In vitro and in vivo transfection efficiency of a novel ultradeformable cationic liposome

Kim, Adele; Lee, Eun Hye; Choi, Sangdun; Kim, Chong‐Kook

doi:10.1016/s0142-9612(03)00534-9

Cited by 103 publications

(64 citation statements)

References 28 publications

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“…In some of these articles, either the activity of reporter genes (measured in light emission units) [43,52], or immune response against reporter genes, such as β-galactosidase [20,53], or messenger RNA expression [54], was presented.…”

Section: Discussionmentioning

confidence: 99%

In vivo cutaneous interferon‐γ gene delivery using novel dicationic (gemini) surfactant–plasmid complexes

Badea

Verrall

Baca-Estrada

et al. 2005

The Journal of Gene Medicine

113

129

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The feasibility of topical delivery of pGTmCMV.IFN-GFP plasmid in mice using gemini cationic surfactant based delivery systems was demonstrated. IFNgamma expression after treatment with gemini-DNA formulations in the skin was 3-5-fold higher compared to the treatment with naked DNA (p<0.05), and 4-6-fold higher than the Dc-chol-DNA complex, indicating a significant advance in topical DNA delivery across intact skin in vivo.

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Section: Discussionmentioning

confidence: 99%

In vivo cutaneous interferon‐γ gene delivery using novel dicationic (gemini) surfactant–plasmid complexes

Badea

Verrall

Baca-Estrada

et al. 2005

The Journal of Gene Medicine

113

129

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“…In vivo transfection methods using recombinant viral vectors or nonviral drug delivery system (DDS) carriers, such as cationic polymers and liposomes, have been investigated widely [1][2][3][4] ; however, the methods that deliver naked nucleic acids using physical stimuli are considered the most straightforward because of low toxicities associated with the transfection agents, convenient preparation, ease of handling, and so on.…”

mentioning

confidence: 99%

Liver Suction-Mediated Transfection in Mice Using a Pressure-Controlled Computer System

Shimizu

Zhang

Kawakami

et al. 2014

Biological & Pharmaceutical Bulletin

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We previously developed an in vivo tissue suction-mediated transfection method (denoted as the tissue suction method) for naked nucleic acids, such as plasmid DNA (pDNA) and small interfering RNA (siRNA), in mice. However, it remains unclear whether the suction pressure conditions affect the results of this method. Therefore, in the present study, we assembled a computer system to control the suction pressure and investigate the effects of the suction pressure conditions on the efficiency of the liver suction transfection of naked pDNA that encodes luciferase in mice. Using the developed system, we examined the effects of the minimum magnitude of the suction pressure, suction pressure waveform, and suction times of the luciferase expression level in mice livers. We determined that the liver suction method at 5 kPa was not only effective but also caused the lowest hepatic toxicity in mice. Additionally, the results indicated that the suction pressure waveform affects the luciferase expression levels, and a single period of suction on the targeted portion of the liver is sufficient for transfection. Thus, the developed system is useful for performing the tissue suction method with high accuracy and safety.

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“…Epicutaneous drug administration of these highly deformable carriers was also observed to prolong the biological response time markedly and to increase the reproducibility of the biological drug action [113]. In another study done by Cevc and Blume, either hydrocortisone or dexamethasone formulations in a suspension of very deformable vesicles (transfersomes) have significantly lowered the therapeutically relevant concentration range to 0.1 wt% and may be lower than 0.01 wt%, respectively [114].…”

Section: Transfersomes For Transdermal Delivery Of Drugsmentioning

confidence: 94%

Hydrogels and Their Combination with Liposomes, Niosomes, or Transfersomes for Dermal and Transdermal Drug Delivery

Ibrahim¹,

Nair²,

Al‐Dhubiab³

et al. 2017

Liposomes

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Polymeric networks that retain and absorb substantial amount of water or biological fluids and resemble as a biological tissue are defined as hydrogels. On the other hand, liposomes, transfersomes and niosomes are lipid carriers, which represent one of the major research and development focus areas of the pharmaceutical industry. They have great potential as lipid vehicles that are able to enhance permeation of drugs across the intact skin and can act as local depot for the drug to sustain and control its delivery. Lipid carrier and hydrogel combinations offer transdermal drug delivery of great potential to enhance systemic effects of both hydrophilic and lipophilic drugs. Also, lipid carriers can target drugs to skin appendages and improve transdermal delivery. Lipid carrier proform systems in the form of gelly liquid crystals can also be used transdermally for better drug absorption enhancement. This review highlights the potential of hydrogels and emulgels with or without lipid nanocarriers for dermal and transdermal application.

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In vitro and in vivo transfection efficiency of a novel ultradeformable cationic liposome

Cited by 103 publications

References 28 publications

In vivo cutaneous interferon‐γ gene delivery using novel dicationic (gemini) surfactant–plasmid complexes

In vivo cutaneous interferon‐γ gene delivery using novel dicationic (gemini) surfactant–plasmid complexes

Liver Suction-Mediated Transfection in Mice Using a Pressure-Controlled Computer System

Hydrogels and Their Combination with Liposomes, Niosomes, or Transfersomes for Dermal and Transdermal Drug Delivery

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