In vitro and in vivo Antidiabetic Evaluation of Synthesised Novel New Chromane and its Derivatives

Abstract

Background: To evaluate biological activity of synthesised new chromane and its analogues. Materials and Methods: New chromane {3,5,7-trihydroxy 2-(4-hydroxy benzyl) chroman-4-one} isolated from dried leaves of Dillenia indica Linn., family Dilleniaceae is structurally relating with various reported chroman-4-one derivatives displaying remarkable in vivo antidiabetic activity. But the literature reveals that 0.8 -1.0% yield of pure new chromane was obtained in isolation. Following reported literature data of synthesis and in silico study; Synthesized new chromane and its derivatives (S23-S32) were investigated for in vitro (α-amylase and α-glucosidase) as well as in vivo antidiabetic evaluation respectively. Result and Conclusion: in vitro hypoglycaemic study also displayed the significant antidiabetic potential of new chromane and its O-alkyl substituents (especially S23) while other synthesized compounds (S27-S32) reported for moderate to mild effects w.r.t. reference drug (acarbose). Moreover, synthetic new chromane and O-alkyl substituent (S23) exhibited maximum antidiabetic activity also in terms of lowering glucose concentration while others (S27-S32) showed mild anti-diabetic effect in comparison to reference drug (metformin).