In vitro and in vivo investigation of a zonal microstructured scaffold for osteochondral defect repair

Steele, Joseph A. M.; Moore, Axel C.; St-Pierre, Jean-Philippe; McCullen, Seth D.; Gormley, Adam J.; Horgan, Conor C.; Black, Cameron; Meinert, Christoph; Saifzadeh, Siamak; Steck, Roland; Ren, Jiongyu; Woodruff, Maria A.; Stevens, Molly M.

doi:10.1016/j.biomaterials.2022.121548

Cited by 32 publications

(28 citation statements)

References 55 publications

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“…PCL fibers were also used in another study to reproduce the orientational features of collagen fibers in each OC region, and thereby generating a scaffold with gradient stiffness mimicking that of OC tissue. This particular design positively impacted its osteointegration [ 134 ]. However, potential drawbacks of the MZSs with gradient structures include poor spatial distribution of differentiated cells and low reproducibility [ 86 ] that can be related to the lack of control on the porous structure.…”

Section: Biomimetic Architectures In Mzsmentioning

confidence: 99%

“…PCL is a biocompatible polymer with a low printing temperature that makes it suitable for various fabrication techniques [ 134 ], especially 3D printing [ 197 ] and electrospinning [ 74 , 94 ]. It has good mechanical properties [ 198 ] that can be tailored by adjusting its molecular weight [ 199 ].…”

Section: Recent Biomaterials Selections For Fabrication Of Mzssmentioning

confidence: 99%

“…It has been found that PCL is efficient in facilitating chondrogenic differentiation, cartilage matrix production, and cartilage-like tissue formation [ 74 , 94 , 97 , 121 , 188 ], but osteogenic differentiation or neobone formation was only achieved in the presence of bioceramics in the bone layer [ 70 , 74 , 94 ]. PCL is nonetheless a versatile material that can be used as a main component in both cartilage and bone layers, as demonstrated by Steele et al [ 134 ]. In this study, four manufacturing methods, electrospinning, porogen leaching, directional freezing, and melt electrowriting (MEW), were considered to fabricate PCL-only scaffolds with gradient stiffness and porosity.…”

Section: Recent Biomaterials Selections For Fabrication Of Mzssmentioning

confidence: 99%

“…DEX has therefore been orally or intravenously administrated to control inflammation and reduce joint damage [ [460] , [461] , [462] , [463] ]. It has also long been used as a supplement of cell culture medium to induce chondrogenic differentiation of cells [ 134 , 464 , 465 ]. It was discovered that localized and sustained release of low-dose DEX from an acellular agarose hydrogel could enhance osteochondral repair through a dual pro-anabolic and anti-catabolic effect to attenuate inflammation and support the functional integrity between the acellular graft material and host tissue in a preclinical canine autograft model [ 466 ].…”

Section: Cell Sources and Growth Factors In Mzssmentioning

confidence: 99%

“…It enhances OC repair through a dual pro-anabolic and anti-catabolic effect to attenuate inflammation. [ 134 , [464] , [465] , [466] , [467] ] KGN CL It acts as a chondrogenic and chondroprotective agent to induce cartilage regeneration, but its clinical application is limited by its hydrophobicity and low water solubility as it forms precipitates in cells, leading to ineffective chondrogenic stimulation. [ 188 , [469] , [470] , [471] , [472] , [473] , [474] , [475] , [476] , [477] , [478] , [479] , [480] , [481] , [482] ] …”

Section: Cell Sources and Growth Factors In Mzssmentioning

confidence: 99%

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Recent development in multizonal scaffolds for osteochondral regeneration

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Section: Biomimetic Architectures In Mzsmentioning

confidence: 99%

Section: Recent Biomaterials Selections For Fabrication Of Mzssmentioning

confidence: 99%

Section: Recent Biomaterials Selections For Fabrication Of Mzssmentioning

confidence: 99%

Section: Cell Sources and Growth Factors In Mzssmentioning

confidence: 99%

Section: Cell Sources and Growth Factors In Mzssmentioning

confidence: 99%

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Recent development in multizonal scaffolds for osteochondral regeneration

Cavelier

Hannon

et al. 2023

Bioactive Materials

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Glass, Ceramic, Polymeric, and Composite Scaffolds with Multiscale Porosity for Bone Tissue Engineering

Jeyachandran

Cerruti

2023

Adv Eng Mater

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Porosity affects performance of scaffolds for bone tissue engineering both in vitro and in vivo. Macropores (i.e., pores with a diameter >100 μm) are essential for cellular infiltration; micropores (i.e., pores with a diameter of 1–10 μm) promote cell adhesion and facilitate nutrient absorption. Scaffolds containing both macropores and micropores exploit the advantages of both pore sizes and have excellent osteogenic properties. Nanopores (i.e., pores with a diameter of 1–50 nm) can be included as well, to improve cell–material interactions by further enhancing the surface area of the scaffold. This article reviews fabrication techniques and properties of scaffolds with multiscale porosity, focusing on glass, ceramic, polymeric, and composite scaffolds. After discussing the structure of bone and how it inspired scaffolds for bone tissue engineering, pore nomenclature is introduced. Then, the techniques used to induce multiscale porosity, the nature of the pores created, and the effects of scaffold porosity on mechanical properties and biological activity of the scaffolds are discussed. The review concludes by providing an outlook for this field, including advancements that are made possible by computational modeling and artificial intelligence.

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Nanoenabled Immunomodulatory Scaffolds for Cartilage Tissue Engineering

Pereira Vasconcelos,

Leite Pereira,

Couto

et al. 2024

Adv Funct Materials

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Articular cartilage regeneration is a challenge in tissue engineering. Although diverse materials have been developed for this purpose, cartilage regeneration remains suboptimal. The integration of nanomaterials into 3D network materials holds great potential in the improvement of key mechanical properties, particularly important for osteochondral replacement scaffolds and even to function as carriers for disease‐modifying drugs or other regulatory signals. In this study, a simple yet effective cell‐free nanoenabled Col‐PLA scaffold specially designed to enhance cartilage regeneration and modulate inflammatory response is proposed, by incorporating poly(lactic‐co‐glycolic acid) (PLGA) ibuprofen nanoparticles (NPs) into a collagen/polylactide (Col‐PLA) matrix. The developed nanoenabled scaffold successfully decreases IL‐1β release and leads to primary human chondrocytes survival, ultimately restoring extracellular matrix (ECM) production under inflammatory conditions. The nanoenabled Col‐PLA scaffolds secretome effectively decreases macrophage invasion in vitro, as well as neutrophil infiltration and inflammatory mediators’, namely the complement component C5/C5a, C‐reactive protein, IL‐1β, MMP9, CCL20, and CXCL1/KC production in vivo in a rodent air‐pouch model. Overall, the established nanoenabled scaffold has the potential to support chondrogenesis as well as modulate inflammatory response, overcoming the limitations of traditional tissue engineering strategies.

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In vitro and in vivo investigation of a zonal microstructured scaffold for osteochondral defect repair

Cited by 32 publications

References 55 publications

Recent development in multizonal scaffolds for osteochondral regeneration

Recent development in multizonal scaffolds for osteochondral regeneration

Glass, Ceramic, Polymeric, and Composite Scaffolds with Multiscale Porosity for Bone Tissue Engineering

Nanoenabled Immunomodulatory Scaffolds for Cartilage Tissue Engineering

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