Chitosan Imparts Better Biological Properties for Poly(ε-caprolactone) Electrospun Membranes than Dexamethasone

Abstract

Poly(ε-caprolactone), an aliphatic polyester with biodegradability and cytocompatibility, has been used to create scaffolds for tissue engineering purposes. However, the hydrophobicity and low water absorptivity of poly(ε-caprolactone) reduce cell anchorage on their membranes. Here, poly(ε-caprolactone)-based scaffolds were prepared by electrospinning of poly(ε-caprolactone)chitosan blend, and poly(ε-caprolactone)-dexamethasone solution. Chitosan and dexamethasone play an essential role to increase the scaffolding performance of poly(ε-caprolactone)-based electrospun membranes. A poly(ε-caprolactone) membrane without chitosan and dexamethasone did not provide satisfactory results to promote cell culture of adipose mesenchymal stem cells (AMSCs). Compared to the poly(ε-caprolactone)-dexamethasone surface, poly(ε-caprolactone)chitosan membrane imparts better cytoskeletal reorganization, and cell spreading, increasing the strength of cell attachment. Also, poly(ε-caprolactone)-chitosan composite provides strong antimicrobial activity against Pseudomonas aeruginosa (ca. 90% inhibition). Therefore, the poly(ε-caprolactone)-chitosan composite is a better alternative to treat skin diseases and promote skin regeneration than conventional approaches based on dexamethasone.

Development of a Bioinspired, Self‐Adhering, and Drug‐Eluting Laryngotracheal Patch

“…To fabricate dexamethasone‐loaded polycaprolactone (PCL) electrospun fibers, PCL pellets (M W : 80,000) were dissolved in chloroform and ethanol (15:85 v/v) and dexamethasone (1:10 Dexamethasone:PCL w/w) was added 32 . Final solutions were electrospun at a traveling distance 30 cm, flow rate 1.4 ml/hr, and applied voltage of 25 KV.…”

Polysaccharide-Based Materials Created by Physical Processes: From Preparation to Biomedical Applications

“…This section reviews polysaccharide-based assemblies applied as scaffolds, wound dressings, surface coatings, and DDSs for GF delivery [ 54 , 205 , 206 ]. Cytocompatible scaffolds can be formulated into 3D porous structures with controlled biodegradation rates in biological environments.…”

“…Polysaccharide-based materials often have weak mechanical properties, and chitosan-based assemblies need to be washed to remove residual H 3 O + contents [ 138 , 145 ]. Overall, the mechanical properties of the physical assemblies are improved by associating them with gelatin [ 54 , 195 ], γ-polyglutamic acid [ 225 ], polyethylene oxide [ 195 ], poly(ε-caprolactone) [ 206 , 223 , 224 ], poly(ethylene glycol) [ 229 ], poly(vinyl alcohol) [ 227 , 228 ], polyethyleneimine [ 226 ], and Manuka honey [ 205 ]. For example, Manuka honey (2.0 w / v ) significantly increases the elastic modulus of gellan gum scaffolds stabilized with Ca(II) ions to 116 kPa.…”

“…Reprinted with permission from References [ 138 , 145 ]; published by Elsevier, 2018 and 2020, respectively. Reprinted with permission from Reference [ 206 ]; published by SciELO, 2019.…”

Kinetically guided high‐yield and rapid production of ε‐caprolactone in a microreactor system

“…Up to now, more than 15 countries and regions have issued policies to “ban plastic” or “limit plastic.” As a result, significant efforts have been made to develop environmentally friendly plastics that can degrade in the environment without leaving harmful products 2 . Among these degradable polymers, polycaprolactone (PCL) has attracted great attention due to its excellent biodegradability and biocompatibility and has been widely used as surgical sutures, stents, and drug carriers 3–11 . However, full commercial development of the PCL has been discouraged by the absence of an efficient and safe method to produce its monomer, ε‐caprolactone (ε‐CL) 12 .…”