In vitro and in silico inhibition properties of fucoidan against α-amylase and α-D-glucosidase with relevance to type 2 diabetes mellitus

Selvaraj, Senthil; Chandrasekaran, Raghu; Arjun, H. A.; Anantharaman, P.

doi:10.1016/j.carbpol.2019.01.039

Cited by 93 publications

(10 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Various in vitro studies showed that low-molecular-weight bioactive polysaccharides obtained from the fruits of blackcurrant (Ribes nigrum L.), an alkaline soluble polysaccharide from Coreopsis tinctoria ; fucoidan polysaccharide from Turbinaria conoides ; polysaccharide fraction from Diaphragma juglandis fructus exhibited higher antioxidant, α-amylase, and α-glucosidase inhibitory activities that showed higher bioactive, with hypoglycemic, potential [134,135,136,137]. Furthermore, these polysaccharides significantly prevented the synthesis of NO, TNF-α, and IL-6 in LPS (Lipopolysaccharide)-stimulated BV2 (raf/myc-immortalised murine neonatal microglial cell line) microglial cells [134].…”

Section: Mechanism Of Dietary Polysaccharides On Anti-diabetic Actmentioning

confidence: 99%

Anti-Diabetic Effects and Mechanisms of Dietary Polysaccharides

Ganesan

2019

Molecules

106

View full text Add to dashboard Cite

Diabetes mellitus is a multifactorial, heterogeneous metabolic disorder, causing various health complications and economic issues, which apparently impacts the human’s life. Currently, commercial diabetic drugs are clinically managed for diabetic treatment that has definite side effects. Dietary polysaccharides mainly derive from natural sources, including medicinal plants, grains, fruits, vegetables, edible mushroom, and medicinal foods, and possess anti-diabetic potential. Hence, this review summarizes the effects of dietary polysaccharides on diabetes and underlying molecular mechanisms related to inflammatory factors, oxidative stress, and diabetes in various animal models. The analysis of literature and appropriate data on anti-diabetic polysaccharide from electronic databases was conducted. In vivo and in vitro trials have revealed that treatment of these polysaccharides has hypoglycemic, hypolipidemic, antioxidant, and anti-inflammatory effects, which enhance pancreatic β-cell mass and alleviates β-cell dysfunction. It enhances insulin signaling pathways through insulin receptors and activates the PI3K/Akt pathway, and eventually modulates ERK/JNK/MAPK pathway. In conclusion, dietary polysaccharides can effectively ameliorate hyperglycemia, hyperlipidemia, low-grade inflammation, and oxidative stress in type 2 diabetes mellitus (T2DM), and, thus, consumption of polysaccharides can be a valuable choice for diabetic control.

show abstract

Section: Mechanism Of Dietary Polysaccharides On Anti-diabetic Actmentioning

confidence: 99%

Anti-Diabetic Effects and Mechanisms of Dietary Polysaccharides

Ganesan

2019

Molecules

106

View full text Add to dashboard Cite

show abstract

“…Alginate, a polysaccharide distributed in the cell wall of brown algae, has beneficial effects on glucose metabolism [9]. Fucoidan, a sulfated polysaccharide also found in various species of brown algae, lowers alpha-glucosidase activity in vitro [10], blood glucose in db/db mice [11], and glycated hemoglobin and glucagon-like peptide-1 in type 2 diabetes patients [12]. The phlorotannin, dieckol, isolated from brown seaweed, was found to inhibit alpha-glucosidase activity in vitro [13], and to protect against the glucotoxicity-induced oxidative stress associated with diabetes [14,15], delay T2D development in a db/db mouse model [16], and reduce postprandial hyperglycemia in prediabetic subjects [17].…”

Section: Introductionmentioning

confidence: 99%

Nordic Seaweed and Diabetes Prevention: Exploratory Studies in KK-Ay Mice

et al. 2019

View full text Add to dashboard Cite

Background: The global epidemic of type 2 diabetes (T2D) is a challenging health problem. Lifestyle changes, including nutrition therapy, areimportant for the prevention and management of T2D. Seaweeds contain several bioactive substances with potential health properties and may be a low-cost alternative functional food in the prevention of T2D. Objective: The aim of this study was to explore the preventive effects of dried Nordic seaweed species on diabetes in an animal model of T2D. Method: Fiftymale KK-Ay mice were randomly assigned to one of four diets: control diet (chow) or diets supplemented with Alaria esculenta (AE), Saccharina latissima (SL), or Palmaria palmata (PP). The effect of the interventions on the progression of T2D was monitored over 10 weeks and evaluated by circulating glucose, glycated hemoglobin (HbA1c), insulin, glucagon, and lipid levels. Results: The SL group had significantly lower bodyweight, lower HbA1c and insulin levels, as well as higher high density lipoprotein (HDL) cholesterol levels after the 10-week intervention than the control group. At the end of the study, the control group had significantly higher HbA1c (p < 0.001) than all of the seaweed groups. Conclusion: All seaweed groups improved HbA1C compared to control and Saccharinalatissima seaweed had concomitantly beneficial effects on glycemic control and lipid levels in KK-Ay diabetic mice.

show abstract

“…Additionally, a number of hydrophobic and noncovalent interactions such as π -cation (with residue Lys191) and π - π stacking (with residue Ala218), together with van der Waals forces (with residues Pro211 and Trp226), are responsible for the affinity and subsequent stability of trilobine in the active pocket of glucosidase. Such bonds and interactions have been reported to stabilize amylase- and glucosidase-ligands relationships in previous studies [ 32 , 33 ]. The observed favourable interaction could account for the lower binding energies (-10.0 and -10.0 kcal/mol) for amylase and glucosidase, respectively.…”

Section: Discussionmentioning

confidence: 75%

Chemical Group Profiling, In Vitro and In Silico Evaluation of Aristolochia ringens on α-Amylase and α-Glucosidase Activity

Ahmad

Ajani

Sabiu

2021

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

Diabetes mellitus (DM) has become a global scourge, and there is a continuous search for novel compounds as viable alternatives to synthetic drugs which are often accompanied by severe adverse effects. Aristolochia ringens is among the scientifically implicated botanicals effective in the management of several degenerative diseases including DM. The current study evaluated the inhibitory mechanism(s) of root extract of A. ringens on α-amylase and α-glucosidase in vitro and in silico, while its constituents were characterized using liquid chromatography-mass spectrometric technique. The extract had concentration-dependent inhibitory effect on the study enzymes, and the inhibition compared well with that of standard drug (acarbose) with respective IC50 values of 0.67 mg/mL (α-amylase) and 0.57 mg/mL (α-glucosidase) compared with that of the extract (0.63 and 0.54 mg/mL). The extract competitively and uncompetitively inhibited α-amylase and α-glucosidase, respectively. Of the identified compounds, dianoside G (−12.4, −12.5 kcal/mol) and trilobine (−10.0, −10.0 kcal/mol) had significant interactions with α-amylase and α-glucosidase, respectively, while magnoflorine and asiatic acid also interacted keenly with both enzymes, with quercetin 3-O-glucuronide and strictosidine showing better affinity towards α-glucosidase. These observations are suggestive of involvement of these compounds as probable ligands contributing to antidiabetic potential of the extract. While studies are underway to demystify the yet to be identified compounds in the extract, the data presented have lent scientific credence to the acclaimed in vivo antidiabetic potential of the extract and suggested it as a viable source of oral hypoglycaemic agent.

show abstract

In vitro and in silico inhibition properties of fucoidan against α-amylase and α-D-glucosidase with relevance to type 2 diabetes mellitus

Cited by 93 publications

References 31 publications

Anti-Diabetic Effects and Mechanisms of Dietary Polysaccharides

Anti-Diabetic Effects and Mechanisms of Dietary Polysaccharides

Nordic Seaweed and Diabetes Prevention: Exploratory Studies in KK-Ay Mice

Chemical Group Profiling, In Vitro and In Silico Evaluation of Aristolochia ringens on α-Amylase and α-Glucosidase Activity

Contact Info

Product

Resources

About