In Vitro and In Vivo Activities of Drugs against Mycobacterium marinum in Yellowtail Seriola quinqueradiata

Abstract

ABSTRACT-Mycobacterium marinum (NJB 0419, MY 0401) isolated from yellowtail Seriola quinqueradiata showed drug susceptibility to rifampin, streptomycin (SM), ethambutol, kanamycin, sulfamethoxazole, amikacin, ciprofloxacin and ofloxacin by the broth dilution method. Furthermore, SM, kanamycin and sulfamonomethoxine activities were tested using the proportion method, and M. marinum was most susceptible to SM. Therefore, SM was used for the treatment of yellowtail (mean body weight 236 g) intramuscularly injected with NJB 0419 at a dose of 5.6 ¥ 10 4 CFU/fish. The diet medicated with SM was orally administered at doses of 25 and 50 mg/kg body weight/day for 7 consecutive days after 4 h or 10 days following injection. Fish receiving 50 mg SM had lower mortality than the controls when medication started 4 h after injection. Therefore, SM may be an effective drug to control M. marinum infection. The timing for initiating oral medication with SM requires further study.

Anti‐tuberculosis effect of isoniazid scales accurately from zebrafish to humans

“…\Mycobacterium marinum and M. tuberculosis show different sensitivities to isoniazid. Isoniazid MIC against M. marinum ranges between 1.6 and 32 mgÁL −1 (Aubry et al, 2000;Boot et al, 2016;Boot et al, 2017;Weerakhun, Hatai, Murase, & Hirae, 2008), in concordance with the value obtained in our analysis. The MIC against M. tuberculosis is lower and ranges from 0.016 to 0.2 mgÁL −1 (Budha, Lee, Hurdle, Lee, & Meibohm, 2009;Gumbo et al, 2007;Hemanth Kumar et al, 2016;Jayaram et al, 2004;Schön et al, 2009).…”