In vitro amoebicidal activity of poly(maleic anhydride-co-vinyl acetate) copolymer on Acanthamoeba spp. trophozoites and cysts

Karakuş, Gülderen; Malatyalı, Erdoğan; Zengin, Hacı Bayram; Değerlı, Serpil

doi:10.12808/bcs.v2i1.16

Cited by 4 publications

(1 citation statement)

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“…Detail information of all of these synthesis process of MAVA could be followed in our previous study 12 . Briefly, drug carrier MAVA copolymer also known as poly(Maleic anhydride-co-Vinyl acetate), was synthesized by free-radical polymerization of maleic anhydride (MA) and vinyl acetate (VA), at a 1:1 molar ratio.…”

Section: Preparation Of Mava Copolymermentioning

confidence: 99%

Cytotoxic and apoptotic effects of Poly(maleic anhydride-co-vinyl acetate) drug carrier copolymer on MCF-7 and MDA-MB-231 breast cancer cells

İnan¹,

Bölükbaşı

Karakuş

2019

Cumhuriyet Medical Journal

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In recent years, copolymers are frequently used in many areas. The biocompatibility of any copolymer should be examined for practical application. One of these copolymers is Poly [(maleic anhydride)-co-(vinyl acetate)] (MAVA), and the usage area of MAVA is quite limited. In this study, the cytotoxic effect of MAVA on MDA-MB-231 and MCF-7 human breast cancer cells was determined by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), and apoptotic cells are marked with DAPI staining. For this purpose, MDA-MB-231 and MCF-7 human breast cancer cells were incubated with different concentrations of MAVA

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Section: Preparation Of Mava Copolymermentioning

confidence: 99%

Cytotoxic and apoptotic effects of Poly(maleic anhydride-co-vinyl acetate) drug carrier copolymer on MCF-7 and MDA-MB-231 breast cancer cells

İnan¹,

Bölükbaşı

Karakuş

2019

Cumhuriyet Medical Journal

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Amebicidal effects of fenugreek (Trigonella foenum‐graecum) against Acanthamoeba cysts

Kaya

Baldemir

Karaman

et al. 2019

Food Science & Nutrition

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Trigonella foenum‐graecum L. (TF) is known to the public as a chest emollient, mucous expectorant, laxative and is used to prevent maturation of boils and diabetes since ancient times. In this study, we aimed to determine the amebicidal effects against Acanthamoeba cysts. Plant extracts were prepared at concentrations of 1, 2, 4, 8, 16, and 32 mg/ml and were placed in a hemocytometer with cell counts 22 × 106 cell/ml. The fatty acid profiles of TF seeds were determined. Standard Acanthamoeba cysts were added and incubated at 25°C. The viability of the parasite was checked and recorded at hours 3, 24, 48, 72, 96, and 102. The values of lethal concentration doses (LD50 and LD90) were calculated using probit analysis. This study revealed that T. foenum‐graecum prevented proliferation of the parasite at certain times. However, further for in vivo and controlled experimental studies are needed in order to find out how to use this plant as medication.

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Challenges for Therapeutic Application of Pseudomonas Exotoxin-Based Immunotoxins

Benhar

2015

Resistance to Targeted Anti-Cancer Therapeutics

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Immunotoxins are therapeutic molecules that belong to a class of biopharmaceuticals called "Armed antibodies". Immunotoxins are based on very potent toxins of bacterial or plant origin that lack target-cell specificity. To make them target-cell-specific, the non-specific cell binding domains of the original toxins are replaced with a target-cell-specific binding protein, in most cases a monoclonal antibody or a recombinant antibody fragment. The most clinically-advanced immunotoxins are currently being evaluated in phase II and III clinical studies. Like other targeted and non-targeted therapeutics, immunotoxins too suffer from several limitations that may hinder their therapeutic efficacy. Such limitations include, but are not limited to immunogenicity, modification of the extracellular target to which the targeting antibody binds, modification of the intracellular target upon which the toxin acts to cause cell growth inhibition, and insufficient potency as single agents and off-target toxicity, where non-target cells and organs are affected by the immunotoxin, severely impairing its therapeutic index. This chapter is devoted to a group of immunotoxins in which the toxic moiety is derived from exotoxin A (PE) of the bacterium Pseudomonas aeruginosa. The limitations to the efficacy of PE-based immunotoxins, as well as potential solutions for overcoming such limitations, will be presented. Chapter 2 of this book: "Resistance of tumor cells against antibodytargeted protein toxins" by Ulrich Brinkmann et al. is focused on factors that influence the sensitivity or potential resistances of cancer cells towards recombinant immunotoxins which carry truncated and/or mutated derivatives of Pseudomonas exotoxin as cytotoxic payloads.

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In vitro amoebicidal activity of poly(maleic anhydride-co-vinyl acetate) copolymer on Acanthamoeba spp. trophozoites and cysts

Abstract: This is an open-access article distributed under the terms of the Creative Common Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.

Cited by 4 publications

References 34 publications

Cytotoxic and apoptotic effects of Poly(maleic anhydride-co-vinyl acetate) drug carrier copolymer on MCF-7 and MDA-MB-231 breast cancer cells

Cytotoxic and apoptotic effects of Poly(maleic anhydride-co-vinyl acetate) drug carrier copolymer on MCF-7 and MDA-MB-231 breast cancer cells

Amebicidal effects of fenugreek (Trigonella foenum‐graecum) against Acanthamoeba cysts

Challenges for Therapeutic Application of Pseudomonas Exotoxin-Based Immunotoxins

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