In Vitro and in Vivo Screening of Anti-Inflammatory Activity of Methanolic and Aqueous Extracts of Anogeissus Latifolia Leaves

Abstract

Objective: To evaluate and compare anti-oxidant and anti-inflammatory activity of methanolic and aqueous extract of Anogeissus latifolia leaves Methods: The in vitro antioxidant activity was investigated using nitric oxide radical inhibition activity assay, hydroxyl radical scavenging activity assay, DPPH free radical scavenging assay, and reducing power assay. The in vitro anti-inflammatory activity was investigated using erythrocyte membrane stabilization, inhibition of protein denaturation, and proteinase inhibitory activity the in vivo anti-inflammatory activity was investigated using carrageenan-induced rat paw edema. The biochemical parameters were evaluated in the blood, which included the determination of serum glutamic oxaloacetic transaminase and serum glutamic pyruvic transaminase values and in the liver, which includes the estimation of lipid peroxidation, reduced glutathione, and superoxide dismutase. Results: The methanolic extract caused a significant dose depended on the reduction of inflammation when compared with the aqueous extract of Anogeissus latifolia. The anti-inflammatory activity of all groups was found to be comparable to the standard indomethacin group. The maximum percent inhibition in paw edema was found in methanolic extract of Anogeisuss latifolia at a dose of 500 mg/kg was 53.33%, with significant anti-inflammatory activity p<0.001. Conclusion: The leaf extract of Anogeissus latifolia possesses anti-oxidant and anti-inflammatory activity. The therapeutic effect of Anogeisuss latifolia extracts will encourage its use in the treatment of inflammation.

Bioactivities of Kenaf Biomass Extracts: A Review

“…In their study, aqueous extract and methanolic extract of kenaf leaves at concentrations of 200-400 mg/kg were used to treat carrageenan-induced rat paw oedema. At the highest treatment concentration, kenaf leaf methanolic extract and aqueous leaf methanolic extract showed to inhibit rat paw oedema at 52% and 49%, respectively, which ultimately exemplified the extracts' anti-inflammatory activities [40].…”

“…Thus, the study suggested that kenaf leaf extract may be able to modulate macrophage-mediated responses, which indirectly relates to its immunomodulatory effect. In fact, the inhibition of the inflammatory responses of kenaf leaf extract was proven in vivo by Shaikh, et al [40]. In their study, aqueous extract and methanolic extract of kenaf leaves at concentrations of 200-400 mg/kg were used to treat carrageenan-induced rat paw oedema.…”