In vitro and in vivo study of PCL/COLL wound dressing loaded with insulin-chitosan nanoparticles on cutaneous wound healing in rats model

Ehterami, Arian; Salehi, Majid; Farzamfar, Saeed; Vaez, Ahmad; Samadian, Hadi; Sahrapeyma, Hamed; Mirzaii, Mehdi; Ghorbani, Sadegh; Goodarzi, Arash

doi:10.1016/j.ijbiomac.2018.05.184

Cited by 191 publications

(83 citation statements)

References 31 publications

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“…Ehterami et al (2018) studied the effect of wound dressing loaded with insulin-chitosan nanoparticles on the healing of cutaneous wounds in rats. 31 The results showed that the wounds covered with this dressing reached nearly full closure, compared with those covered with sterile gauze, which exhibited only nearly 45% of wound size reduction. This nanoparticle-loaded wound dressing not only realizes slow release of insulin but also successfully enhances the proliferation of mouse fibroblasts.…”

Section: Animal Studiesmentioning

confidence: 87%

<p>Effects of Topical Insulin on Wound Healing: A Review of Animal and Human Evidences</p>

Wang

2020

DMSO

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Wound healing is a complex biological process that repairs damaged tissues and restores skin integrity. Insulin, a potent factor of wound healing, has been reported for nearly a century to induce rapid recovery of various wounds, as shown by numerous human and animal studies. Although many studies have addressed the healing effect of systemic insulin on burn wound, only few have investigated the efficacy of topical insulin. Thus, this study aimed to review evidence of the effects of topical insulin on wound healing, including on diabetic and non-diabetic wounds. The presented animal and clinical studies support that topical insulin improves wound healing through several mechanisms without causing side effects. Additionally, various wound dressings accelerate the wound healing with controlled and sustained delivery of bioactive insulin. Therefore, topical insulin has been appreciated in field of wound healing, and further studies are needed to improve our understanding of the role of insulin in the healing of various wounds.

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Section: Animal Studiesmentioning

confidence: 87%

<p>Effects of Topical Insulin on Wound Healing: A Review of Animal and Human Evidences</p>

Wang

2020

DMSO

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“…22 In this facet, poly(ε-caprolactone)-chitosan-hydroxyapatite composite membrane scaffolds promoted proliferation and osteogenic differentiation of human dental pulp stem cells. 23 Poly(ε-caprolactone)-chitosan-based composite membranes have been used for wound healing, 24,25 wound dressing, 25 skin repair in burn wounds, 26 cranial tissue regeneration, 27 bladder regeneration, 20 cartilage defect repair, 28 and other applications. Vacanti et al 29 produced poly(ε-caprolactone) and poly(L-lactic)-poly(ε-caprolactone) nanofibers loaded with dexamethasone (5.7 wt.% of dexamethasone relative to dexamethasone and polymer content).…”

Section: Introductionmentioning

confidence: 99%

Chitosan Imparts Better Biological Properties for Poly(ε-caprolactone) Electrospun Membranes than Dexamethasone

Machado

Roberto

Bonafé

et al. 2019

J. Braz. Chem. Soc.

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Poly(ε-caprolactone), an aliphatic polyester with biodegradability and cytocompatibility, has been used to create scaffolds for tissue engineering purposes. However, the hydrophobicity and low water absorptivity of poly(ε-caprolactone) reduce cell anchorage on their membranes. Here, poly(ε-caprolactone)-based scaffolds were prepared by electrospinning of poly(ε-caprolactone)chitosan blend, and poly(ε-caprolactone)-dexamethasone solution. Chitosan and dexamethasone play an essential role to increase the scaffolding performance of poly(ε-caprolactone)-based electrospun membranes. A poly(ε-caprolactone) membrane without chitosan and dexamethasone did not provide satisfactory results to promote cell culture of adipose mesenchymal stem cells (AMSCs). Compared to the poly(ε-caprolactone)-dexamethasone surface, poly(ε-caprolactone)chitosan membrane imparts better cytoskeletal reorganization, and cell spreading, increasing the strength of cell attachment. Also, poly(ε-caprolactone)-chitosan composite provides strong antimicrobial activity against Pseudomonas aeruginosa (ca. 90% inhibition). Therefore, the poly(ε-caprolactone)-chitosan composite is a better alternative to treat skin diseases and promote skin regeneration than conventional approaches based on dexamethasone.

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“…Skin scaffolds are being studied to enhance the control of the wound healing process . In order to fabricate 3D scaffolds that recreate the physiological conditions of the skin, several 3D cell culture systems have been developed employing protein‐based materials, such as collagen, gelatine or silk, among others .…”

Section: Therapeutic Strategies For Skin Regenerationmentioning

confidence: 99%

“…Skin scaffolds are being studied to enhance the control of the wound healing process. [71][72][73] In order to fabricate 3D scaffolds that recreate the physiological conditions of the skin, several 3D cell culture systems have been developed employing proteinbased materials, such as collagen, gelatine or silk, among others. 74,75 These materials have proven to be advantageous since they are made of biomolecules similarly found in natural tissues and can be degraded and cleared by the host's native physiological processes.…”

Section: D Scaffoldsmentioning

confidence: 99%

Therapeutic strategies for skin regeneration based on biomedical substitutes

Chocarro‐Wrona

López‐Ruiz

Perán

et al. 2019

Acad Dermatol Venereol

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Regenerative medicine and tissue engineering (TE) have experienced significant advances in the development of in vitro engineered skin substitutes, either for replacement of lost tissue in skin injuries or for the generation of in vitro human skin models to research. However, currently available skin substitutes present different limitations such as expensive costs, abnormal skin microstructure and engraftment failure. Given these limitations, new technologies, based on advanced therapies and regenerative medicine, have been applied to develop skin substitutes with several pharmaceutical applications that include injectable cell suspensions, cell‐spray devices, sheets or 3Dscaffolds for skin tissue regeneration and others. Clinical practice for skin injuries has evolved to incorporate these innovative applications to facilitate wound healing, improve the barrier function of the skin, prevent infections, manage pain and even to ameliorate long‐term aesthetic results. In this article, we review current commercially available skin substitutes for clinical use, as well as the latest advances in biomedical and pharmaceutical applications used to design advanced therapies and medical products for wound healing and skin regeneration. We highlight the current progress in clinical trials for wound healing as well as the new technologies that are being developed and hold the potential to generate skin substitutes such as 3D bioprinting‐based strategies.

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In vitro and in vivo study of PCL/COLL wound dressing loaded with insulin-chitosan nanoparticles on cutaneous wound healing in rats model

Cited by 191 publications

References 31 publications

<p>Effects of Topical Insulin on Wound Healing: A Review of Animal and Human Evidences</p>

<p>Effects of Topical Insulin on Wound Healing: A Review of Animal and Human Evidences</p>

Chitosan Imparts Better Biological Properties for Poly(ε-caprolactone) Electrospun Membranes than Dexamethasone

Therapeutic strategies for skin regeneration based on biomedical substitutes

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