In vitro and in vivo Studies of Equine �Coital� Exanthema

Bryans, J T; Allen, George P.

doi:10.1159/000393546

Cited by 24 publications

(23 citation statements)

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“…Cell culture and virus: Equine kidney (EK) and testis (ET) cells were cultured in 96-well, flat-bottomed microplates for the virological examination, because EHV3 is highly host specific and grows only in cells of equine origin [3,18,20]. The culture medium consisted of Eagle's minimum essential medium supplemented with 10% heat-inactivated fetal calf serum (Gibco BRL, U.S.A.), 0.3% tryptose phosphate broth, 0.03% L-glutamin and 0.15% sodium bicarbonate.…”

Section: Physicalmentioning

confidence: 99%

“…The culture medium consisted of Eagle's minimum essential medium supplemented with 10% heat-inactivated fetal calf serum (Gibco BRL, U.S.A.), 0.3% tryptose phosphate broth, 0.03% L-glutamin and 0.15% sodium bicarbonate. The 1118 strain [3] of EHV3 was propagated in EK cells and used as the reference one in this study. This virus was originally isolated by Dr. J. T. Bryans (Kentucky University) and maintained in National Institute of Animal Health (Tsukuba, Japan).…”

Section: Physicalmentioning

confidence: 99%

“…There are no systemic symptoms followed by complete healing within 14 days later when uncomplicated [19,20]. EHV3 is primarily transmitted through coitus [1,13,[18][19][20], although there are also evidences supporting the possibility of non-coital spreading through infected fomites and contacts except for coitus [3,4,11,13,15,19,20].…”

mentioning

confidence: 99%

“…EHV3 has been isolated in many countries such as the United States [3,4], Australia [6,17,18], Canada [8], Denmark [2], Norway [11] and England [7]. In Japan, there are no reports on the isolation of EHV3 from affected horses as far as we know, although the presence of horses with clinical symptoms similar to those of ECE had been occasionally noticed in limited regions.…”

mentioning

confidence: 99%

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Occurrence of Equine Coital Exanthema in Pastured Draft Horses and Isolation of Equine Herpesvirus 3 from Progenital Lesions

Seki

Seimiya

Yaegashi

et al. 2004

The Journal of Veterinary Medical Science

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ABSTRACT. During the period from 2001 to the following year, progenital diseases had been epidemic among the draft stallions and mares pastured together in Iwate Prefecture, the northeastern district of Japan. A stallion and 8 of 31 mares were affected in 2001, and 1 of 2 stallions and 10 of 36 mares in 2002. The clinical symptoms consisted of the formation of papules, pustules, ulcers and scabs on the progenital skin and mucosa in stallions and mares. In 2002, Equine herpesvirus 3 (EHV3) was isolated from 2 mares and the glyco protein G gene of the virus detected from a stallion and 4 mares by polymerase chain reaction. Serum neutralizing tests showed that 12 of 38 horses, 10 clinically and 2 subclinically affected, changed to be positive for the EHV3 antibody. The results suggest that the horses were affected with equine coital exanthema (ECE) through coitus. Five mares with the antibody at the pre-pastured period may have been the possible origins of EHV3 infection in 2002, although the exact origin in 2001 remains unknown. The artificial insemination was performed for the prevention of ECE spreading through coitus on the pasture in 2003. There was no epidemic of the disease in 31 mares, although 3 mares with the antibody at the pre-pastured period showed the significant increase in the titers during the pastured period.

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Section: Physicalmentioning

confidence: 99%

Section: Physicalmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Occurrence of Equine Coital Exanthema in Pastured Draft Horses and Isolation of Equine Herpesvirus 3 from Progenital Lesions

Seki

Seimiya

Yaegashi

et al. 2004

The Journal of Veterinary Medical Science

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“…르기까지 다양한 임상증상을 나타내는 급성 생식기 감염증이 며 말 생산성에 직접적인 영향을 미친다 [4,13]. 말구진은 19세기와 20세기 초반에 유럽에서 발생하고 있었 고 [23] 1968년 캐나다 [8]와 미국에서 처음으로 EHV-3를 분리 하였다 [2,5]. 그 후 전 세계에서 발생하고 있으며 [17,23], 일본 에서도 발생되었으나 [20] 아직 국내의 보고는 없었다.…”

Section: Comparison Of the Nucleotide Sequences Of Gg Gene From Ehv-3unclassified

Characteristics on Equine Herpesvirus Type 3 from Korea

Yang¹,

Lim

2011

Journal of Life Science

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Equine coital exanthema caused by equine herpesvirus type 3 (EHV-3) is a venereal disease which seriously drops horse reproduction rates. Here, we isolated EHV-3 from infected horses and investigated their biological characteristics. Initial cytopathic effects such as rounding of cells were detected 48 hours post infection of the virus into RK-13 cells. The infected cells were going to detach from the surface of culture flasks 72 hours post infection. The type of isolated viruses from swabbed samples was EHV-3 by PCR analysis. Glycoprotein G (gG) of isolated EHV-3 has a 99.25 percent similarity rate to that of EHV-3 334/74 control strain. The isolated EHV-3 was named Georo strain. Georo strain consisted of four major proteins including 145 kD, 60 kD, 45 kD and 40 kD, as shown by SDS-PAGE analysis. We hope the newly isolated Georo strain of EHV-3 can be used for studying various aspects of Korean equine coital exanthema.

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