In vitro and in vivo evaluation of antidiabetic potential and drug-herb interactions of Euphorbia neriifolia in streptozotocin-induced diabetes in rats and it's in vitro antioxidant studies

Devi, Sushma; Kaur, Navdeep; Kumar, Manish; Kumar, Parveen

doi:10.1016/j.focha.2023.100199

Cited by 7 publications

(4 citation statements)

References 30 publications

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“…The results showed increasing in beta cells numbers and insulin secretion. This result is consistent with the obtained results in current study 46.…”

Section: Discussionsupporting

confidence: 94%

Evaluation of Antioxidants, Antibacterial and Antidiabetic Activities of Aqua-alcoholic Marjoram Extract

Ghudhaib,

Khaleel

2024

Baghdad Sci.J

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Plant extracts possess special significance towards human health and vital activities for a wide range of diseases. The current study aims at verifying the presence of active ingredients in marjoram leaves extract, and determining antioxidants, antibacterial and antifungal activities, LD50 and its antidiabetic activity. The active ingredients were qualitatively analyzed by specific test methods. Antioxidant, LD50, antibacterial and antifungal activities were measured by their standard method. The antidiabetic activity was determined by assessed hyperglycemia, hyperlipidemia and liver functions by spectrophotometric methods. The results confirmed the presence of several active components including flavonoids, alkaloids, terpenoids, tannins, and saponins. Also, the results of antioxidants activity showed that marjoram leaves extract possesses an antioxidant activity similar to ascorbic acid. LD50 results revealed that the allowed dose is 12.5 mg/ml. Biological activity results indicated that marjoram leaves extract possesses antibacterial and antifungal activities against wide range of pathogenic bacteria and fungi, but there is no effect of extract on E. coli and Staphylococcus aureus even at high concentrations. Marjoram extract revealed high antidiabetic activity when treated for two weeks 4mg of the extract daily. Also, lipid profile and liver functions, and testosterone levels were improved after treatment in the diabetic group related to the control group. It can be concluded that marjoram leaves extract has high antioxidant activity with high biological activity and high antidiabetic activity with a significant enhancement of fertility affected in diabetes and a high level of tolerated doses according to the LD50 value.

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“…The results showed increasing in beta cells numbers and insulin secretion. This result is consistent with the obtained results in current study 46.…”

Section: Discussionsupporting

confidence: 94%

Evaluation of Antioxidants, Antibacterial and Antidiabetic Activities of Aqua-alcoholic Marjoram Extract

Ghudhaib,

Khaleel

2024

Baghdad Sci.J

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“…Following anesthesia, the mice were euthanized by cervical decapitation 26 , 27 . Subsequently, the pancreas and liver tissue were isolated, weighed and stored in a deep freezer with a temperature of − 70 °C 28 , 29 .…”

Section: Methodsmentioning

confidence: 99%

Effects of kaempherol-3-rhamnoside on metabolic enzymes and AMPK in the liver tissue of STZ-induced diabetes in mice

Aodah,

Alkholifi,

Alharthy

et al. 2024

Sci Rep

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Diabetes mellitus (DM) is a chronic metabolic disorder characterized by persistent hyperglycemia. It involves disturbances in carbohydrate, fat, and protein metabolism due to defects in insulin secretion, insulin action, or both. Novel therapeutic approaches are continuously being explored to enhance metabolic control and prevent complications associated with the disease. This study investigates the therapeutic potential of kaempherol-3-rhamnoside, a flavonoid, in managing diabetes by modulating the AMP-activated protein kinase (AMPK) pathway and improving metabolic enzyme activities in streptozotocin (STZ) -induced diabetic mice. Diabetic mice were treated with varying doses of kaempherol-3-rhamnoside and/or insulin over a 28-day period. Glycolytic and gluconeogenesis enzyme activities in the liver, fasting blood glucose levels, serum insulin levels, lipid profiles and oxidative stress markers were assessed. Treatment with kaempherol-3-rhamnoside significantly improved glycolytic enzyme activities, reduced fasting blood glucose, and enhanced insulin levels compared to diabetic controls. The compound also normalized lipid profiles and reduced oxidative stress in the liver, suggesting its potential in reversing diabetic dyslipidemia and oxidative damage. Furthermore, kaempherol-3-rhamnoside activated the AMPK pathway, indicating a mechanism through which it could exert its effects. Kaempherol-3-rhamnoside exhibits promising antidiabetic properties, potentially through AMPK pathway activation and metabolic enzyme modulation. These findings support its potential use as an adjunct therapy for diabetes management. Further clinical studies are warranted to validate these results in human subjects.

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“…The mice were then anaesthetised and then euthanised by cervical decapitation. Subsequently, the pancreas and liver tissue were isolated, weighed and stored in a deep freezer with a temperature of -70°C [23][24].…”

Section: Induction Of Diabetesmentioning

confidence: 99%

Effects of kaempherol-3-rhamnoside on metabolic enzymes and AMPK in the Liver Tissue of STZ-Induced Diabetes in Mice

Aodah,

Alkholifi,

Devi

et al. 2024

Preprint

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The current study aimed to investigate the potential antidiabetic properties of kaempherol-3-rhamnoside (Afzelin), both alone and in combination with insulin. To accomplish this, different groups of animals received kaempherol-3-rhamnoside doses and combinations of Humalog insulin and kaempherol-3-rhamnoside for 28 days. The objective was to evaluate the role of kaempherol-3-rhamnoside in glycolytic, gluconeogenic and NADP-linked lipogenic enzymes in liver tissues from STZ-induced diabetic mice while examining pharmacological modulations within the AMPK pathway. These could further regulate metabolic enzymes. The results indicated that in diabetic mice, glycolytic enzyme activities were significantly lower while gluconeogenic ones were higher; however, lipid-based enzyme activity decreased. It was observed that kaempherol-3-rhamnoside had a therapeutic role in the treatment of diabetes by normalising enzyme activities involved in glucose and lipid metabolism. Furthermore, kaempherol-3-rhamnoside treatment activated AMPK activity within liver tissues in diabetic mice by increasing the p-AMPK/AMPK ratio. The inhibited AMPK activity observed in these mice was overcome with this treatment. Additionally, the biochemical analysis indicated that kaempherol-3-rhamnoside has the potential to restore cellular function at the molecular level.

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In vitro and in vivo evaluation of antidiabetic potential and drug-herb interactions of Euphorbia neriifolia in streptozotocin-induced diabetes in rats and it's in vitro antioxidant studies

Cited by 7 publications

References 30 publications

Evaluation of Antioxidants, Antibacterial and Antidiabetic Activities of Aqua-alcoholic Marjoram Extract

Evaluation of Antioxidants, Antibacterial and Antidiabetic Activities of Aqua-alcoholic Marjoram Extract

Effects of kaempherol-3-rhamnoside on metabolic enzymes and AMPK in the liver tissue of STZ-induced diabetes in mice

Effects of kaempherol-3-rhamnoside on metabolic enzymes and AMPK in the Liver Tissue of STZ-Induced Diabetes in Mice

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