In vitro and in vivo evaluation of anti-cancer activity: Shape-dependent properties of TiO 2 nanostructures

Latha, T. Sree; Reddy, Madhava C.; Muthukonda, Shankar V.; Srikanth, Vadali V. S. S.; Lomada, Dakshayani

doi:10.1016/j.msec.2017.04.011

Cited by 33 publications

(18 citation statements)

References 43 publications

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“…It has been explicitly found that the highest protein kinase inhibition activity was demonstrated by N-doped TiO 2 nanoparticles in comparison to their bare counterpart which might be due to the enhanced surface area of TiO 2 nanoparticles after doping with N [4]. Protein kinase inhibition is the preliminary anticancer assay and previous reports have documented very good anticancer activity of TiO 2 nanoparticles [41,42,43,44].…”

Section: Resultsmentioning

confidence: 99%

Appraisal of Comparative Therapeutic Potential of Undoped and Nitrogen-Doped Titanium Dioxide Nanoparticles

Ahmad

Yang

et al. 2019

Molecules

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Nitrogen-doped and undoped titanium dioxide nanoparticles were successfully fabricated by simple chemical method and characterized using x-ray diffraction (XRD), scanning electron microscopy (SEM), energy dispersive x-ray (EDX), and transmission electron microscopy (TEM) techniques. The reduction in crystalline size of TiO2 nanoparticles (from 20–25 nm to 10–15 nm) was observed by TEM after doping with N. Antibacterial, antifungal, antioxidant, antidiabetic, protein kinase inhibition and cytotoxic properties were assessed in vitro to compare the therapeutic potential of both kinds of TiO2 nanoparticles. All biological activities depicted significant enhancement as a result of addition of N as doping agent to TiO2 nanoparticles. Klebsiella pneumoniae has been illuminated to be the most susceptible bacterial strain out of various Gram-positive and Gram-negative isolates of bacteria used in this study. Good fungicidal activity has been revealed against Aspergillus flavus. 38.2% of antidiabetic activity and 80% of cytotoxicity has been elucidated by N-doped TiO2 nanoparticles towards alpha-amylase enzyme and Artemia salina (brine shrimps), respectively. Moreover, notable protein kinase inhibition against Streptomyces and antioxidant effect including reducing power and % inhibition of DPPH has been demonstrated. This investigation unveils the more effective nature of N-doped TiO2 nanoparticles in comparison to undoped TiO2 nanoparticles indicated by various biological tests. Hence, N-doped TiO2 nanoparticles have more potential to be employed in biomedicine for the cure of numerous infections.

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Section: Resultsmentioning

confidence: 99%

Appraisal of Comparative Therapeutic Potential of Undoped and Nitrogen-Doped Titanium Dioxide Nanoparticles

Ahmad

Yang

et al. 2019

Molecules

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“…We did endotoxin content analyses of the TiO 2 NPs using Limulus amebocyte assay as described in Sree Latha et al . [ 8 ] The amount of endotoxin detected in 1 μg of the TNP, TFP, and TNT injected into mice was 0.5, 0.4, and 0.35 pg, respectively, which did not stimulate the production of any cytokines in the mouse ligated ileal loops.…”

Section: Methodsmentioning

confidence: 99%

“…However, the properties of TiO 2 nanomaterials to the immune system are not well known. Based on our published [ 8 ] and unpublished data, we hypothesized that “TiO 2 has immunomodulatory properties and may lower the immune responses and would be useful for the treatment of graft versus host diseases and autoimmune disorders.” In the present work, we investigated the effect of TiO 2 nanostructural materials such as synthesized H 2 Ti 3 O 7 titanium dioxide nanotubes (TNT), commercial TNP, and TiO 2 fine particles (TFP) on proliferation of mouse splenocytes. Spleen, is the body's largest blood filter, in addition, most important immune reactions like antifungal/antibacterial occur in it.…”

Section: Introductionmentioning

confidence: 99%

Immunomodulatory properties of titanium dioxide nanostructural materials

et al. 2017

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OBJECTIVES:Although titanium dioxide (TiO2) nanostructural materials have been widely used in Biology and Medicine, very little is known about immunomodulation mechanism of these materials. Objectives of this study are to investigate in vitro immunomodulatory effects of TiO2. Immunosuppressant may lower immune responses and are helpful for the treatment of graft versus host diseases and autoimmune disorders.MATERIALS AND METHODS:In this study, we used H2Ti3O7 titanium dioxide nanotubes (TNT) nanotubes along with commercial TiO2 nanoparticles (TNP) and TiO2 fine particles (TFP). We investigated the in vitro immunomodulatory effects of TNP, TNT, and TFP using mixed lymphocyte reaction (MLR). Suppression was studied by 3-(4, 5-dimethylthiazol-2yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay. Cytokine profile was measured by enzyme-linked immunosorbent assay (ELISA).RESULTS AND CONCLUSIONS:The results from this study illustrated that the TiO2 nanostructural materials strongly suppressed splenocytes proliferation in MLR. For TNP and TNT, at 50 μg/ml suppression of 20%–25% and 30%–35%, respectively, and for TFP at 100 μg/ml suppression was 25%–30% was observed. Suppression of splenocytes proliferation in the presence of TNP, TNT, and TFP demonstrated that these nanostructural materials probably block T-cell-mediated responses in vitro. Our ELISA results confirmed that significantly lower levels of Th1 type cytokines (interleukin-2, interferon-γ) in the 48 h MLR culture supernatants. Our data suggest that TiO2 nanostructural materials suppress splenocytes proliferation by suppressing Th1 cytokines.

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“…Titanate nanotubes (TNTs) are recently explored nanomaterials with special physicochemical properties and versatile applicability, from engineering [1,2,3,4,5] to therapeutic use [6,7,8,9,10].…”

Section: Introductionmentioning

confidence: 99%

Investigation of the Compressibility and Compactibility of Titanate Nanotube-API Composites

et al. 2018

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The present work aims to reveal the pharma-industrial benefits of the use of hydrothermally synthesised titanate nanotube (TNT) carriers in the manufacturing of nano-sized active pharmaceutical ingredients (APIs). Based on this purpose, the compressibility and compactibility of various APIs (diltiazem hydrochloride, diclofenac sodium, atenolol and hydrochlorothiazide) and their 1:1 composites formed with TNTs were investigated in a comparative study, using a Lloyd 6000R uniaxial press instrumented with a force gauge and a linear variable differential transformer extensometer. The tablet compression was performed without the use of any excipients, thus providing the precise energetic characterisation of the materials’ behaviour under pressure. In addition to the powder functionality test, the post-compressional properties of the tablets were also determined and evaluated. The results of the energetic analysis demonstrated that the use of TNTs as drug carriers is beneficial in every step of the tabletting process: besides providing better flowability and more favourable particle rearrangement, it highly decreases the elastic recovery of the APIs and results in ideal plastic deformation. Moreover, the post-compressional properties of the TNT–API composites were found to be exceptional (e.g., great tablet hardness and tensile strength), affirming the above results and proving the potential in the use of TNT carriers for drug manufacturing.

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In vitro and in vivo evaluation of anti-cancer activity: Shape-dependent properties of TiO 2 nanostructures

Cited by 33 publications

References 43 publications

Appraisal of Comparative Therapeutic Potential of Undoped and Nitrogen-Doped Titanium Dioxide Nanoparticles

Appraisal of Comparative Therapeutic Potential of Undoped and Nitrogen-Doped Titanium Dioxide Nanoparticles

Immunomodulatory properties of titanium dioxide nanostructural materials

Investigation of the Compressibility and Compactibility of Titanate Nanotube-API Composites

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