In vitro and in silico antioxidant and toxicological activities of Achyrocline satureioides

Salgueiro, Andréia Caroline Fernandes; Folmer, Vanderlei; Rosa, Hemerson Silva da; Costa, Márcio Tavares; Boligon, Aline Augusti; Paula, Fávero Reisdorfer; Roos, Daniel Henrique; Puntel, Gustavo Orione

doi:10.1016/j.jep.2016.08.048

Cited by 42 publications

(27 citation statements)

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“…The presence of active flavonoids in both extracts explains the biological activities found in this study, which was based on in vitro and in silico predictions with natural products [ 32 ]. Antioxidants could have a pathogen neutralizing action, directly by scavenging ROS or indirectly by activating pathways that promote ROS degradation [ 27 ].…”

Section: Resultsmentioning

confidence: 98%

Flavonoid Composition and Biological Activities of Ethanol Extracts of Caryocar coriaceum Wittm., a Native Plant from Caatinga Biome

Alves

Morais

Tomiotto-Pellissier

et al. 2017

Evidence-Based Complementary and Alternative Medicine

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Caryocar coriaceum fruits, found in Brazilian Cerrado and Caatinga, are commonly used as food and in folk medicine, as anti-inflammatory, bactericide, fungicide, leishmanicide, and nematicide. Due to the biological potential of this plant, this study focuses on the evaluation of antifungal and antileishmanial activities, including anticholinesterase and antioxidant tests, correlating with total phenols and flavonoids content. Peel extracts contain higher yield of phenols and flavonoids as analyzed by spectrophotometric methods. HPLC analysis of flavonoids revealed that isoquercitrin is the main flavonoid in both parts of the fruit, and peel extract showed the best antioxidant activity. In the inhibition of the acetylcholinesterase assay, both extracts demonstrate action comparable to physostigmine. The antimicrobial activity of extracts was evaluated against strains of Malassezia sp. and Microsporum canis, using the broth microdilution technique, in which the extracts showed similar MIC and MFC. The extracts present antileishmanial activity and low toxicity on murine macrophages and erythrocytes. Therefore, these results suggest a potential for the application of C. coriaceum fruit's ethanol extracts in the treatment against dermatophyte fungi and leishmaniasis, probably due to the presence of active flavonoids. Further in vivo studies are recommended aiming at the development of possible new pharmaceutical compounds.

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Section: Resultsmentioning

confidence: 98%

Flavonoid Composition and Biological Activities of Ethanol Extracts of Caryocar coriaceum Wittm., a Native Plant from Caatinga Biome

Alves

Morais

Tomiotto-Pellissier

et al. 2017

Evidence-Based Complementary and Alternative Medicine

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“…observed the presence of glycosylated kaempferol. However, recently Salgueiro et al . reported its presence as kaempferol‐aglycone.…”

Section: Resultsmentioning

confidence: 99%

Semi‐preparative isolation and purification of phenolic compounds from Achyrocline satureioides (Lam) D.C. by high‐performance counter‐current chromatography

Bianchi

Kaiser

Pittol

et al. 2018

Phytochemical Analysis

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Introduction Phenolic compounds present in Achyrocline satureioides are known to have therapeutic benefits like antioxidant, anti‐inflammatory, and antitumour properties. The main polyphenols present in the plant are quercetin (QCT), luteolin (LUT), 3‐O‐methylquercetin (3OMQ), and achyrobichalcone (ACB). However, the effective isolation and purification of these compounds from A. satureioides inflorescences are not an easy task. Objective To develop an efficient high‐performance counter‐current chromatography (HPCCC) method for quick separation and purification of naturally occurring phenolic compounds from the extract of A. satureioides. Methodology A two‐step HPCCC semi‐preparative isolation method was developed using a solvent system composed of n‐hexane/ethyl acetate/methanol/water (0.8:1.0:0.8:1.0) and dichloromethane/methanol/water (3.5:3.5:2.5). Results The HPCCC method was used to obtain two fractions. The first fraction (F1) contained high levels of ACB, among other constituents, while the second fraction (F2) contained mostly QCT, LUT, and 3OMQ. Besides the high ACB content, F1 contained three other flavonoid‐aglycones (kaempferol, 97.3%; isokaempferide, 92.4%; and 3,3′‐di‐O‐methylquercetin, 95.2%) identified by an ultra‐performance liquid chromatography system coupled to a quadrupole time‐of‐flight with high‐definition mass spectrometry (UPLC‐QTOF/HDMS) and nuclear magnetic resonance (NMR) analysis. Purity levels of ACB, 3OMQ, QCT, and LUT were 98.0, 97.0, 97.5, and 90.2%, respectively. Conclusion This is the first time that high purity ACB and six other flavonoids were obtained from A. satureioides inflorescences by HPCCC. These excellent results reveal the potential and versatility of HPCCC as a technique to produce different types of products from this plant species on a semi‐preparative scale: enriched fractions, new metabolites, or high purity compounds.

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“…For toxic risk prediction, four online computer programs: ACD/Labs (Toronto, ON, Canada), admetSAR server, pkCSM platform, and the PreADMET web-based application () were employed. The toxic risks assessed were interpreted and expressed in a flexible manner: (+) low potential, (++) medium risk, (+++) high risk and non-detected risk (N) [58]. For biological activities spectrum prediction, PASS (available in ) was used.…”

Section: Methodsmentioning

confidence: 99%

Lannea coromandelica (Houtt.) Merr. Induces Heme Oxygenase 1 (HO-1) Expression and Reduces Oxidative Stress via the p38/c-Jun N-Terminal Kinase–Nuclear Factor Erythroid 2-Related Factor 2 (p38/JNK–NRF2)-Mediated Antioxidant Pathway

Alam

Kwon

Lee

et al. 2017

IJMS

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The leaves of Lannea coromandelica (Houtt.) Merr. are used in the Garo, Pahan, and Teli tribal communities of Bangladesh as a traditional medicinal plant to treat hepatitis, diabetes, ulcers, heart disease, and dysentery. However, there have been limited phytochemical and biological studies on the bark of L. coromandelica. This study aimed to investigate the antioxidant activities of L. coromandelica bark extract (LCBE) and the underlying mechanism using RAW 264.7 cells. The LCBE was analysed by high-pressure liquid chromatography (HPLC) to detect its key polyphenolic compounds. Various in vitro antioxidant assays were performed using RAW 264.7 cells to assess the antioxidant effects of the LCBE and to understand the underlying molecular mechanism. HPLC revealed the presence of gallic acid, (−)-epigallocatechin-3-gallate, catechin, chlorogenic acid, and caffeic acid in the LCBE. The extract showed a very potent capacity to scavenge numerous free radicals through hydrogen atom transfer and/or electron donation and also quenched cellular reactive oxygen species (ROS) generation without showing any toxicity. The LCBE was found to combat the oxidative stress by enhancing the expression, at both transcriptional and translational levels, of primary antioxidant enzymes as well as phase II detoxifying enzymes, especially heme oxygenase 1, through the upregulation of the nuclear factor erythroid 2-related factor 2 (NRF2)-mediated pathway in RAW 264.7 cells via the phosphorylation of p38 kinase and c-Jun N-terminal kinase (JNK). The LCBE exhibited strong antioxidant activities and mitigated the cellular ROS production. These results provide scientific evidence of its potential as an ideal applicant for a cost-effective, readily available, and natural phytochemical, as well as a strategy for preventing diseases associated with oxidative stress and attenuating disease progress.

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In vitro and in silico antioxidant and toxicological activities of Achyrocline satureioides

Abstract: Our results indicate that A. satureioides infusion possesses low toxicological potential and an effective antioxidant activity. These findings confirm the traditional use of this plant in the folk medicine.

Cited by 42 publications

References 42 publications

Flavonoid Composition and Biological Activities of Ethanol Extracts of Caryocar coriaceum Wittm., a Native Plant from Caatinga Biome

Flavonoid Composition and Biological Activities of Ethanol Extracts of Caryocar coriaceum Wittm., a Native Plant from Caatinga Biome

Semi‐preparative isolation and purification of phenolic compounds from Achyrocline satureioides (Lam) D.C. by high‐performance counter‐current chromatography

Lannea coromandelica (Houtt.) Merr. Induces Heme Oxygenase 1 (HO-1) Expression and Reduces Oxidative Stress via the p38/c-Jun N-Terminal Kinase–Nuclear Factor Erythroid 2-Related Factor 2 (p38/JNK–NRF2)-Mediated Antioxidant Pathway

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