agrawal, Joshi, & Gupta, ; Alashi et al, ; Harnedy et al, ; Lin et al, ; Rahman et al, ; Wong et al, ) and in vivo (Girgih, Alashi et al, ; Liu et al, ; Mada et al, ; Xu, Li et al, ; Zhao et al, ) tests, there is a dearth of human clinical trials replicating the results. Given the challenge often encountered in translating results from animal and chemical‐based assays to clinical outcomes, future work should be designed to include tests in human subjects.…”

Section: Future Research Needsmentioning

confidence: 99%

See 1 more Smart Citation

Structural and functional properties of food protein-derived antioxidant peptides

2019

View full text Add to dashboard Cite

The aim of this work is to provide a timely examination of the structure-activity relationship of antioxidative peptides. The main production approach involves enzymatic hydrolysis of animal and plant proteins to produce protein hydrolyzates, which can be further processed by membrane ultrafiltration into size-based peptide fractions. The hydrolyzates and peptide fractions can also be subjected to separation by column chromatography to obtain pure peptides. Although the structural basis for enhanced antioxidant activity varies, protein hydrolyzates and peptide fractions that contain largely low molecular weight peptides have generally been shown to be potent antioxidants. In addition to having hydrophobic amino acids such as Leu or Val in their N-terminal regions, protein hydrolyzates, and peptides containing the nucleophilic sulfur-containing amino acid residues (Cys and Met), aromatic amino acid residues (Phe, Trp, and Tyr) and/or the imidazole ring-containing His have been generally found to possess strong antioxidant properties. Practical applicationsHigh levels of reactive oxygen species (ROS) in addition to the presence of metal cations can lead to oxidative stress, which promotes reactions that cause destruction of critical cellular biopolymers, such as proteins, lipids, and nucleic acids. Oxidative stress could be due to insufficient levels of natural cellular antioxidants, which enables accumulation of ROS to toxic levels. A proposed approach to ameliorating oxidative stress is the provision of exogenous peptides that can be consumed to complement cellular antioxidants. Food protein-derived peptides consist of amino acids joined by peptides bonds just like glutathione, a very powerful natural cellular antioxidant. Therefore, this review provides a timely summary of the in vitro and in vivo reactions impacted by antioxidant peptides and the postulated mechanisms of action, which could aid development of potent antioxidant agents. The review also serves as a resource material for identifying novel antioxidant peptide sources for the formulation of functional foods and nutraceuticals. K E Y W O R D S antioxidants, FRAP, lipid peroxidation, metal chelation, peptides, protein hydrolyzates, radical scavenging [Correction added on 30 January 2019: this article has been added to the issue after an inadvertent omission.]

show abstract

Section: Antioxidant Propertiesmentioning

confidence: 99%

Section: Future Research Needsmentioning

confidence: 99%

Structural and functional properties of food protein-derived antioxidant peptides

2019

View full text Add to dashboard Cite

show abstract

“…Several recent studies have described the antioxidant properties of FPH of muscle and liver of Lophius litulon species [ 19 , 20 , 21 ]. In monkfish flesh, specific peptides with high antioxidant activities were isolated and suggested as additives to delay lipid peroxidation in foodstuffs [ 19 ], as well as being potential hepatoprotective agents [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

Optimal Production of Protein Hydrolysates from Monkfish By-Products: Chemical Features and Associated Biological Activities

et al. 2020

View full text Add to dashboard Cite

The aim of this work was the recovery of protein substrates from monkfish waste (heads and viscera) generated in the on-board processing of this species. Initially, the effect of pH, temperature, and protease concentration was studied on mixtures of a 1:1 ratio (w/v) of monkfish heads/water. The optimal conditions of proteolytic digestion were established at 57.4 °C, pH 8.31, [Alcalase] = 0.05% (v/w) for 3 h of hydrolysis. Later on, a set of hydrolysis at 5L-pH-stat reactor were run under the aforementioned conditions, confirming the validity of the optimization studies for the head and viscera of monkfish. Regarding the chemical properties of the fish protein hydrolysates (FPH), the yield of digestion was higher than 90% in both cases and the degrees of hydrolysis and the soluble protein content were not especially large (<20% and <45 g/L, respectively). In vitro digestibility was higher than 90% and the percentage of essential amino acids ranged from 40 to 42%. Antioxidant activities were higher in viscera FPH, and antihypertensive ability was superior in head FPH. The values of number average molecular weights (Mn) of monkfish hydrolysates were 600 Da in the viscera and 947 Da in the head. The peptide size distribution, obtained by size-exclusion chromatography, indicated that the largest presence of peptides below 1000 Da and 200 Da was observed in the viscera FPH.

show abstract

“…Vitamin C was used as a positive reference. All experiments were in triplicate [18]. e ability to scavenge DPPH radicals was calculated by the following formula:…”

Section: Measurement Of Antioxidant Enzymes and Lipid Peroxidation Inmentioning

confidence: 99%

Antifatigue Activity of Glycoprotein from Schisandra chinensis Functions by Reducing Oxidative Stress

Shao

Wang

Zhang

et al. 2020

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

The glycoprotein from Schisandra chinensis was obtained with alkali extraction and acid precipitation, purified with DEAE Sepharose Fast Flow and Superdex G-75 column. The molecular composition structure and antifatigue activities of glycoprotein were studied. SCGP’s molecular weight was approximately 10 KDa, and it consisted of a carbohydrate component (52.94%) and protein component (47.06%). SCGP comprised mannose, galactoside, rhamnose, glucose, galactose, xylose, arabinose, and fucose, its molar ratio was 2.14 : 1.43 : 1.59 : 8.17 : 8.99 : 3.18 : 18.51 : 1, and it contained 16 kinds of amino acids. SCGP could obviously extend the swimming time in mice by increasing LDH, SOD level, GSH-Px activity, and liver glycogen and decreasing the contents of BUN and MDA. The antioxidant activity of SCGP is a potential mechanism of its antifatigue effect. In vitro antioxidant test showed that SCGP scavenged DPPH and OH radicals in a dose-dependent manner (IC50 was 0.91 mg/ml and 0.72 mg/ml).

show abstract

In vitro and in vivo anti-oxidation and anti-fatigue effect of monkfish liver hydrolysate

Cited by 34 publications

References 59 publications

Structural and functional properties of food protein-derived antioxidant peptides

Structural and functional properties of food protein-derived antioxidant peptides

Optimal Production of Protein Hydrolysates from Monkfish By-Products: Chemical Features and Associated Biological Activities

Antifatigue Activity of Glycoprotein from Schisandra chinensis Functions by Reducing Oxidative Stress

Contact Info

Product

Resources

About