In Vitro and In Vivo Evaluation of Hepatoprotective Activity of Gymnema Sylvestre

Abstract

Leaves of Gymnema sylvestre were extracted by hot and cold maceration. Qualitative phytochemical analysis of the plant extract showed the presence of majority of compounds like alkaloids, carbohydrates, flavonoids, terpienoids, glycosides, proteins, amino acids, phenolic compounds and tannins.. Among the two extracts, the hydro- alcoholic extract prepared by the hot maceration has shown high phenol content 6.51 ± 0.231 mg/g, high total favonolid content 89.51 ± 0.100 mg/g. By DPPH method, total antioxidant capacity, reducing power ability of the hydro-alcoholic extract prepared by hot maceration process was evaluated. The freshly prepared rat hepatocytes were treated with different concentration of hydro-alcoholic extract prepared by the hot maceration process. The antihepatotoxicity produced by the extract at the concentration of 200, 400,600 µg/ml was found effective against the D – galactosamine-induced hepatotoxicity, where as at the concentration of 800 µg/ml was found to be cytotoxic. A significant increase in the levels of ASAT, ALAT,ALP, total bilirubin, direct bilirubin ( P< 0.001) were observed. The cells were treated with the hydro-alcoholic extract different extracts of Gymnema sylvestre showed a significant restoration of the altered biochemical parameters towards the normal (P<0.001) when compared to D- galactosamine treated groups and were found to be dose dependent. A similar result was obtained when D- galactosamine intoxicated hepatocytes were treated with silymarin.

Phytochemical and Pharmacological Properties ofGymnema sylvestre: An Important Medicinal Plant

“…The hepatoprotective effect of hydro-alcoholic extract of G. sylvestre was evaluated by Srividya et al [ 86 ]. The rat hepatocytes (freshly prepared) were subject to treatment with different concentration of hydroalcoholic extract prepared by the hot maceration process.…”

<em>Gymnema Sylvestre</em> Supplementation Restores Normoglycemia and Corrects Dyslipidemia through Transcriptional Modulation of ßeta Cells and Hepatocytes in the Alloxan-Induced Hyperglycemic Rats

“…These bioactive components are oleanane-type triterpenoid saponins and are known as anthraquinones, acidic glycosides, gymnemic acids alkaloids, and their products [18,20]. In-vitro Hepatoprotective activity of GS has also been reported previously [21][22][23]. Previously it has been reported that GS stimulates insulin release in vitro by increasing the membrane permeability of pancreatic beta cells [24][25][26].…”

Simplified Column Fractionation with DPPH Assay Guidance: Isolated a Potent Hepatoprotective Fraction from Cassia auriculata

“…This comprehensive approach assessed the hepatoprotective activity of the C2 Column Fraction in the CCl 4 -induced hepatotoxicity model. [40][41][42]…”