In vitro and in vivo technique for assessing vagus nerve regeneration after parietal cell vagotomy in the rat

Joffe, Stephen N.; Crocket, Aysel; Chen, Mei; Brackett, Kim A.

doi:10.1016/0165-1838(83)90130-3

Cited by 8 publications

(5 citation statements)

References 45 publications

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“…Microscopic examinations of the abdominal vagal trunks and their branches below the diaphragm have shown that axons emerging from the proximal stump cross the site of a transection to run both in remnants of the distal stump and intramurally in the wall of the gastrointestinal (GI) tract (see, e.g., Evans and Murray, 1954; Powley et al, 1983; Fox and Powley, 1984; Valentin et al, 1987; Nanobashvili et al, 1994). In addition, from much delayed return of function (e.g., gastric acid secretion: Joffe et al, 1983; Valentin et al, 1987; Nanobashvili et al, 1994; gastric motility: Evans and Murray, 1954; Nanobashvili et al, 1994; insulin secretion: Fox and Powley, 1984; vagally dependent obesity: Fox and Powley, 1984) after experimental transections: It appears that at least some of the plasticity involves reestablishing functional connections.…”

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confidence: 99%

Regenerating vagal afferents reinnervate gastrointestinal tract smooth muscle of the rat

2000

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Peripheral projections of the vagus are known to regenerate after subdiaphragmatic vagotomy, but neither the question of whether the regenerating axons are motor or sensory nor the issue of whether the fibers reinnervate their original targets have been addressed. To determine whether vagal afferents regenerate and whether they differentiate into normal terminal specializations in the reinnervated target organ, male Sprague‐Dawley rats underwent complete subdiaphragmatic vagotomies and were injected 18 weeks later with 3 μl of 4% wheat germ agglutinin‐horseradish peroxidase (WGA‐HRP) in the left nodose ganglion. To provide a comparison group, an unoperated group (controls) was injected with WGA‐HRP in the left nodose ganglion. The esophagus, the entire stomach, the first 8 cm of the duodenum, and the hilus of the liver were prepared as wholemounts and processed with tetramethyl benzidine. Vagal afferents were found to have regenerated and reinnervated the esophagus, stomach, duodenum, and liver. Bundles (two or more axons), individual vagal axons, and terminals in the stomach were counted and mapped with a sampling grid. At 18 weeks postvagotomy, the reinnervated stomach and duodenum contained normal terminals as well as aberrant endings and growth cone profiles. The ingrowing axons reestablished ipsilateral and contralateral projections in the same proportions seen in controls, although the overall density of the different regenerating elements had reached only 7–39% of control values. These findings demonstrate that the gastrointestinal tract and liver can undergo dramatic afferent reinnervation after vagotomy. The presence of differentiated endings at 18 weeks suggests that some afferent function(s) may be restored, and the expression of growth cones suggests that additional regeneration may be ongoing. J. Comp. Neurol. 421:325–346, 2000. © 2000 Wiley‐Liss, Inc.

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confidence: 99%

Regenerating vagal afferents reinnervate gastrointestinal tract smooth muscle of the rat

2000

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“…The cutting of this vascunificant correlation exists between variations in K d and lar branch does not significantly alter gastric acid seserum gastrin concentrations (Fig. 6a, P õ 0.001) and cretion per se [28]. Moreover, the silastic tube placed between B max and serum gastrin levels ( Fig.…”

Section: Correlation Between Serum Gastrin Levels and Gastrin Receptorsmentioning

confidence: 90%

“…PGV was performed under OVA), followed by the Bonferroni test. A time between 8 and 10 ether anesthesia in rats deprived of food for 12 hr following a preweeks had been previously shown to be the period of maximal gastrin viously described procedure [27,28]. Briefly, after medial laparotsecretion in response to PGV in rats.…”

Section: Methodsmentioning

confidence: 99%

“…such as dogs and rabbits, having as it does a well- of the stomach. This distribution facilitates the selective section of the neurovascular bundles innervating this stomach area [27,28,35]. The cutting of this vascunificant correlation exists between variations in K d and lar branch does not significantly alter gastric acid seserum gastrin concentrations (Fig.…”

Section: Correlation Between Serum Gastrin Levels and Gastrin Receptorsmentioning

confidence: 98%

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Time-Dependent Changes of Serum Gastrin and Gastrin Receptors in the Rat Oxyntic Mucosa after Proximal Gastric Vagotomy

Reina¹,

López-Cantarero²,

Gallardo³

et al. 1997

Journal of Surgical Research

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“…Microscopic examinations of the abdominal vagal trunks and their branches below the diaphragm have shown that axons emerging from the proximal stump cross the site of a transection to run both in remnants of the distal stump and intramurally in the wall of the gastrointestinal (GI) tract (see, e.g., Evans and Murray, 1954;Powley et al, 1983;Fox and Powley, 1984;Valentin et al, 1987;Nanobashvili et al, 1994). In addition, from much delayed return of function (e.g., gastric acid secretion: Joffe et al, 1983;Valentin et al, 1987;Nanobashvili et al, 1994;gastric motility: Evans and Murray, 1954;Nanobashvili et al, 1994;insulin secretion: Fox and Powley, 1984; vagally dependent obesity: Fox and Powley, 1984) after experimental transections: It appears that at least some of the plasticity involves reestablishing functional connections.…”

mentioning

confidence: 99%

Regenerating vagal afferents reinnervate gastrointestinal tract smooth muscle of the rat

Phillips¹,

Baronowsky²,

Powley³

2000

J. Comp. Neurol.

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Peripheral projections of the vagus are known to regenerate after subdiaphragmatic vagotomy, but neither the question of whether the regenerating axons are motor or sensory nor the issue of whether the fibers reinnervate their original targets have been addressed. To determine whether vagal afferents regenerate and whether they differentiate into normal terminal specializations in the reinnervated target organ, male Sprague-Dawley rats underwent complete subdiaphragmatic vagotomies and were injected 18 weeks later with 3 microl of 4% wheat germ agglutinin-horseradish peroxidase (WGA-HRP) in the left nodose ganglion. To provide a comparison group, an unoperated group (controls) was injected with WGA-HRP in the left nodose ganglion. The esophagus, the entire stomach, the first 8 cm of the duodenum, and the hilus of the liver were prepared as wholemounts and processed with tetramethyl benzidine. Vagal afferents were found to have regenerated and reinnervated the esophagus, stomach, duodenum, and liver. Bundles (two or more axons), individual vagal axons, and terminals in the stomach were counted and mapped with a sampling grid. At 18 weeks postvagotomy, the reinnervated stomach and duodenum contained normal terminals as well as aberrant endings and growth cone profiles. The ingrowing axons reestablished ipsilateral and contralateral projections in the same proportions seen in controls, although the overall density of the different regenerating elements had reached only 7-39% of control values. These findings demonstrate that the gastrointestinal tract and liver can undergo dramatic afferent reinnervation after vagotomy. The presence of differentiated endings at 18 weeks suggests that some afferent function(s) may be restored, and the expression of growth cones suggests that additional regeneration may be ongoing.

show abstract

In vitro and in vivo technique for assessing vagus nerve regeneration after parietal cell vagotomy in the rat

Cited by 8 publications

References 45 publications

Regenerating vagal afferents reinnervate gastrointestinal tract smooth muscle of the rat

Regenerating vagal afferents reinnervate gastrointestinal tract smooth muscle of the rat

Time-Dependent Changes of Serum Gastrin and Gastrin Receptors in the Rat Oxyntic Mucosa after Proximal Gastric Vagotomy

Regenerating vagal afferents reinnervate gastrointestinal tract smooth muscle of the rat

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