In vitro and In vivo Anti-inflammatory Activity of the Extracts of Whole Plant Argyreia imbricata (Roth) Sant. & Patel

Abstract

Plants of the genus Argyreia have ethnomedicinal importance, and several pharmacological activities are also reported. In this study, the anti-inflammatory activity of different extracts of Argyreia imbricata was evaluated by in vitro and in vivo methods. In both evaluations, standard, Diclofenac sodium was used for comparative evaluation. In this study, extraction of powdered whole plant material was done with different solvents viz., petroleum ether, chloroform, ethyl acetate and methanol by soxhelation. In vitro anti-inflammatory activity of all the prepared extracts was evaluated by stabilization of human red blood cell (HRBC) membrane in different temperature and tonicity conditions. Among the six different concentrations of four tested extracts, the ethyl acetate and methanol extracts (1000μg/ml) showed significant activity in the in vitro evaluation. They were selected for the in vivo evaluation on the paw oedema induced by carrageenan on Wistar albino rats. Two doses, 200mg.kg-1 and 400mg.kg-1 of the test extracts were subjected to evaluation. Both the tested extracts showed the activity, particularly, the methanol extract in the dose of 400mg.kg-1 showed significant activity. Results of this study strongly supported the anti-inflammatory activity of the tested extracts. Further, studies on toxicity, identification, isolation of the active constituents may give useful results.

In Vitro Anti-Inflammatory Evaluation of Sesbania Procumbens Extracts

“…Methanol extract in 1000µg/ml concentration revealed 67.14±0.72 percentage protection which is comparable with the standard drug. A similar type of outcome were found in previous literatures 7,14 .…”

“…Testing under different temperature conditions (54°C and -10°C) also indicated a concentration-dependent rise in the activity of tested extracts which is similar to the results of previous literatures [13][14][15][16][17][18][19][20][21][22][23][24][25][26][27] Particularly, in the evaluation on cold testing conditions, the methanol extract showed a notable activity again. 1000µg/ml of methanol extract in cold testing conditions showed 79.50 ± 1.20 percentage protection.…”

“…Testing of the anti-inflammatory efficacy of S. procumbens extracts under different tonicity conditionsalso revealed a concentration-dependent rise of activity. Previous literaures [13][14][15][16][17][18][19][20][21][22][23][24][25][26][27] documented similar type of outcome. In this testing approach also the methanol extract showed significant activity particularly in isotonic testing conditions as compared to other extracts tested and the standard drug used for the comparative evaluation (Table 3).…”

“…All the prepared extracts were evaluated for antiinflammatory activity in vitro by human red blood cell (HRBC) membrane stabilization in normal and different temperature and tonicity conditions in reference to the previous literature 13, 14 . AR grade Chemicals of Millipore Sigma were used in all the experiments.…”

“…It may be due to the arrest of inflammatory mediators and lytic enzyme release. Previous literature 13,14,19 . stated that the saponins and flavonoids of plants have a significant effect on lysosomal membrane stabilization in both in vitro and in vivo evaluation.…”