In vitro and in vivo studies on biocompatibility of carbon fibres

Rajzer, I.; Menaszek, E.; Bačáková, Lucie; Rom, Monika; Błażewicz, M.

doi:10.1007/s10856-010-4108-3

Cited by 59 publications

(41 citation statements)

References 36 publications

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“…42 These findings are also consistent with literature regarding cell proliferation on synthetic fibers where cell proliferation was greatest in regions of cell aggregation and spreading. 41,43,44 The carbon fiber used in this study had a high degree of basal planes oriented along the fiber axis. The basal planes are formed during the carbonization step of carbon fiber processing.…”

Section: Discussionmentioning

confidence: 99%

Hybrid Carbon-Based Scaffolds for Applications in Soft Tissue Reconstruction

Czarnecki

Lafdi

Joseph

et al. 2012

Tissue Engineering Part A

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Section: Discussionmentioning

confidence: 99%

Hybrid Carbon-Based Scaffolds for Applications in Soft Tissue Reconstruction

Czarnecki

Lafdi

Joseph

et al. 2012

Tissue Engineering Part A

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“…Ca, P, and O are derived from HA. Na may be due to the remained Na 2 HPO 4 or the partial substitution of Ca 21 ions in HA crystal lattice by Na 1 ions. C is derived from chitosan and carbon fibers, and N is due to chitosan.…”

Section: Morphology Of Hccpsmentioning

confidence: 99%

“…Under the hydrothermal conditions, the chitosan/calcium phosphate precursors on PCFFs are converted to the elongated HA nanoplates within the chitosan matrix. Interestingly, chitosan remains on the surfaces of HCCPs [ Figure 4 ) groups, and the absorption peaks at 563 and 604 cm 21 are ascribed to the bending vibration (v 4 ) of the phosphate (PO 4 32 ) groups. 33 The characteristic band of B-type CO 3 22 substitution is observed at 1420 cm 21 (v 3 ).…”

Section: -32mentioning

confidence: 99%

“…20 Besides the good mechanical properties, carbon fibers do not cause rejection and have no toxic effects on cells. 21 However, the biological inertness of carbon fibers hinders the extensive application in clinic, whereas HA has been confirmed to have excellent bioactivity. Therefore, HA/carbon fiber composite is a very promising biomaterial for bone tissue engineering, which combines the advantages of HA and carbon fibers.…”

Section: Introductionmentioning

confidence: 99%

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Hydrothermal fabrication of hydroxyapatite/chitosan/carbon porous scaffolds for bone tissue engineering

et al. 2014

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Porous carbon fiber felts (PCFFs) have great applications in orthopedic surgery because of the strong mechanical strength, low density, high stability, and porous structure, but they are biologically inert. To improve their biological properties, we developed, for the first time, the hydroxyapatite (HA)/chitosan/carbon porous scaffolds (HCCPs). HA/chitosan nanohybrid coatings have been fabricated on PCFFs according to the following stages: (i) deposition of chitosan/calcium phosphate precursors on PCFFs; and (ii) hydrothermal transformation of the calcium phosphate precursors in chitosan matrix into HA nanocrystals. The scanning electron microscopy images indicate that PCFFs are uniformly covered with elongated HA nanoplates and chitosan, and the macropores in PCFFs still remain. Interestingly, the calcium-deficient HA crystals exist as plate-like shapes with thickness of 10-18 nm, width of 30-40 nm, and length of 80-120 nm, which are similar to the biological apatite. The HA in HCCPs is similar to the mineral of natural bone in chemical composition, crystallinity, and morphology. As compared with PCFFs, HCCPs exhibit higher in vitro bioactivity and biocompatibility because of the presence of the HA/chitosan nanohybrid coatings. HCCPs not only promote the formation of bone-like apatite in simulated body fluid, but also improve the adhesion, spreading, and proliferation of human bone marrow stromal cells. Hence, HCCPs have great potentials as scaffold materials for bone tissue engineering and implantation.

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“…A common method for the fabrication of 3D nanofiber scaffolds is electrospinning, producing meshes in the regime of extracellular matrix fibers [8]. For example, by spinning polyacrylonitrile (PAN) with a subsequent carbonization, highly conductive nanofiber scaffolds with biocompatible properties were generated [9]. Disadvantage of electro-spun scaffolds is the small mesh size that impairs cell infiltration of the scaffold.…”

mentioning

confidence: 99%

Flexible tissue-like electrode as a seamless tissue-electronic interface

et al. 2017

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Abstract:Current implantable electrodes facilitate only a low cellular infiltration impairing the long-term integration into the host's tissue. To accomplish a seamless electronic-tissue interface, conductive three-dimensional (3D) scaffolds were generated by carbonization of electro-spun fiber meshes. When introducing NaCl particles as porogens, tailored tissue-like electrodes were generated. Characterization of the porous 3D fiber electrodes demonstrated improved material and electrical characteristics compared to standard carbon fiber meshes or flat gold surfaces. The feasibility of the porous 3D electrodes was assessed by cell culture experiments, confirming the migration of cells into the electrode and the formation of contracting cardiomyocyte clusters. Finally, a complex cardiac co-culture system proved the integration of the tissue into the 3D electrode in long-term culture of 7 weeks. These results strengthen the development of tissue-like 3D scaffolds as alternative to two-dimensional (2D) electrodes. The main challenge in the development of implantable electrodes is to improve the currently limited long-term tissue integration that induces adverse effects such as the encapsulation of the implant [1], [2]. This drawback is caused by differences in elasticity between the host tissue and the electrode, an insufficient electrode flexibility to follow tissue deformation as well as surface properties impairing the infiltration of the electrode with cells [3], [4]. Therefore, concepts for the generation of flexible electrodes were developed, consisting of microscopic conducting paths embedded in flexible silicone or SU-8 photoresist [5], [6]. However, the infiltration of cells in the electrodes is still limited, although Bryers et al. [7] showed that the presence of interconnected pores, which facilitate cell infiltration in an implant, significantly reduces the foreign body reaction. Thus, an improved approach may be a seamless 3D tissue-electronic interface composed of conductive nanofibers that are embedded in the tissue. A common method for the fabrication of 3D nanofiber scaffolds is electrospinning, producing meshes in the regime of extracellular matrix fibers [8]. For example, by spinning polyacrylonitrile (PAN) with a subsequent carbonization, highly conductive nanofiber scaffolds with biocompatible properties were generated [9]. Disadvantage of electro-spun scaffolds is the small mesh size that impairs cell infiltration of the scaffold. The generation of electro-spun porous scaffolds by introducing porogens like sugar or NaCl into a non-conductive nanofiber scaffold during the spinning process has already been reported by different groups [10], [11]. Although the highly flexible polymer fibers demonstrated the infiltration of high cell numbers, the obtained pores were susceptible to collapse. Moreover, these porous polymer scaffolds are not applicable as electrodes due to their insulating properties. In our study, we fabricated conductive porous fiber scaffolds. Therefore, we combined conductive nan...

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In vitro and in vivo studies on biocompatibility of carbon fibres

Cited by 59 publications

References 36 publications

Hybrid Carbon-Based Scaffolds for Applications in Soft Tissue Reconstruction

Hybrid Carbon-Based Scaffolds for Applications in Soft Tissue Reconstruction

Hydrothermal fabrication of hydroxyapatite/chitosan/carbon porous scaffolds for bone tissue engineering

Flexible tissue-like electrode as a seamless tissue-electronic interface

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