Infection with &lt;i&gt;Mycobacterium pseudoshottsii&lt;/i&gt; in Cultured Yellowtail &lt;i&gt;Seriola quinqueradiata&lt;/i&gt; in Owase Bay, Japan

Matsumoto, Megumi; Machida, Yuichiro; Kanemaru, Motohisa; Yamamoto, Masaaki; Sano, Motohiko; Kato, Go

doi:10.3147/jsfp.57.35

Cited by 3 publications

(3 citation statements)

References 16 publications

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“…Whilst rifampicin and rifabutin have been shown to be the most active drugs against M. marinum variant infections [ 80 ] they are often treated with tritherapies including rifampicin, clarithromycin and ethambutol [ 81 , 82 ] thus slightly differing from M. ulcerans variant therapy. Amikacin, ciprofloxacin, kanamycin and lincomycin inhibited the growth of M. pseudoshottsii variant isolates [ 83 ]. In addition to being resistant to isoniazid, ethambutol and ethionamide, the M. liflandii variant also exhibits resistance to rifampicin (rifampin) and clarithromycin [ 84 , 85 ], suggesting that the combination of these later two antibiotics may not be appropriate to treat BU cases caused by M. liflandii variants.…”

Section: Implication For the Diagnosis And Treatment Of Bumentioning

confidence: 99%

What about Current Diversity of Mycolactone-Producing Mycobacteria? Implication for the Diagnosis and Treatment of Buruli Ulcer

Combe,

Cherif,

Blaizot

et al. 2023

IJMS

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The identification of an emerging pathogen in humans can remain difficult by conventional methods such as enrichment culture assays that remain highly selective, require appropriate medium and cannot avoid misidentifications, or serological tests that use surrogate antigens and are often hampered by the level of detectable antibodies. Although not originally designed for this purpose, the implementation of polymerase-chain-reaction (PCR) has resulted in an increasing number of diagnostic tests for many diseases. However, the design of specific molecular assays relies on the availability and reliability of published genetic sequences for the target pathogens as well as enough knowledge on the genetic diversity of species and/or variants giving rise to the same disease symptoms. Usually designed for clinical isolates, molecular tests are often not suitable for environmental samples in which the target DNA is mixed with a mixture of environmental DNA. A key challenge of such molecular assays is thus to ensure high specificity of the target genetic markers when focusing on clinical and environmental samples in order to follow the dynamics of disease transmission and emergence in humans. Here we focus on the Buruli ulcer (BU), a human necrotizing skin disease mainly affecting tropical and subtropical areas, commonly admitted to be caused by Mycobacterium ulcerans worldwide although other mycolactone-producing mycobacteria and even mycobacterium species were found associated with BU or BU-like cases. By revisiting the literature, we show that many studies have used non-specific molecular markers (IS2404, IS2606, KR-B) to identify M. ulcerans from clinical and environmental samples and propose that all mycolactone-producing mycobacteria should be definitively considered as variants from the same group rather than different species. Importantly, we provide evidence that the diversity of mycolactone-producing mycobacteria variants as well as mycobacterium species potentially involved in BU or BU-like skin ulcerations might have been underestimated. We also suggest that the specific variants/species involved in each BU or BU-like case should be carefully identified during the diagnosis phase, either via the key to genetic identification proposed here or by broader metabarcoding approaches, in order to guide the medical community in the choice for the most appropriate antibiotic therapy.

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Section: Implication For the Diagnosis And Treatment Of Bumentioning

confidence: 99%

What about Current Diversity of Mycolactone-Producing Mycobacteria? Implication for the Diagnosis and Treatment of Buruli Ulcer

Combe,

Cherif,

Blaizot

et al. 2023

IJMS

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“…The nontuberculous mycobacteria (NTM) are a large group of pathogens that are capable of causing chronic and severe disease in a vast number of living organisms ( 1 ). The Mycobacterium ulcerans / Mycobacterium marinum clade (MuMC) is an important group of NTM that shows high genomic similarities among its four members: Mycobacterium ulcerans , which causes a necrotizing disease of the skin and soft tissue and is also known as Buruli ulcer, especially in African countries and Australia ( 2 ); Mycobacterium marinum , which is one of the most important fish pathogens but is also known as a human pathogen ( 3 ); Mycobacterium shottsii , which is a fish-pathogenic mycobacterium that was originally isolated from wild, diseased striped bass ( Morone saxatilis ) in the Chesapeake Bay, USA, with a limited geographical distribution ( 4 ); and Mycobacterium pseudoshottsii , which is a fish pathogen that was also first detected in wild, diseased striped bass in the Chesapeake Bay ( 5 ) but, with time, was also diagnosed in farmed fish species from Japan ( 6 ) and Europe ( 7 ).…”

Section: Observationmentioning

confidence: 99%

Identification of Mycobacterium pseudoshottsii in the Eastern Mediterranean

Davidovich,

Makhon,

Zizelski Valenci

et al. 2023

Microbiol Spectr

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“… Mycobacterium pseudoshottsii , a slow-growing nontuberculous mycobacterium that can cause mycobacteriosis in fish ( 1 ), has been reported in wild and cultured fish ( 2 – 5 ). This pathogen produces a macrolide toxin, mycolactone, and is classified as a mycolactone-producing mycobacterium ( 6 ).…”

Section: Announcementmentioning

confidence: 99%

Complete Genome and Partial Megaplasmid Sequences of Mycobacterium pseudoshottsii Strain NJB1907-Z4, Isolated from an Aquarium-Reared Japanese Sardine (Sardinops melanostictus) in Japan

Komine

Fukano

Yoshida

et al. 2022

Microbiol Resour Announc

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Infection with <i>Mycobacterium pseudoshottsii</i> in Cultured Yellowtail <i>Seriola quinqueradiata</i> in Owase Bay, Japan

Cited by 3 publications

References 16 publications

What about Current Diversity of Mycolactone-Producing Mycobacteria? Implication for the Diagnosis and Treatment of Buruli Ulcer

What about Current Diversity of Mycolactone-Producing Mycobacteria? Implication for the Diagnosis and Treatment of Buruli Ulcer

Identification of Mycobacterium pseudoshottsii in the Eastern Mediterranean

Complete Genome and Partial Megaplasmid Sequences of Mycobacterium pseudoshottsii Strain NJB1907-Z4, Isolated from an Aquarium-Reared Japanese Sardine (Sardinops melanostictus) in Japan

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