In vitro and in vivo evaluation of a novel nanosize hydroxyapatite particles/poly(ester-urethane) composite scaffold for bone tissue engineering

Laschke, Matthias W.; Strohe, A.; Menger, Michael D.; Alini, Mauro; Eglin, David

doi:10.1016/j.actbio.2009.12.004

Cited by 125 publications

(99 citation statements)

References 32 publications

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“…Notably, it was found that the initial vascularisation of the scaffolds is crucially dependent on their biocompatibility. For instance, polyurethane scaffolds, which do not induce a leukocytic infl ammatory host tissue response, are only poorly vascularised throughout the fi rst 14 days after implantation (Laschke et al, 2009c(Laschke et al, , 2010a. In contrast, PLGA scaffolds, which induce a www.ecmjournal.org MW Laschke et al Biomaterials research with the skinfold chamber slight infl ammation, show a strong angiogenic reaction .…”

Section: Scaffolds For Tissue Engineeringmentioning

confidence: 99%

“…For instance, the analysis of venular leukocyte-endothelial cell interaction in tumour necrosis factor (TNF)-α-treated dorsal skinfold chamber preparations of mice defi cient in angiopoietin (Ang)-2 could identify Ang-2 as an important autocrine regulator of endothelial cell inflammatory responses (Fiedler et al, 2006, additional reference). Moreover, intravital fl uorescence microscopy of in situ prevascularised scaffolds from green fl uorescent protein (GFP)-transgenic mice implanted into dorsal skinfold chambers of wild-type recipient animals has been used to study the interaction of the implants' preformed blood vessels with the host microvasculature at the implantation site (Laschke et al, , 2011. Based on the fi ndings of such studies it may be possible in the future to develop novel biomaterial implants with an improved biocompatibility and vascularisation potential.…”

Section: Mw Laschke Et Al Biomaterials Research With the Skinfold Chmentioning

confidence: 99%

“…First studies provide evidence that this may be achieved by in vitro short-term cultivation of in situ prevascularised scaffolds. This short-term cultivation results in the destabilisation of their preformed microvascular networks (Laschke et al, 2011), which represents an essential step in the process of angiogenesis. Moreover, the outgrowth of vessel sprouts from prevascularised scaffolds may be promoted by embedding them in an extracellular pro-angiogenic matrix, resulting in an improved external inosculation in the surrounding host tissue after implantation (Laschke et al, 2010b).…”

Section: Mw Laschke Et Al Biomaterials Research With the Skinfold Chmentioning

confidence: 99%

“…During the last few years, various scaffold types have been evaluated in terms of biocompatibility and vascularisation in dorsal skinfold chambers of mice. These scaffolds consisted of collagen (Ichioka et al, 2005), collagen-elastin (Ring et al, 2010a), collagen-chitosanhydroxyapatite hydrogel , acellular dentin (Rücker et al, 2008), hydroxyapatite (Rücker et al, 2008), β-tricalcium-phosphate , poly(ether ester) (Druecke et al, 2004), poly(L-lactideco-glycolide) (PLGA) , polyethylene glycol terephthalate/polybutylene terephthalate (PEGT/ PBT) (Ring et al, 2006a,b), lactocapromer terpolymer (Ring et al, 2010b(Ring et al, , 2011b, polyurethane (Laschke et al, 2009c(Laschke et al, , 2010a and supramolecular nanofi bres (Ghanaati et al, 2009). Notably, it was found that the initial vascularisation of the scaffolds is crucially dependent on their biocompatibility.…”

Section: Scaffolds For Tissue Engineeringmentioning

confidence: 99%

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The dorsal skinfold chamber: window into the dynamic interaction of biomaterials with their surrounding host tissue

Laschke

Vollmar²,

Menger³

2011

eCM

143

128

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The implantation of biomaterials into the human body has become an indispensable part of almost all fi elds of modern medicine. Accordingly, there is an increasing need for appropriate approaches, which can be used to evaluate the suitability of different biomaterials for distinct clinical indications. The dorsal skinfold chamber is a sophisticated experimental model, which has been proven to be extremely valuable for the systematic in vivo analysis of the dynamic interaction of small biomaterial implants with the surrounding host tissue in rats, hamsters and mice. By means of intravital fl uorescence microscopy, this chronic model allows for repeated analyses of various cellular, molecular and microvascular mechanisms, which are involved in the early infl ammatory and angiogenic host tissue response to biomaterials during the initial 2-3 weeks after implantation. Therefore, the dorsal skinfold chamber has been broadly used during the last two decades to assess the in vivo performance of prosthetic vascular grafts, metallic implants, surgical meshes, bone substitutes, scaffolds for tissue engineering, as well as for locally or systemically applied drug delivery systems. These studies have contributed to identify basic material properties determining the biocompatibility of the implants and vascular ingrowth into their surface or internal structures. Thus, the dorsal skinfold chamber model does not only provide deep insights into the complex interactions of biomaterials with the surrounding soft tissues of the host but also represents an important tool for the future development of novel biomaterials aiming at an optimisation of their biofunctionality in clinical practice.

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Section: Scaffolds For Tissue Engineeringmentioning

confidence: 99%

Section: Mw Laschke Et Al Biomaterials Research With the Skinfold Chmentioning

confidence: 99%

Section: Mw Laschke Et Al Biomaterials Research With the Skinfold Chmentioning

confidence: 99%

Section: Scaffolds For Tissue Engineeringmentioning

confidence: 99%

See 2 more Smart Citations

The dorsal skinfold chamber: window into the dynamic interaction of biomaterials with their surrounding host tissue

Laschke

Vollmar²,

Menger³

2011

eCM

143

128

View full text Add to dashboard Cite

show abstract

“…Polyesters are the most used polymers in orthopedics. As polymers have converse mechanical properties to ceramics, interesting composites have been proposed based on the association of both [49]. Synthetic scaffolds can be coated with extracellular bone matrix proteins as well as seeded with mesenchymal stem cells.…”

Section: Artificial Graftsmentioning

confidence: 99%

Updates in biological therapies for knee injuries: bone

Kfuri

Freitas

Batista

et al. 2014

Curr Rev Musculoskelet Med

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Bone is a unique tissue because of its mechanical properties, ability for self-repair, and enrollment in different metabolic processes such as calcium homeostasis and hematopoietic cell production. Bone barely tolerates deformation and tends to fail when overloaded. Fracture healing is a complex process that in particular cases is impaired. Osteoprogenitor cells proliferation, growth factors, and a sound tridimensional scaffold at fracture site are key elements for new bone formation and deposition. Mechanical stability and ample vascularity are also of great importance on providing a proper environment for bone healing. From mesenchymal stem cells delivery to custom-made synthetic scaffolds, many are the biological attempts to enhance bone healing. Impaired fracture healing represents a real burden to contemporary society. Sound basic science knowledge has contributed to newer approaches aimed to accelerate and improve the quality of bone healing.

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