In vitro and in vivo degradation studies for development of a biodegradable patch based on poly(3-hydroxybutyrate)

Evaluation of biocompatibility of random or aligned electrospun polyhydroxybutyrate scaffolds combined with human mesenchymal stem cells

“…Recently, there has been increasing interest in constructing desirable extracellular microenvironments on the surface of biomaterials in order to obtain optimal cell and/or tissue responses. Studies with polyhydroxybutyrate (PHB) membranes and mesenchymal stem cells (MSCs), which have high differentiation capacity, showed that PHB membranes induced MSCs to osteogenic differentiation in vitro and in vivo (Wang et al, 2004;Wollenweber et al, 2006;Rentsch et al, 2010) PHB is the most thoroughly investigated member of the PHA family and has shown good biocompatibility with different cell types, including mouse fibroblast cell lines (Yang et al, 2002), chondrocytes (Saito et al, 1991), osteoblasts (Köse et al, 2003a(Köse et al, , 2003b, endothelial cells (Shishatskaya and Volova, 2004), and gastrointestinal cells in rats (Freier et al, 2002). Although PHB is inherently biodegradable and biocompatible, the use of PHB is significantly limited in biomedical applications due to several characteristics, such as brittleness, rigidity, and low mechanical properties (Engelberg and Kohn, 1991;Misra et al, 2006).…”

Effect of preparation conditions on morphology, drug content and release profiles of poly(hydroxybutyrate) microparticles containing piroxicam

“…In particular, the polyhydroxyalkanoates (PHAs) have received special attention as promising biomaterials for the preparation of microparticulate drug carriers. [1][2] The first and most reported PHA is poly (3-hydroxybutyrate) [PHB], the degradation of which leads to the formation of 3-hydroxybutiric acid, which is one of the three endogenous ketones produced by a process known as ketogenesis. Also, low molecular weight PHBs, complexed to other macromolecules, are able to pervade aqueous as well as hydrophobic regions of the cells and, thus, they can be found in cytoplasm and intracellular fluids, as well as in membranes and lipoproteins.…”

Forensic Engineering of Advanced Polymeric Materials. Part 1 – Degradation Studies of Polylactide Blends with Atactic Poly[(R,S)-3-hydroxybutyrate] in Paraffin

“…Amorphous forms of the polymer absorb moisture more rapidly than crystalline forms. 28,59 The degradation rate of the PLA-based rigid films depended on the blend miscibility, as described above. The blends with poor miscibility (91PLA/9(R,S)-PHB, 88PLA/12(R,S)-PHB and 85PLA/15(R,S)-PHB rigid films) degraded comparable to the PLA rigid film and faster than the 97PLA/3(R,S)-PHB rigid film, which had good miscibility and a homogeneous surface morphology (see also Fig.…”

“…Atactic PHB degraded faster than the semicrystalline polymer. 28,29 The results show that PHB with a high-molar mass degrades relatively slowly at physiological pH values (7.0-7.4). Random scission of the polymer chains occurs first, followed by mass loss and further decomposition of the samples.…”

“…25,26 A synthetic analogue of aliphatic biopolyester, poly[(R)-3-hydroxybutyrate] -poly[(R,S)-3-hydroxybutyrate] ((R,S)-PHB) -has been recognised to be a suitable modifier for (bio)degradable polymers, such as the brittle polylactide or the highly crystalline polyhydroxyalkanoates (PHA)s of bacterial origin. 27,28 The ring-opening polymerisation of b-butyrolactone with various types of catalysts leads to poly(3-hydroxybutyrate)s of different tacticities, which mimic those polymers found in nature. 29 The hydrolytic degradation products for both (R)-and (S)-3-hydroxybutyrate are nontoxic.…”