In vitro and in vivo evaluation of pharmacological potential of Begonia barbata Wall

Abstract

Background Begonia barbata Wall is an important medicinal plant in Bangladesh used to treat several human diseases. The intention of the existing work was to determine in vitro and in vivo pharmacological properties of petroleum-ether, dichloromethane, and ethyl acetate soluble fractions of methanol extract of B. barbata whole plant (MEBB). The total phenolic content (TPC) was determined by Folin-Ciocalteu test while antioxidant activity assay was performed by DPPH radical quenching process. Tail immersion and acetic acid-induced writhing methods were utilized to evaluate analgesic effect in albino mice. To evaluate the hypoglycemic potential, glucose tolerance test is used. Castor oil-induced diarrhea method is utilized to figure out the anti-diarrheal action in mice. Thiopental-sodium-induced sleeping time test was exploited for the appraisal of CNS antidepressant action. Results Antioxidant activities of the test materials followed the order: dichloromethane fraction (DCMF) > ethyl acetate fraction (EAF) > petroleum-ether fraction (PEF). The EAF yielded maximum amount of phenolic compounds (12.47 mg of GAE/g of sample). The DMF was most effective in terms of thrombolytic activity while the EAF protected the membrane integrity of erythrocyte by 63.47% inhibition of hemolysis induced by heat. Among all fractions, the PEF (400 mg/kg body weight) demonstrated highest analgesic activity in both tail immersion test (maximum elongations as 262.053%) and acetic acid-induced writhing method (66.02% writhing inhibition). During anti-diarrheal test, the plant samples significantly diminished the frequency of diarrheal episodes in mice. Similarly, the plant samples decreased the sleeping duration induced by thiopental sodium. Conclusion The above outcome exposed that B. barbata possesses significant pharmacological potential, which validates its use in Bangladesh folk medicinal practices.

In vitro and in vivo Pharmacological Studies of Leaves of Staurogyne argentea Wall.

“…Here, acetic acid-induced writhing test was utilized to determine the analgesic effect of S. argentea. Intra-peritoneally administered acetic acid elevates the production of prostaglandin which ultimately leads to inflammation in mice (Haque et al, 2020). The plant extracts noticeably repressed the number of abdominal writhes in mice (Table 3).…”

An insight into novel therapeutic potentials of taxifolin

“…In addition, taxifolin has demonstrated biological activities such as anti-Alzheimer activity ( Sato et al, 2013a ), antimicrobial activity ( Cushnie and Lamb, 2005 ), anticancer activity ( Lee et al, 2007 ), hepatoprotective activity ( Akinmoladun et al, 2018 ), antiangiogenic activity ( Haque et al, 2015 ), cardiovascular activity ( Shu et al, 2019 ) and pulmonary activity ( Jh et al, 2020 ) which make it an effective treatment for a variety of diseases.…”

Seed conservation of Begonia spp. from West Sumatra, Indonesia

“…Begonia is one of the plant genera that have the potential to be developed and utilized. Aside from being an ornamental plant, Begonia can be used as a spice for cooking and vegetables [1], and also used as medicinal raw materials [2,3,4,5]. Begonia research and ex-situ conservation efforts have been carried out intensively for the last ten years at the Cibodas Botanical Gardens (CBG).…”