In vitro and in vivo antitumor effects of doxorubicin loaded with bacterial magnetosomes (DBMs) on H22 cells: The magnetic bio-nanoparticles as drug carriers

Sun, Jianbo; Duan, Jiaqi; Dai, Shunling; Ren, Jun; Zhang, Yandong; Tian, Jie; Liu, Ying

doi:10.1016/j.canlet.2007.08.018

Cited by 105 publications

(62 citation statements)

References 35 publications

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“…Briefly, 54 female Swiss nude mice of 6 week of age (Charles River, Arbresle, France) received by subcutaneous injection two millions of MDA-MB-231 human breast cancer cells, (18), both on the left and right flanks. The injection of the various types of particles has been carried out 14 days after tumor implantation.…”

Section: Biodistribution Studiesmentioning

confidence: 99%

Chains of Magnetosomes Extracted from AMB-1 Magnetotactic Bacteria for Application in Alternative Magnetic Field Cancer Therapy

et al. 2011

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International audienceChains of magnetosomes extracted from magnetotactic bacteria are shown to be highly efficient for alternative magnetic field cancer therapy. The viability of MDA-MB-231 breast cancer cells is relatively unaffected by the presence of less than ∼ 1 mg of chains of magnetosomes. When these cells are exposed to an oscillating magnetic field of frequency 183 kHz and field strengths of 20 to 60 mT, up to 100 % of them are destroyed. We show that it is possible to fully eradicate a tumor xeno-greffed on a mouse by administering a suspension containing ∼ 1 mg of chains of magnetosomes within the tumor and by exposing the mouse to three heat cycles of 20 minutes, during which the tumor temperature is raised to ∼ 43°C. We demonstrate the higher efficiency of the chains of magnetosomes compared with various other materials, i. e. whole inactive magnetotactic bacteria, individual magnetosomes not organized in chains and two different types of chemically synthesized nanoparticles currently tested for alternative magnetic field cancer therapy. The efficiency of the chains of magnetosomes is attributed to three factors, (i), a high magnetosome specific absorption rate (SAR), (ii), a homogenous distribution of the chains of magnetosomes within the tumor yielding uniform heating and (iii), the faculty of the chains of magnetosomes to penetrate within the cancer cells following the application of the alternative magnetic field, which enables intra-cellular heating. Biodistribution studies reveal that chains of magnetosomes administered directly within xeno-greffed breast tumors progressively leave the tumors during the 14 days following their administration and are then eliminated in the feces

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Section: Biodistribution Studiesmentioning

confidence: 99%

Chains of Magnetosomes Extracted from AMB-1 Magnetotactic Bacteria for Application in Alternative Magnetic Field Cancer Therapy

et al. 2011

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“…Previous studies have addressed a variety of applications and properties, including enzyme immobilization (5), gene delivery system (16), cell separation (19), drug carriers (11,12), immunoassays (4,14), protein and multisubunit enzyme complexes (7,20), and use of microorganisms per se for mineral recovery (13). Because of the highly restrictive culture conditions for magnetotactic bacteria, in terms of the dissolved oxygen concentration (dO 2 ) (3, 17), nutrients, etc., the yields of both magnetosomes and their host microorganisms under artificial culture tend to be low (10,18).…”

mentioning

confidence: 99%

Semicontinuous Culture of Magnetospirillum gryphiswaldense MSR-1 Cells in an Autofermentor by Nutrient-Balanced and Isosmotic Feeding Strategies

Zhang

Jiang

et al. 2011

Appl Environ Microbiol

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125

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An improved strategy was developed for the high-density culture of Magnetospirillum gryphiswaldense strain MSR-1 and large-scale magnetosome production in both 7.5-and 42-liter autofermentors. By using a nutrientbalanced feeding strategy and the replacement of carbon and nitrogen sources to reduce accumulation of Na ؉ and Cl ؊ ions, we reduced the factors that tend to inhibit cell growth, particularly the increase of osmotic potential. Semicontinuous culture was thereby achieved in the autofermentor for the first time. When the cells were harvested at 36 and 73 h, magnetosome yields (dry weight) as high as 168.3 and 83.5 mg/liter/day, respectively, were achieved. These values were, respectively, approximately 10 and 5 times higher than the yields achieved in previous studies and represent a significant improvement in magnetosome production efficiency.Biomineralized magnetosomes (chains of magnetite crystals found in prokaryotes) have attracted commercial interest because of their narrow size range, good dispersibility, and biomembrane enclosure. Previous studies have addressed a variety of applications and properties, including enzyme immobilization (5), gene delivery system (16), cell separation (19), drug carriers (11, 12), immunoassays (4, 14), protein and multisubunit enzyme complexes (7,20), and use of microorganisms per se for mineral recovery (13). Because of the highly restrictive culture conditions for magnetotactic bacteria, in terms of the dissolved oxygen concentration (dO 2 ) (3, 17), nutrients, etc., the yields of both magnetosomes and their host microorganisms under artificial culture tend to be low (10, 18). A long-standing research goal of our laboratory is improved large-scale production of cells and magnetosomes.In a previous study using fed-batch culture techniques (10), we achieved maximal cell density (optical density at 565 nm [OD 565 ] of 7.24, cell dry weight of 2.17 g/liter [0.87 g/liter/day], and magnetosome dry weight of 41.7 mg/liter [16.7 mg/liter/ day]). Through further optimization of culture temperature, pH, dO 2 , and nutrients, we achieved an OD 565 value of 12 in a 7.5-liter fermentor after 40 h of culture (unpublished data). In revising our previous feeding strategy, we focused on supplementation of carbon and nitrogen sources but ignored two possible factors that could inhibit cell growth: (i) nutrient limitation arising during fermentation process and (ii) the accumulation of Na ϩ and Cl Ϫ in a fermentor fed with sodium lactate and ammonium chloride. By replacing the carbon and nitrogen sources and using an optimally nutrient-balanced feeding strategy, in a 7.5-liter fermentor after 44 h, we achieved an OD 565 of 30.4, a cell dry weight of 7.59 g/liter (3.8 g/liter/ day), and a magnetosome dry weight of 225.53 mg/liter (112.77 mg/liter/day). In a larger (42-liter) fermentor, after 44 h, we achieved an OD 565 of 42, a cell dry weight of 9.16 g/liter (4.58 g/liter/day), and a magnetosome dry weight of 356.52 mg/liter (178.26 mg/liter/day). The efficiency of magnetosome produ...

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“…Such nanoparticles find diverse applications, especially in biomedical field, such as in magnetic resonance imaging [106], cell tracking [107], drug delivery [108,109], and gene delivery [110].…”

Section: Microbial Cell Factories: Manufacturing Functional Metabolitesmentioning

confidence: 99%

Fabrication of nanocomposites and hybrid materials using microbial biotemplates

Shi

Ullah

et al. 2017

Adv Compos Hybrid Mater

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Microbes are important part of life that vary in sizes and shapes, diverse surface chemistry and biology, and porous nature of their cell walls. Besides their importance in industrial processes such as fermentation, these serve as biotemplates and provide a biomimetic approach for fabrication of multifarious complex constructs with predefined features, ordered composites and hybrid nanomaterials, microdevices, and micro/nanorobots through various strategies. The template or building blocks for such approaches can be bacterial, algal, and fungal cells or virus particles. Here, we have summarized recent advancements in biofabrication based on live microbes. Using engineering approaches and suitable methods, live microbes can be manipulated as functional Bmicro/nanodevices and -robots^to further perform biological functions such as replication, distribution, motility, formation of colonies, and secretion of metabolites at will. Biofabrication based on microbes provides effective methods to control and manipulate microbes as functional live building blocks to create micro/nanodevices and -robots for biomedical and energy applications.

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In vitro and in vivo antitumor effects of doxorubicin loaded with bacterial magnetosomes (DBMs) on H22 cells: The magnetic bio-nanoparticles as drug carriers

Cited by 105 publications

References 35 publications

Chains of Magnetosomes Extracted from AMB-1 Magnetotactic Bacteria for Application in Alternative Magnetic Field Cancer Therapy

Chains of Magnetosomes Extracted from AMB-1 Magnetotactic Bacteria for Application in Alternative Magnetic Field Cancer Therapy

Semicontinuous Culture of Magnetospirillum gryphiswaldense MSR-1 Cells in an Autofermentor by Nutrient-Balanced and Isosmotic Feeding Strategies

Fabrication of nanocomposites and hybrid materials using microbial biotemplates

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