In vitro and in vivo antioxidant activities of the aqueous extract of Celosia argentea leaves

Abstract

Objective:The aqueous extract of Celosia argentea var. cristata L. leaves at 100, 200, and 400 mg/kg body weight (b.w.) was investigated against cadmium (Cd)-induced oxidative stress in Wistar rats. The in vitro antioxidant of the extract was evaluated using ammonium thiocyanate, reducing power, and membrane stabilizing models.Materials and Methods:For the in vivo study, 30 male rats (Rattus norvegicus) weighing 138.02 ± 7.02 g were completely randomized into 6 groups (A–F) of 5 animals each. Animals in groups A and B received 0.5 ml of distilled water and the same volume containing 8 mg/kg b.w. of Cd, respectively, for 7 days orally. Animals in groups C, D, E, and F were treated like those in group B except that they received 100 mg/kg b.w. of ascorbic acid, and 100, 200, and 400 mg/kg b.w. of the extract, respectively, in addition to Cd.Results:Phytochemical screening revealed the presence of alkaloids (0.61%), saponins (2.93%), cardiac glycosides (0.21%), cardenolides (0.20%), phenolics (3.26%), and flavonoids (2.38%). A total of 10 mg/ml of the extract inhibited linoleic acid oxidation by 67.57%. The highest reducing power was 100 mg/ml as against 10 mg/ml for ascorbic acid. In addition, 2 mg/ml of the extract produced a membrane stabilizing activity of 63.49% as against 77.46% for indomethacin. Compared with the distilled water control group, the administration of Cd alone significantly (P < 0.05) decreased the alkaline phosphatase activity of the rat liver and brain. This decrease was accompanied by a corresponding increase in the serum enzyme. The simultaneous administration of the extract and Cd produced an enzyme activity that compared favorably (P > 0.05) with the animals that received Cd and ascorbic acid. In addition, the reduction in the superoxide dismutase and catalase activity of the liver and brain of the animals, serum uric acid, albumin and bilirubin, and also the increase in the serum malondialdehyde content in animals treated with Cd alone was attenuated by the extract; the values compared well (P > 0.05) with those simultaneously administered with Cd and ascorbic acid.Conclusion:Overall, the results indicated that the aqueous extract of C. argentea leaves attenuated Cd-induced oxidative stress in the animals, with the best result at 400 mg/kg b.w. The antioxidant activity of the extract may be attributed to the phenolic and flavonoid components of the extract. The induction of antioxidant enzymes and scavenging of free radicals may account for the mechanism of action of the extract as an antioxidant.

Antinociceptive effect of methanol extract of Celosia cristata Linn. in mice

“…30 min before the experiments. The doses of MECC were selected from trial and previously reported effective doses of C. cristata [ 29 ]. To evaluate the involvement of opioid-mediated antinociceptive activity, naloxone was administered (i.p.)…”

Kolaviron and L-Ascorbic Acid Attenuate Chlorambucil-Induced Testicular Oxidative Stress in Rats

“…and L-ascorbic acid (100 mg/kg b.w.). The dose of chlorambucil administered to the animals was based on the recommended dose in the treatment of Hodgkin's lymphoma (0.1-0.2 mg/kg/day) [ 33 ], while doses of ascorbic acid and kolaviron were selected based on the results obtained from previous studies in experimental animals [ 34 , 35 ]. Each of the drug doses was delivered orally in 1 mL solution, once daily using an oral cannula.…”

Computational and experimental validation of antioxidant properties of synthesized bioactive ferulic acid derivatives

“…The assay was performed as described by Malomo et al [34] The assay mixture consisted of PBS, indomethacin (reference drug) or different concentrations of FA or its derivatives, and 2% erythrocyte suspension in isosaline. The control was prepared without the reference drug (or FA/derivatives) whereas the drug control and the FA control lacked erythrocyte suspension.…”