In Vitro and in Silico Assessment of the Potential of Niaouli Essential Oil as a Quorum Sensing Inhibitor of Biofilm Formation and its Effects on Fibroblast Cell Viability

Abstract

Essential oils (EO), as new bioactive compounds, have been used for pharmaceutical applications. In this study, EO of Niaouli was found to have a high content in 1,8-cineole (58.53%). Furthermore, pinene, α-terpineol, nerolidol and ledene were found to be its components with an abundance of above 2%. Niaouli EO also had effects as inhibitor of Pseudomonas aeruginosa PAO1 biofilm formation (p<0.05). In the molecular docking study, this effect was explored. The natural ligand OdDHL, the bound ligand TP-1 (Triphenyl-1), the major component 1,8-cineole and the other components with significant abundance were docked against the binding region of the LasR protein. The docking study exhibited that 1,8-cineole together with the other components investigated could inhibit LasR competitively. Its effect on cell viability was also HIGHLIGHTS• Essential oil (EO) of Niaouli is an inhibitor on biofilm formation of P. aeruginosa.• The main components of Niaouli EO act as possible inhibitors of LasR.• The inhibition effect of these molecules on LasR is verified by molecular docking.• Niaouli EO has effects on cell viability of fibroblast cells at high concentrations.• Niaouli is a valuable essential oil for pharmaceutical applications.Onem, E; et al.

PVA/Inulin-Based Sustainable Films Reinforced with Pickering Emulsion of Niaouli Essential Oil for Potential Wound Healing Applications

“…Furthermore [ 64 ], Donoyama and Ichiman (2006) reported that niaouli oil has antibacterial effects on Staphylococcus aureus . Niaouli essential oil was effective against pathogenic microorganisms resistant to antibiotics on the skin surface, and the effectiveness of P. aeruginosa on biofilm formation was determined by [ 65 , 66 ]. In another study, Ozdemir et al (2018) found that niaouli had strong antibacterial resistance of 13.0 ± 1.00 mm, 20.0 ± 1.00 mm, 21.0 ± 1.00 mm, 15.0 ± 1.00 mm, 17.0 ± 1.00 mm, 15.0 ± 1.00 mm, 35.0 ± 0.57 mm, and 13.0 ± 1.00 mm against Pseudomonas aeruginosa , Staphylococcus aureus , Escherichia coli , Enterococcus faecalis , Bacillus subtilis , Salmonella typhimurium , Staphylococcus epidermidis , and Enterococcus hirae , respectively [ 67 ].…”

Anti-quorum sensing and cytotoxic activity of elemi essential oil

“…Numerous studies have been conducted on the antimicrobial effects of essential oil components. In addition to the antibacterial activity of the essential oil, anti-quorum sensing activity has also been reported in many studies (Onem et al, 2021;Sobrinho et al, 2020). In recent years, anti-quorum sensing activity is an approach that is thought to be effective in combating infectious diseases due to antibiotic resistance (Algburi et al, 2017;Alva et al, 2019;Millezi et al, 2016;Roy et al, 2018).…”

The Molecular Architecture of Pseudomonas aeruginosa Quorum-Sensing Inhibitors

“…1,8-Cineole is the main component of niaouli essential oil, and it shows an inhibitory effect on biofilm formation in P. aeruginosa . A molecular docking study confirmed that it interfered the binding with LasR and inhibited las [ 28 ]. Some catabolites were reported to inhibit quorum sensing by inhibiting las .…”